Patents by Inventor Kenzo Fujimoto
Kenzo Fujimoto has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230212178Abstract: In the present invention, a method for producing a compound of formula I comprises a step for causing a compound of formula III to undergo a Pechmann condensation reaction with respect to a compound of formula II in the presence of an organic solvent and an acid catalyst to obtain a compound of formula IV, and due to such method, provided are a novel photoreactive compound and a method for producing same that can be used for nucleic acid photoreaction technology.Type: ApplicationFiled: January 27, 2020Publication date: July 6, 2023Applicant: JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventor: Kenzo FUJIMOTO
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Patent number: 11225683Abstract: Provided are: a photocoupling method that overcomes the problem of the stagnation of photocoupling with a target nucleotide using a probe containing a photo-responsive nucleotide, and that improves the photocoupling efficiency; and a photocoupling kit. The photocoupling method is characterized by hybridizing a target site present in a nucleic acid sample with a first probe having a sequence complementary to the target site and containing a photo-responsive nucleotide, in a reaction solution, and carrying out photocoupling by photo-irradiation, wherein self-assembly caused by the photo-responsive nucleotide contained in the first probe is suppressed. The photocoupling kit is characterized by comprising a first probe having a sequence complementary to a target site present in a nucleic acid sample, and containing a photo-responsive nucleotide; and a second probe being highly complementary to the first probe.Type: GrantFiled: July 19, 2013Date of Patent: January 18, 2022Assignee: LSI MEDIENCE CORPORATIONInventors: Hirotake Wakamatsu, Akira Yanagihara, Hiroshi Terasaki, Nobutake Fugono, Mitsunobu Shimadzu, Kenzo Fujimoto
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Patent number: 11214590Abstract: Provided are a compound represented by formula I that can be used in nucleic acid photoreaction techniques, and a photoreactive crosslinking agent comprising the compound.Type: GrantFiled: July 25, 2018Date of Patent: January 4, 2022Assignees: JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, NICCA CHEMICAL CO., LTD.Inventors: Kenzo Fujimoto, Shigetaka Nakamura
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Patent number: 11078211Abstract: Provided are a compound represented by the following formula I; a novel photoreactive compound that can be used in nucleic acid photoreaction techniques by a photoreactive crosslinking agent comprising the compound; and a photoreactive crosslinking agent in which the photoreactive compound is used.Type: GrantFiled: July 19, 2018Date of Patent: August 3, 2021Assignees: JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, NICCA CHEMICAL CO., LTD.Inventors: Takahiro Inatsugi, Ayako Koto, Isao Ishimaru, Masahiko Takamura, Kenzo Fujimoto
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Patent number: 10844087Abstract: Provided is a means for preventing the inactivation of a photoresponsive nucleic acid probe by suppressing the formation of a photocrosslink between a modified nucleotide having a structure corresponding to the monomer of Formula (II) or an amino acid analogue of a nucleotide having a structure corresponding to the monomer of Formula (III) and a modified nucleotide having a structure corresponding to the monomer of Formula (I), wherein the modified nucleotide replaces at least one constituent nucleotide which is the photocrosslinkable 1-thyminyl or 1-uracilyl, by substituting at least one constituent nucleotide which is the photocrosslinkable 1-thyminyl or 1-uracilyl with a modified nucleotide having a structure corresponding to the monomer of Formula (I).Type: GrantFiled: October 30, 2014Date of Patent: November 24, 2020Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Kenzo Fujimoto, Shigetaka Nakamura
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Publication number: 20200317685Abstract: Provided are a compound represented by the following formula I; a novel photoreactive compound that can be used in nucleic acid photoreaction techniques by a photoreactive crosslinking agent comprising the compound; and a photoreactive crosslinking agent in which the photoreactive compound is used.Type: ApplicationFiled: July 19, 2018Publication date: October 8, 2020Applicants: JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY, NICCA CHEMICAL CO., LTD.Inventors: Takahiro Inatsugi, Ayako Koto, Isao Ishimaru, Masahiko Takamura, Kenzo Fujimoto
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Publication number: 20200172563Abstract: Provided are a compound represented by formula I that can be used in nucleic acid photoreaction techniques, and a photoreactive crosslinking agent comprising the compound.Type: ApplicationFiled: July 25, 2018Publication date: June 4, 2020Applicant: JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Kenzo Fujimoto, Shigetaka Nakamura
