Abstract: Methods of reducing adapter-dimers in a sequencing library are provided according to aspects of the present disclosure, including: hybridizing excess 3? adapters to blocker oligonucleotides forming a hybridized complex, such that the excess 3? adapters are unavailable to form adapter-dimers, the hybridized complex including: a first blocker oligonucleotide 3? terminus adjacent to an adenylated nucleotide at the 5? terminus of a first unligated 3? adapter and a second blocker oligonucleotide 3? terminus adjacent to an adenylated nucleotide at the 5? terminus of a second unligated 3? adapter, wherein the first blocker oligonucleotide has a 5? portion complementary to and hybridized to the first unligated 3? adapter, the second blocker oligonucleotide has a 5? portion complementary to and hybridized to the second unligated 3? adapter, and the first blocker oligonucleotide has a 3? portion complementary to, and hybridized to, a 3? portion of the second blocker oligonucleotide.

Abstract: Methods, compositions, and kits of the present disclosure for normalizing a mass amount of nucleic acids in each of a plurality of test samples are provided, for use in methods of nucleic acid analysis, such that substantially equal amounts of nucleic acids are present in the samples to be analyzed.

Abstract: Sets of hybridization blockers, kits include at least one set of hybridization blockers, and methods of use thereof in massively parallel nucleic acid sequencing, are provided according to aspects of the present disclosure, each of the hybridization blockers comprising at least one Tm increasing nucleotide, the set of hybridization blockers for use in massively parallel sequencing of a plurality of nucleic acid sequencing library molecules, wherein the set of hybridization blockers efficiently blocks the complementary strand interactions between the adapter regions of different library molecules and is therefore effective to reduce the capture of non-target sequences during a capture enrichment hybridization to maximize the efficiency of the massively parallel sequencing techniques.

Abstract: Oligonucleotide primers and methods of use in producing sequencing libraries are provided according to aspects of the present disclosure which include, from 5? to 3?, a homopolymer-hybridizing region, and an anchor region comprising 5?-(?)nNm-3?, wherein the homopolymer-hybridizing region is a contiguous sequence of 5 to 20 elements, wherein the elements are nucleotides or Tm increasing nucleotide analogs, wherein at least 4 of the elements are Tm increasing nucleotide analogs, wherein the homopolymer-hybridizing region hybridizes to a complementary homopolymer tract of a target nucleic acid, wherein the complementary homopolymer tract comprises a contiguous sequence of complementary elements, wherein ? is any nucleotide or nucleotide analog with the proviso that ? is not a nucleotide or nucleotide analog complementary to a complementary element of the complementary homopolymer tract, and wherein N is any nucleotide or nucleotide analog.

Abstract: Disclosed are methods and compositions for amplification of genetic material, including isothermal WGA of single cells.

Abstract: Apparatus for fluorescent and ion sensing of DNA nucleotide incorporation events including DNA nucleotide incorporation structure designed to have sequencing primers bonded to a surface for the incorporation of DNA nucleotides thereon. At least some of the DNA nucleotides having a fluorescent label. A photodiode positioned adjacent the incorporation structure and an illumination device positioned adjacent the DNA nucleotide incorporation structure to illuminate DNA nucleotides incorporated onto the sequencing primers. The illumination device exciting the fluorescent labels when incorporation occurs and the photodiode positioned to sense the excited fluorescent labels. Ion sensing apparatus positioned adjacent the DNA nucleotide incorporation structure including a metal oxide thin film transistor with a gate electrically coupled to receive an electrical signal indicative of ion emissions produced by the DNA nucleotide incorporated onto DNA target fragments or sequencing primers.

Abstract: Disclosed are methods and compositions for amplification of genetic material, including isothermal WGA of single cells.

Abstract: Described herein are compositions and methods useful for the detection of nucleic acid variations. Ligation within a probe or between probes is used to distinguish between probes perfectly complementary to a target and those containing a mismatch. Nucleotide fill-in/extension steps are optionally applied according to the type of assay performed. A circularization and relinearization step can be applied to create a template for further amplification and detection. In certain aspects, portions of a target sequence or its complement are not amplified.