Abstract: A formulation for a culture medium that is specifically designed to supplement a culture of differentiating hematopoietic stem cells (HSCs) with the factors necessary for high density manufacture of red blood cells (RBCs) while only requiring partial medium exchanges on a periodic basis. Further, the present disclosure identifies a factor, iron (III) citrate, also referred to as ferric citrate, that can supplant more commonly utilized sources of biological iron in culture medium for a more stable, more cost-effective medium formulation for superior vertical scalability.

Abstract: An apparatus includes a transparent chamber having a space therein for containing an object while heating under vacuum, at least one directed energy source configured to direct energy to heat the object positioned within the space of the transparent chamber, a cap on the transparent chamber, and a connection between the transparent chamber and at least one vacuum for creating a vacuum within the transparent chamber. The apparatus may further include at least one temperature sensor to measure temperature of the object. The apparatus may further include a control system, the control system operatively connected to the at least one temperature sensor and the at least one directed energy source and wherein the control system is a closed loop system to adjust laser power to provide more or less energy to heat or maintain the temperature of the object.

Abstract: A method includes directing fluid flow from a bioreactor to an external path of a re-circulation loop, separating cells from the medium and returning the cells to the bioreactor thereby leaving spent medium, depleting the spent medium of biological waste products, replenishing the spent medium with beneficial growth factors to thereby provide replenished medium, and directing the replenished medium back to the bioreactor to thereby complete the re-circulation loop such that recycling of the medium occurs. The replenishing of the spent medium with beneficial growth factors may occur within a medium collection reservoir. The method may further include monitoring the spent medium within the medium collection reservoir including temperature of the spent medium within the medium collection reservoir and pH of the spent medium within the medium collection reservoir.

Abstract: A system includes a bioreactor having an inlet and an outlet, a cell-media recirculation loop between the outlet of the bioreactor and the inlet of the bioreactor, and a mature cell separation unit positioned along the cell-media recirculation loop wherein the mature cell separation unit is configured for performing separation of mature cells from immature cells and direction of the immature cells back to the inlet of the bioreactor. The system may further include a storage unit for storing the mature cells. The mature cell separation unit may be further configured to direct the mature cells to a mature cell storage unit.

Abstract: A modular system for manufacturing includes a modular housing, at least one manufacturing platform disposed within the modular housing for direct digital manufacturing of an item, and a modular grid for power and network connections positioned along a plurality of interior walls of the modular housing.

Abstract: A system includes a disposable patch having a first side and an opposite second side, the disposable patch having a body formed from a flexible material, a plurality of sensors positioned on the disposable patch, an electrically conductive pattern on the disposable patch wherein segments of the electrically conductive pattern are electrically connected to the plurality of sensors, and a reusable patch for positioning on the disposable patch, the reusable patch having a plurality of conductive connection points. The conductive pattern of the disposable patch and the conductive connection points of the reusable patch configured such that in an operative position the conductive connection points of the reusable patch are electrically connected to the conductive pattern such that one or more electrical components of the reusable patch are electrically connected to the plurality of sensors of the disposable patch through the conductive pattern.

Abstract: A method may include forming a cavity within a plastic structure with a channel positioned at a perimeter of the cavity, inserting the electronic component into the cavity, dispensing a dielectric fluid into the channel at the perimeter of the cavity, curing the dielectric fluid in situ to secure the electronic component within the cavity with a cured dielectric and printing interconnects for the electronic component.

Abstract: A bioreactor system having an expanding volume is provided. The system includes a bioreactor assembly comprising a plurality of bioreactors including at least a first bioreactor and a second bioreactor proximate the first bioreactor. The bioreactor system further includes a first gate between the first bioreactor and the second bioreactor such in a first configuration the gate is closed and the first bioreactor is isolated from the second bioreactor and in a second configuration the gate is open and the first bioreactor is contiguous with the second bioreactor and media and cells form a homogenous mixture within a combined volume formed by the first bioreactor in combination with the second bioreactor. The bioreactor system may further include a microdispenser for each of the plurality of bioreactors for controlling dispensement of media into each of the plurality of bioreactors.

Abstract: An assembly for performing an additive manufacturing process includes a first material feed for dispensing a first material, a second material feed for dispensing a second material, a material combiner chamber, a first entry channel fluidly connecting the first material feed and the material combiner chamber, and a second entry channel fluidly connecting the second material feed and the material combiner chamber. The assembly further includes a pen tip for dispensing a material in the additive manufacturing process, the material comprising the first material and the second material, a valve having a rod, a first seal between the material combiner and the pen tip, and a first actuator for moving the rod back and forth along a longitudinal axis to open and close the first seal.