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Patent number: 10450334Abstract: The present invention provides a new photoreactive compound which can be used in technologies for photoreactions of nucleic acid, and also provides a photoreactive crosslinking agent comprising the above photoreactive compound. A photoreactive compound represented by the following formula I can be used.Type: GrantFiled: March 27, 2014Date of Patent: October 22, 2019Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Kenzo Fujimoto, Takashi Sakamoto, Yuya Tanaka
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Patent number: 9944979Abstract: Provided is a method for rapidly and easily detecting a mutated nucleic acid, which is contained in a small amount in a nucleic acid sample together with wild-type nucleic acids, with high specificity and high sensitivity. In the method of the present invention, amplification of a detection region comprising a target site by a nucleic acid amplification method is inhibited, by the steps of allowing a nucleic acid having a target site to coexist with a clamp probe comprising a photo-crosslinking nucleic acid and having a sequence complementary to the target site, and photo-crosslinking the nucleic acid having the target site with the clamp probe by photo-irradiation.Type: GrantFiled: September 9, 2011Date of Patent: April 17, 2018Assignee: LSI MEDIENCE CORPORATIONInventors: Hiroshi Terasaki, Tsunetada Konno, Mitsunobu Shimadzu, Kenzo Fujimoto, Takashi Sakamoto
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Patent number: 9771612Abstract: A method for detecting a target nucleic acid comprising: forming a three-component association product by allowing the association of at least a nucleic acid molecule, a first nucleic acid probe having a first marker bound thereto, and a second nucleic acid probe having a second marker bound thereto; forming at least one covalent bond between the target nucleic acid molecule and the first nucleic acid probe and between the target nucleic acid molecule and the second nucleic acid probe; and binding the three-component association product to a solid phase carrier through the second marker; recovering the three-component association product bound to the solid phase carrier; releasing the first marker from the recovered three-component association product; and detecting the target nucleic acid molecule by detecting the free first marker.Type: GrantFiled: September 15, 2014Date of Patent: September 26, 2017Assignee: OLYMPUS CORPORATIONInventors: Kunio Hori, Kenzo Fujimoto
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Publication number: 20160326207Abstract: Provided is a means for preventing the inactivation of a photoresponsive nucleic acid probe by suppressing the formation of a photocrosslink between a photoresponsive base having a photocrosslinkable vinyl structure and a photocrosslinkable thymine (T) or uracil (U) base, by substituting with an R group (R being —CN or —CO—R1, where R1 is a saturated or unsaturated straight-chain or branched cyclic or non-cyclic C1-12 hydrocarbon group) the 5 position of a pyrimidine ring of the thymine (T) or uracil (U) base which is photocrosslinkable with the photoresponsive base having a photocrosslinkable vinyl structure.Type: ApplicationFiled: October 30, 2014Publication date: November 10, 2016Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Kenzo Fujimoto, Shigetaka Nakamura
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Patent number: 9416403Abstract: A method of detecting a target nucleic acid by sandwich hybridization using a detection probe that hybridizes with the target nucleic acid, and a capture probe immobilized on a support wherein at least one nucleic acid base in the nucleic acid molecule(s) of the detection probe and/or capture probe with a photoreactive group includes irradiating, with light, a complex formed by hybridization between the target nucleic acid and the detection probe and/or capture probe to allow formation of a covalent bond(s) between the photoreactive group(s) and a nucleic acid base(s) in the target nucleic acid.Type: GrantFiled: August 29, 2013Date of Patent: August 16, 2016Assignee: Toray Industries, Inc.Inventors: Taisuke Hirano, Fumio Nakamura, Yoji Ueda, Kenzo Fujimoto
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Patent number: 9359393Abstract: The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology. The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent.Type: GrantFiled: December 26, 2013Date of Patent: June 7, 2016Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Kenzo Fujimoto, Masayuki Ogino, Yoshinaga Yoshimura
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Publication number: 20160031918Abstract: The present invention provides a new photoreactive compound which can be used in technologies for photoreactions of nucleic acid, and also provides a photoreactive crosslinking agent comprising the above photoreactive compound. A photoreactive compound represented by the following formula I can be used.Type: ApplicationFiled: March 27, 2014Publication date: February 4, 2016Applicant: JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Kenzo Fujimoto, Takashi Sakamoto, Yuya Tanaka
