Publication number: 20220213492
Abstract: Described herein are enzymes, such as for example, methanol dehydrogenase (MDH), 3-hexulose-6-phosphate isomerase (PHI), 3-hexulose-6-phosphate synthase (HPS), ribose-5-phosphate isomerase (RPI), ribulose 5-phosphate 3-epimerase (RPE), transketolase (TKT), transaldolase (TAL) enzymes, phosphofructokinase (PFK), Sedoheptulose 1,7-Bisphosphatase (GLPX), fructose-bisphosphate aldolase (FBA), 6-phosphogluconate dehydrogenase (GND), and glucose-6-phosphate dehydrogenase (ZWF); recombinant host cells expressing the enzymes; methods of producing methylotrophic cells; and methods of producing amino acids (e.g., lysine).
Type:
Application
Filed:
April 17, 2020
Publication date:
July 7, 2022
Applicant:
Ginkgo Bioworks, Inc.
Inventors:
Hui Zhou, Massimo Merighi, Micjael G. Napolitano, Kennji Abe, Yoshihiro Ito, Takayuki Asahara, Thomas Perli, Sergio L. Florez, Ryan J. Putman, Ryo Takeshita, Yuri Uehara, Akito Chinen, Kazuteru Yamada