Abstract: Described herein are novel PRMT5 inhibitors of Formula I and pharmaceutically acceptable salts thereof, as well as the pharmaceutical compositions thereof. Compounds of the present invention are useful for inhibiting PRMT5 activity and may have use in treating proliferative, metabolic and blood disorders.

Abstract: A system for sterilizing an object including a hollow cassette for containing the object, a sealable opening in the cassette for ingress and egress of the object, a seal for forming a fluid-tight seal around the opening, input and output ports in the cassette for receiving and exhausting a sterilizing fluid and a sealing check valve in the input and output ports for providing a fluid-tight seal when no connections are made to the input and output ports such that when the object is sterilized within the cassette, the cassette will maintain a sterilized atmosphere for the object until the cassette is opened to allow use of the object.