Abstract: The disclosed embodiments concern methods for determining sequences of interest using targeted unique molecular index (TUMI) sequences that are uniquely associated with individual polynucleotide fragments in plants, such as those present in a transgenic event, a site-specific mutation or a wild type variant. System, apparatus, and computer program products are also provided for determining a sequence of interest implementing the methods disclosed.

Abstract: Methods for the high-throughput analysis of transgenic events are herein disclosed. The methods use libraries of sheared genomic DNA ligated to specialized adapters and pooled for sequence analysis and comparison to known genomic and insert sequence. The method finds use in detecting characterizing insertion site, transgene integrity, and transgene copy number.

Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Abstract: Methods for the high-throughput analysis of transgenic events are herein disclosed. The methods use libraries of sheared genomic DNA ligated to specialized adapters and pooled for sequence analysis and comparison to known genomic and insert sequence. The method finds use in detecting characterizing insertion site, transgene integrity, and transgene copy number.

Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Abstract: A method and system for automatically assigning employees to work plurality of cash registers when the employees report for work is described. The system and method automatically compares the employee's qualifications to the current operational status of a plurality of cash registers and any parameters that may govern the operation of the cash registers such as age requirements for the sale of alcohol. The system automatically assigns an employee to a cash register and transmits real time operational status of those cash registers to a supervisor via a wireless device.

Abstract: A method and system for automatically assigning employees to work plurality of cash registers when the employees report for work is described. The system and method automatically compares the employee's qualifications to the current operational status of a plurality of cash registers and any parameters that may govern the operation of the cash registers such as age requirements for the sale of alcohol. The system automatically assigns an employee to a cash register and transmits real time operational status of those cash registers to a supervisor via a wireless device.

Abstract: Methods for the high-throughput analysis of transgenic events are herein disclosed. The methods use libraries of sheared genomic DNA ligated to specialized adapters and pooled for sequence analysis and comparison to known genomic and insert sequence. The method finds use in detecting characterizing insertion site, transgene integrity, and transgene copy number.

Abstract: Methods for the high-throughput analysis of transgenic events are herein disclosed. The methods use libraries of sheared genomic DNA ligated to specialized adapters and pooled for sequence analysis and comparison to known genomic and insert sequence. The method finds use in detecting characterizing insertion site, transgene integrity, and transgene copy number.

Abstract: Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Abstract: Methods and compositions for maize target gene suppression and improving an agronomic trait through microRNAs or target gene modulation are disclosed. Polynucleotide constructs useful for gene silencing, or upregulation or modulation as well as cells, plants and seeds comprising the polynucleotides and methods for using microRNAs to silence a target gene are also described.

Abstract: The present disclosure provides methods to increase crop yield utilizing transgenic genes controlling plant growth and yield. The specific genes can be used to increase tissue growth and abiotic stress tolerance. Plants, plant progeny, seeds and tissues created by these methods are also described. Polynucleotides encoding the sequences are provided for expression in a plant of interest. Expression cassettes, plants, plant cells, plant parts and seeds comprising the sequences of the disclosure are further provided. In specific embodiments, the polynucleotide is operably linked to a constitutive promoter.

Abstract: Genetic male sterile plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production.

Abstract: The present invention provides methods and compositions of polypeptides having isoprene synthase activity with improved performance characteristics. In particular, the present invention provides legume isoprene synthases for increased isoprene production in recombinant host cells.

Abstract: Methods for the high-throughput analysis of transgenic events are herein disclosed. The methods use libraries of sheared genomic DNA ligated to specialized adapters and pooled for sequence analysis and comparison to known genomic and insert sequence. The method finds use in detecting characterizing insertion site, transgene integrity, and transgene copy number.

Abstract: A method and system for automatically assigning employees to work plurality of cash registers when the employees report for work is described. The system and method automatically compares the employee's qualifications to the current operational status of a plurality of cash registers and any parameters that may govern the operation of the cash registers such as age requirements for the sale of alcohol. The system automatically assigns an employee to a cash register and transmits real time operational status of those cash registers to a supervisor via a wireless device.

Abstract: The present disclosure provides polynucleotide sequences relating to the diurnal cycling in maize leaf and ear tissues. The disclosure provides polynucleotide sequences and the use of encoded polypeptides associated with the oscillation. The disclosed sequences are responsible for controlling plant growth, source-sink relationships and yield in crop plants.

Abstract: The present invention provides methods and compositions of polypeptides having isoprene synthase activity with improved performance characteristics. In particular, the present invention provides legume isoprene synthases for increased isoprene production in recombinant host cells.

Abstract: A field joint for connecting a plurality of downhole tool modules is disclosed. The modules include a housing and an electrical line. A bulkhead is coupled to a first module that includes a first conduit aperture for receiving an electrical connector assembly. The first electrical connector assembly is releasably coupled to the exterior portion of the first module and includes a first connector having a first end adapted for electrical coupling to an electrical line. A connector block is coupled to the second module that includes a second conduit aperture positioned to substantially face the first conduit aperture when the first and second modules are joined. A second electrical connector is disposed in the second conduit aperture and is electrically coupled to an electrical line such that an electrical contact is established with a second end of the first connector when the first and second modules are joined.

Abstract: A field joint for connecting a plurality of downhole tool modules is disclosed. The modules include a housing and an electrical line. A bulkhead is coupled to a first module that includes a first conduit aperture for receiving an electrical connector assembly. The first electrical connector assembly is releasably coupled to the exterior portion of the first module and includes a first connector having a first end adapted for electrical coupling to an electrical line. A connector block is coupled to the second module that includes a second conduit aperture positioned to substantially face the first conduit aperture when the first and second modules are joined. A second electrical connector is disposed in the second conduit aperture and is electrically coupled to an electrical line such that an electrical contact is established with a second end of the first connector when the first and second modules are joined.