Abstract: A disposable spinner flask includes an asymmetric container, a lid, and a spinning mechanism. The container can be sealingly coupled to the lid, which includes an access port and a cap configured to be coupled to the access port for preventing bacteria, viruses, and fungi from passing therethrough while allowing air to flow between the container and a surrounding environment. The lid may include a structure for retaining the cap when the cap is removed. The spinning mechanism can include a shaft, at least one blade, and a magnetic device. Further, the spinning mechanism can include a receptacle that fixedly receives the magnetic device toward an end of the blade assembly, the magnetic device configured to rotate when subjected to an external magnetic force.

Abstract: A lid for a well plate is provided. A pin penetrates the lid and projects downwardly into a well of the well plate. An electrical charge is applied to the pin and an electrical charge of opposite polarity is applied to an electrically conductive portion of a corresponding well, generating a small flow of electrical current and corresponding electrical fields within the well. Charged materials, such as DNA, selectably are attracted to the pin and segregated from the liquid sample.

Abstract: A disposable spinner flask includes an asymmetric container, a lid, and a spinning mechanism. The container can be sealingly coupled to the lid, which includes an access port and a cap configured to be coupled to the access port for preventing bacteria, viruses, and fungi from passing therethrough while allowing air to flow between the container and a surrounding environment. The lid may include a structure for retaining the cap when the cap is removed. The spinning mechanism can include a shaft, at least one blade, and a magnetic device. Further, the spinning mechanism can include a receptacle that fixedly receives the magnetic device toward an end of the blade assembly, the magnetic device configured to rotate when subjected to an external magnetic force.

Abstract: The present disclosure generally relates to a multi-well reservoir plate and methods of using the same. The multi-well reservoir plate includes a frame portion coupled to a plate portion, where a number of receptacles are formed in the plate portion. The receptacles or wells are sized to receive and support a substance without permitting the substance to leak or discharge from a passage formed in a lower portion of the well and in fluid communication with an upper portion of the well where the substances is supported. In one embodiment, the wells are at least partially filled with a substance, the substance is supported the in the wells for an amount of time, and then the substance is discharged from the wells by applying a force to the substance. By way of example, the force may be a pressure applied to an upper surface of the substance, a pressure (e.g., vacuum) applied to a lower surface of the substance, and/or an inertial force generated by an acceleration of the substance.

Abstract: A lid for a well plate, which has pins that penetrate the lid and project downwardly into the wells of the well plate. Thermal energy is applied to the upper end of the pin to regulate the temperature of liquid samples. Ultrasonic energy is applied to the upper end of the pin to sonicate the liquid sample. An electrical charge is applied to the upper end of the pin to attract charged materials, such as DNA, and to selectably segregate charged materials from the liquid sample.

Abstract: A lid for a well plate is provided. Pins penetrate the lid and project downwardly into the wells of the well plate. Thermal energy is applied to the upper end of the pin to regulate the temperature of liquid samples in the well plate. Ultrasonic energy is applied to the upper end of the pin to sonicate the liquid sample. An electrical charge is applied to the upper end of the pin to attract charged materials, such as DNA, and to selectably segregate charged materials from the liquid sample.

Abstract: A method of and apparatus for generating a multivariate spectra over a predetermined excitation wavelength region comprising directing light spanning the region at a sample, simultaneously measuring intensities of resulting fluorescence spectra as a function of a plurality of fluorescence wavelengths produced at one or more excitation wavelengths in the region, and producing a multivariate spectra from the intensities, fluorescence wavelengths, and excitation wavelengths.

Abstract: A lid for a well plate is provided. Pins penetrate the lid and project downwardly into the wells of the well plate. Thermal energy is applied to the upper end of the pin to regulate the temperature of liquid samples in the well plate. Ultrasonic energy is applied to the upper end of the pin to sonicate the liquid sample. An electrical charge is applied to the upper end of the pin to attract charged materials, such as DNA, and to selectably segregate charged materials from the liquid sample.