Abstract: A method of additive manufacturing of an object includes directing laser energy from a laser to a region for material deposition, extruding material using an extruder at the region of material deposition, sensing temperature within the region of the material deposition, and electronically controlling the laser energy using the temperature so as to sufficiently heat the region for material deposition prior to extruding the material to increase strength of the object.

Abstract: A method of additive manufacturing of an object may include directing laser energy from a laser to a region for material deposition, extruding material using an extruder at the region of material deposition, sensing temperature within the region of the material deposition, and electronically controlling the laser energy using the temperature so as to sufficiently heat the region for material deposition prior to extruding the material to increase strength of the object. The method may include hardening or freezing extruded material through cooling in real-time.

Abstract: An apparatus includes a transparent chamber having a space therein for containing an object while heating under vacuum, at least one directed energy source configured to direct energy to heat the object positioned within the space of the transparent chamber, a cap on the transparent chamber, and a connection between the transparent chamber and at least one vacuum for creating a vacuum within the transparent chamber. The apparatus may further include at least one temperature sensor to measure temperature of the object. The apparatus may further include a control system, the control system operatively connected to the at least one temperature sensor and the at least one directed energy source and wherein the control system is a closed loop system to adjust laser power to provide more or less energy to heat or maintain the temperature of the object.

Abstract: An apparatus includes a substrate and an electronic circuit comprising a plurality of conductive tracts forming a printed litz line on the substrate for distributing a signal therebetween in order to increase effective conductance relative to a single conductive line not divided into tracts. The plurality of conductive tracts may be formed by printing a pattern on the substrate and removing portions of the pattern to leave the plurality of conductive tracts. The removing portions of the pattern may be performed by a removal process such as laser cutting, milling, etching, or masking. For example, the removal may be performed by applying ultrashort laser pulses. The printing may be performed by a jetting process, a spray process, an extrusion process, a dispensing process, and/or other types of processes for applying materials.

Abstract: A personalized device for therapeutic training includes a therapeutic device body, electronic circuitry integrated into the therapeutic device body wherein the electronic circuitry comprises at least one sensor. The therapeutic device body is personalized for use by a patient through sizing and shaping the therapeutic device based on measurements of the patient and 3D printing at least a portion of the therapeutic device body and at least a portion of the electronic circuitry to make the personalized device both electrically and mechanically functional.

Abstract: An apparatus for use in 3D fabrication includes a heat sink, a melt tube extending through the heat sink, the melt tube having a first end and an opposite second end and adapted for melting filament or other material as the material is conveyed from the first end to the second end, a pen tip having an opening therein for ejecting melted material, the pen tip at the second end of the melt tube, and a pen tip holder for securely holding the pen tip during printing, the pen tip holder having a heater element associated therewith.

Abstract: A reflector assembly includes a monolithic and adaptive reflector having a single and continuous reflecting surface and a focusing shape. The reflector assembly further includes a plurality of trusses for supporting the monolithic and adaptive reflector and a plurality of actuators, each of the plurality of actuators operatively connected to one of the plurality of trusses. Actuation of each of the plurality of actuators exerts a force which flexes at least a portion of the continuous reflecting surface.

Abstract: An apparatus includes a rigid frame or girder system, a first computer controlled motion system associated with the rigid frame or girder system and configured to move in coordinated positions, a second computer controlled motion system associated with a part to be worked on and configured to move in coordinated positions, and a plurality of sensors associated with the first motion system and the second motion system. The first computer controlled motion system and the second computer controlled motion system use information from the plurality of sensors to assist in coordination between the first computer controlled motion system and the second computer controlled motion system.

Abstract: A method of building a three dimensional (3D) structure includes micro-dispensing a layer comprising a material using a syringe-based micro-dispensing tool, curing the layer, and repeating the steps of micro-dispensing and curing a plurality of times in order to build the three-dimensional structure. The material may be loaded with nano to micron sized particles, tubes, or strings.

Abstract: A method of additive manufacturing of an object may include directing laser energy from a laser to a region for material deposition, extruding material using an extruder at the region of material deposition, sensing temperature within the region of the material deposition, and electronically controlling the laser energy using the temperature so as to sufficiently heat the region for material deposition prior to extruding the material to increase strength of the object. The method may include hardening or freezing extruded material through cooling in real-time.

Abstract: A method of additive manufacturing of an object includes directing laser energy from a laser to a region for material deposition, extruding material using an extruder at the region of material deposition, sensing temperature within the region of the material deposition, and electronically controlling the laser energy using the temperature so as to sufficiently heat the region for material deposition prior to extruding the material to increase strength of the object.