Abstract: Processes for producing recombinant proteins having low levels of residual host cell proteins. The processes comprise utilizing engineered host cell lines in which specific host cell proteins are tagged with purification tags, wherein the purification tags can be used to remove those specific host cell proteins from the recombinant proteins.

Abstract: Mammalian cell lines genetically engineered to have reduced or eliminated expression of specific host cell proteins, and methods for using the engineered mammalian cell lines for the production of recombinant proteins having low levels of residual host cell protein contamination.

Abstract: The present invention provides mammalian cell lines that have been genetically engineered causing such cell lines to be resistant to viral entry and/or propagation, and provides methods of using said cell lines to reduce or prevent viral contamination of biologic production systems.

Abstract: The present invention provides cell lines deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1). Also provided are methods for producing the Mga1 deficient cell lines and methods for using the Mgat1 deficient cell lines for the production of recombinant proteins having simple glycoforms.

Abstract: The present invention provides mammalian cell lines that have been genetically engineered causing such cell lines to be resistant to viral entry and/or propagation, and provides methods of using said cell lines to reduce or prevent viral contamination of biologic production systems.

Abstract: The present invention provides cell lines deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1). Also provided are methods for producing the Mga1 deficient cell lines and methods for using the Mgat1 deficient cell lines for the production of recombinant proteins having simple glycoforms.

Abstract: The present invention provides cell lines deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1). Also provided are methods for producing the Mga1 deficient cell lines and methods for using the Mgat1 deficient cell lines for the production of recombinant proteins having simple glycoforms.

Abstract: The present disclosure encompasses an isolated cell comprising an exogenous nucleic acid sequence located within or proximal to a predetermined genomic locus, wherein the exogenous nucleic acid sequence comprises at least one recognition sequence which can be exploited by one or more polynucleotide modification enzymes for targeted integration of a recombinant protein. The disclosure further provides methods for preparing such cells, and methods for retargeting such cells for the production of recombinant proteins, and kits for the same.

Abstract: The present invention provides cell lines deficient in mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase I (Mgat1). Also provided are methods for producing the Mga1 deficient cell lines and methods for using the Mgat1 deficient cell lines for the production of recombinant proteins having simple glycoforms.

Abstract: The present invention provides methods, nucleic acid constructs, and kits for selectively deleting a region of a nucleic acid sequence. Specifically, it utilizes retargeting retroelements to place site-specific recombination sites at targeted locations in the nucleic acid sequence. The region between the recombination sites is then deleted using a site-specific recombination system.

Abstract: The invention provides an Enzyme Blend comprising a DNA polymerase and a DNA repair enzyme. Methods and kits for amplification of DNA that is damaged, undamaged, or suspected of being damaged are also provided.

Abstract: The present invention provides methods, nucleic acid constructs, and kits for selectively deleting a region of a nucleic acid sequence. Specifically, it utilizes retargeting retroelements to place site-specific recombination sites at targeted locations in the nucleic acid sequence. The region between the recombination sites is then deleted using a site-specific recombination system.

Abstract: The invention provides an Enzyme Blend comprising a DNA polymerase and a DNA repair enzyme. Methods and kits for amplification of DNA that is damaged, undamaged, or suspected of being damaged are also provided.

Abstract: A process for remediation of contaminated solid materials comprising polynuclear aromatic hydrocarbon contaminated solid materials, polychlorinated hydrocarbon contaminated materials, and mixtures thereof by sequential biological/chemical/biological treatment in which the contaminated solid materials are biodigested under suitable conditions by a first aerobic or anaerobic digestion, producing a first biodigestion product. The first biodigestion product is then contacted for chemical treatment with hydrogen peroxide in the presence of ferrous ion in amounts and under conditions suitable for chemical oxidation, forming a mixture and oxidizing the first biodigestion product, producing biodegradable hydrocarbon product materials having enhanced biodegradability. The product materials are then biodigested under suitable conditions by a second aerobic or anaerobic digestion.