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Publication number: 20150240296Abstract: A method of detecting a target nucleic acid by sandwich hybridization using a detection probe that hybridizes with the target nucleic acid, and a capture probe immobilized on a support wherein at least one nucleic acid base in the nucleic acid molecule(s) of the detection probe and/or capture probe with a photoreactive group includes irradiating, with light, a complex formed by hybridization between the target nucleic acid and the detection probe and/or capture probe to allow formation of a covalent bond(s) between the photoreactive group(s) and a nucleic acid base(s) in the target nucleic acid.Type: ApplicationFiled: August 29, 2013Publication date: August 27, 2015Inventors: Taisuke Hirano, Fumio Nakamura, Yoji Ueda, Kenzo Fujimoto
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Publication number: 20150211057Abstract: Provided are: a photocoupling method that overcomes the problem of the stagnation of photocoupling with a target nucleotide using a probe containing a photo-responsive nucleotide, and that improves the photocoupling efficiency; and a photocoupling kit. The photocoupling method is characterized by hybridizing a target site present in a nucleic acid sample with a first probe having a sequence complementary to the target site and containing a photo-responsive nucleotide, in a reaction solution, and carrying out photocoupling by photo-irradiation, wherein self-assembly caused by the photo-responsive nucleotide contained in the first probe is suppressed. The photocoupling kit is characterized by comprising a first probe having a sequence complementary to a target site present in a nucleic acid sample, and containing a photo-responsive nucleotide; and a second probe being highly complementary to the first probe.Type: ApplicationFiled: July 19, 2013Publication date: July 30, 2015Applicant: LSI MEDIENCE CORPORATIONInventors: Hirotake Wakamatsu, Akira Yanagihara, Hiroshi Terasaki, Nobutake Fugono, Mitsunobu Shimadzu, Kenzo Fujimoto
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Publication number: 20150004607Abstract: A method for detecting a target nucleic acid comprising: forming a three-component association product by allowing the association of at least a nucleic acid molecule, a first nucleic acid probe having a first marker bound thereto, and a second nucleic acid probe having a second marker bound thereto; forming at least one covalent bond between the target nucleic acid molecule and the first nucleic acid probe and between the target nucleic acid molecule and the second nucleic acid probe; and binding the three-component association product to a solid phase carrier through the second marker; recovering the three-component association product bound to the solid phase carrier; releasing the first marker from the recovered three-component association product; and detecting the target nucleic acid molecule by detecting the free first marker.Type: ApplicationFiled: September 15, 2014Publication date: January 1, 2015Applicant: OLYMPUS CORPORATIONInventors: Kunio Hori, Kenzo Fujimoto
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Publication number: 20140107331Abstract: The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology. The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent.Type: ApplicationFiled: December 26, 2013Publication date: April 17, 2014Applicant: JAPAN SCIENCE AND TECHNOLOGY AGENCYInventors: Kenzo Fujimoto, Masayuki Ogino, Yoshinaga Yoshimura
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Patent number: 8697357Abstract: The present invention provides a method for detecting methylcytosine in DNA rapidly, conveniently, and with high sensitivity. The present invention relates to a method for detecting methylcytosine by using a methylcytosine photocoupling agent (a photoresponsive probe) consisting of nucleic acids having a group represented by the Formula (I), (II), (III) or (IV) as a base moiety.Type: GrantFiled: March 11, 2009Date of Patent: April 15, 2014Assignee: Japan Advanced Institute of Science and TechnologyInventors: Kenzo Fujimoto, Masayuki Ogino, Yuta Taya
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Patent number: 8642754Abstract: The present invention provides a manufacturing method that can easily manufacture a compound known as photoresponsive (photocoupling) nucleic acids at high yield in a shorter period of time than that of the conventional technology. The present invention relates to a method of manufacturing a photoresponsive nucleic acid which includes a step of reacting a nucleic acid having groups represented by the Formula I, the Formula III, the Formula IV, or the Formula V and a compound represented by the Formula II, or reacting a nucleic acid having groups represented by the Formula VI, the Formula VIII, the Formula IX, or the Formula X and a compound represented by the Formula VII by heating them by microwaves in the presence of a metal catalyst, a basic substance, and a solvent.Type: GrantFiled: February 13, 2009Date of Patent: February 4, 2014Assignee: Japan Science and Technology AgencyInventors: Kenzo Fujimoto, Masayuki Ogino, Yoshinaga Yoshimura