Abstract: The present invention provides biological compositions and methods for making the same.

Abstract: A centrifugal method, and corresponding system, for processing blood to collect platelet rich plasma. A separation chamber is filled with blood from a fill syringe by rotating the separation chamber at a fill rotation rate and pumping the blood from the fill syringe. A soft spin is used to initially separate red blood cells from platelets by spinning the separation chamber at a soft spin rate. A percentage of the blood is drawn from the separation chamber back into the fill syringe to remove separated red blood cells. A second portion of the separated blood is drawn from the separation chamber until a red blood cell/platelet interface is detected. A hard spin is performed by spinning the separation chamber at a higher rate and connecting tubing is cleared of red blood cells by drawing a predetermined clearing volume. The platelet rich plasma is then collected in the collection syringe.

Abstract: A centrifugal method, and corresponding system, for processing blood to collect platelet rich plasma. A separation chamber is filled with blood from a fill syringe by rotating the separation chamber at a fill rotation rate and pumping the blood from the fill syringe. A soft spin is used to initially separate red blood cells from platelets by spinning the separation chamber at a soft spin rate. A percentage of the blood is drawn from the separation chamber back into the fill syringe to remove separated red blood cells. A second portion of the separated blood is drawn from the separation chamber until a red blood cell/platelet interface is detected. A hard spin is performed by spinning the separation chamber at a higher rate and connecting tubing is cleared of red blood cells by drawing a predetermined clearing volume. The platelet rich plasma is then collected in the collection syringe.

Abstract: A centrifugal method, and corresponding system, for processing blood to collect platelet rich plasma. A separation chamber is filled with blood from a fill syringe by rotating the separation chamber at a fill rotation rate and pumping the blood from the fill syringe. A soft spin is used to initially separate red blood cells from platelets by spinning the separation chamber at a soft spin rate. A percentage of the blood is drawn from the separation chamber back into the fill syringe to remove separated red blood cells. A second portion of the separated blood is drawn from the separation chamber until a red blood cell/platelet interface is detected. A hard spin is performed by spinning the separation chamber at a higher rate and connecting tubing is cleared of red blood cells by drawing a predetermined clearing volume. The platelet rich plasma is then collected in the collection syringe.

Abstract: A centrifugal method, and corresponding system, for processing blood to collect platelet rich plasma. A separation chamber is filled with blood from a fill syringe by rotating the separation chamber at a fill rotation rate and pumping the blood from the fill syringe. A soft spin is used to initially separate red blood cells from platelets by spinning the separation chamber at a soft spin rate. A percentage of the blood is drawn from the separation chamber back into the fill syringe to remove separated red blood cells. A second portion of the separated blood is drawn from the separation chamber until a red blood cell/platelet interface is detected. A hard spin is performed by spinning the separation chamber at a higher rate and connecting tubing is cleared of red blood cells by drawing a predetermined clearing volume. The platelet rich plasma is then collected in the collection syringe.

Abstract: A centrifugal method, and corresponding system, for processing blood to collect platelet rich plasma. A separation chamber is filled with blood from a fill syringe by rotating the separation chamber at a fill rotation rate and pumping the blood from the fill syringe. A soft spin is used to initially separate red blood cells from platelets by spinning the separation chamber at a soft spin rate. A percentage of the blood is drawn from the separation chamber back into the fill syringe to remove separated red blood cells. A second portion of the separated blood is drawn from the separation chamber until a red blood cell/platelet interface is detected. A hard spin is performed by spinning the separation chamber at a higher rate and connecting tubing is cleared of red blood cells by drawing a predetermined clearing volume. The platelet rich plasma is then collected in the collection syringe.

Abstract: The present invention provides a tubing set in cassette form and an irrigation system employing the cassette and tubing set for use in endoscopic procedures. The tubing set includes a generally arcuate cassette which is inserted into the pump housing to effect a change in tubing sets between procedures. A valve assembly and flattened tubing segment are preferably used to provide pressure control/relief in the tubing set/irrigation system. Also provided in combination with the preferred cassette is a fitting for coupling a pressure monitoring line with pressure monitoring equipment in the irrigation system.

Abstract: A cardioplegia administration system includes a tubing set, a positive displacement pump and a mixing system. The tubing set has a cardioplegia supply tube; a blood supply tube; and a cardioplegia administration tube connected to the cardioplegia and blood supply tubes. The positive displacement pump engages the cardioplegia administration tube to pump fluid therethrough. The mixing system includes pinch valves for alternately-continually pinching the cardioplegia and blood supply tubes to close and open the cardioplegia and blood supply tubes such that only one of the cardioplegia and blood supply tubes is open at a time, and a controller that controls the intervals during which the pinch valves are open to control the ratio of the cardioplegia medication and blood or blood substitute administered through the cardioplegia administration tube.

Abstract: A cardioplegia administration system includes a tubing set, a positive displacement pump and a mixing system. The tubing set has a cardioplegia supply tube; a blood supply tube; and a cardioplegia administration tube connected to the cardioplegia and blood supply tubes. The positive displacement pump engages the cardioplegia administration tube to pump fluid therethrough. The mixing system includes pinch valves for alternately-continually pinching the cardioplegia and blood supply tubes to close and open the cardioplegia and blood supply tubes such that only one of the cardioplegia and blood supply tubes is open at a time, and a controller that controls the intervals during which the pinch valves are open to control the ratio of the cardioplegia medication and blood or blood substitute administered through the cardioplegia administration tube.

Abstract: A tubing set, the major portions of which are defined from a trilumen section of tubing, comprises three lines including an inflow line, an outflow line and a pressure sensing line. The inflow line includes a header portion engageable about the rollers of a peristaltic pump, a delivery tube mounting a male luer fitting for engagement with a patient cannula, and a pair of clampable supply tubes with bag spikes for communication with saline bags. The pressure sensing line includes a pressure transmitting elongate tubular diaphragm mounted within a fluid chamber directly adjacent the patient end of the line. The pressure transmitting diaphragm communicates with an elongate dry or air tube which, at the remote end, communicates with an appropriate pump-mounted pressure transducer or the like.

Abstract: A method and guide assembly for use in a tibial osteotomy wherein two pairs of parallel guide pins are inserted into the tibia at a predetermined angle with respect to each other through a guide block. The adjacent surfaces of the pairs of pins are then used to precisely guide a saw by which a wedge-shaped segment of the tibia is removed.