Abstract: A thermal cycling device consisting of a plate containing a plurality of wells to hold liquid and a lid that covers the plate and contains a number of “pins” that insert into the wells of the plate to control the temperature of the sample in the well. Biological, chemical or other samples are placed into the wells of the plate and the lid is placed on the plate with the pins inserted into or in the proximity of the sample. The lid, the outer surface of which may be copper clad and in direct contract with the pins, is heated or cooled, rapidly and uniformly heating or cooling the biological samples. The temperature of the sample can be rapidly and uniformly cycled and is particularly useful for the amplification of DNA via the polymerase chain reaction.

Abstract: Lid for a well plate having wells therein having pin-like members which project downwardly into the wells of said well plate. The invention has means and pins associated with each means for causing the pins to change temperature or vibrate ultrasonically or control the polarity and magnitude of an electrical charge placed on the pins. The means can, respectively, alter the temperature of a fluid contained in the wells or sever material contained in the liquid or attract or repel particles suspended in the liquid.

Abstract: Method of making an electrotransport device containing an analog of a parent polypeptide having one or more amino acid residues substituted relative to the parent polypeptide with an amino acid residue selected from the group consisting of proline, glycine and asparagine.

Abstract: Methods for modifying polypeptide agents to enhance their transdermal electrotransport flux are provided. The polypeptide is modified by reducing the potential of the polypeptide for forming &agr;-helical or &bgr;-sheet segments. In particular, amino acid residues known to stabilize &agr;-helical and &bgr;-sheet segments can be replaced with destabilizing residues and known helix breakers. Modified molecules and compositions including the molecules are also provided.

Abstract: An apparatus for holding liquid, such as a microtiter plate, in which a plurality of wells are formed. Each of the wells have side walls that intersect in an edge that defines the boundary between adjacent wells. The edge boundary prevents liquid from collecting between the wells, thereby causing all of the liquid applied to the plate to enter one of the wells. The microtiter plate can be formed from a material having a reflectance and/or fluorescence that is optimized for the particular application in which the microtiter plate will be used. Microtiter plates for use in fluorescence measurement applications are made from material having low fluorescence, such as a liquid crystal polymer, while microtiter plates for use in luminescence measurements are made from material having a high reflectance. Microtiter plates for spectrophotometric applications are made with wells having clear bottoms and opaque walls.

Abstract: PTH analogs comprising an amino acid sequence that is: SVSEIQLLHNX.sub.1 X.sub.2 X.sub.3 HX.sub.4 X.sub.3 X.sub.3 X.sub.3 X.sub.5 RVX.sub.5 WLR X.sub.4 X.sub.4 LX.sub.3 X.sub.3 VX.sub.1 X.sub.3 X.sub.3 X (SEQ ID NO:10) wherein X.sub.1 is a neutral or positively charged amino acid, X.sub.2 is a neutral amino acid, X.sub.3 is a neutral, positively charged, or negatively charged amino acid, X.sub.4 is a positively charged amino acid, X.sub.5 is a positively charged or negatively charged amino acid, and X is selected from the group consisting of Hol, Ho, a homoserine amide, or the sequence of amino acids comprising residues 35-84 of PTH, have enhanced activity and increased serum half-life as compared with human PTH. The PTH analogs can be produced as fusion proteins in high yields in E. coli host cells; the fusion proteins can be subsequently cleaved to produce the PTH analog.

Abstract: Methods for modifying polypeptide agents to enhance their transdermal electrotransport flux are provided. The polypeptide is modified by reducing the potential of the polypeptide for forming .alpha.-helical or .beta.-sheet segments. In particular, amino acid residues known to stabilize .alpha.-helical and .beta.-sheet segments can be replaced with destabilizing residues and known helix breakers. Modified molecules and compositions including the molecules are also provided.

Abstract: The present invention relates to methods of delivering pharmaceutical agents across membranes, including the skin layer or mucosal membranes of a patient. A pharmaceutical agent is covalently bonded to a chemical modifier, via a physiologically cleavable bond, such that the membrane transport and delivery of the agent is enhanced.