Abstract: Microplate assemblies and methods for using same are provided. In accordance with some embodiments, microplate assemblies are provided, the assemblies comprising a microplate well portion that forms a microplate well and that includes at least one sensor in the microplate well that responds to analyte in the well. In accordance with some embodiments, methods for determining a cell consumption rate of an analyte are provided, the methods comprising: positioning a cell in a microplate well having at least one sensor located within the well; providing analyte to the microplate well at a controlled rate; detecting a response of the sensor to the analyte in the microplate well; and determining a cell consumption rate of the analyte based on the response of the sensor to the analyte.