Abstract: The present application discloses a magnetic microchip having a graph code as well as a preparation method and application thereof. The magnetic microchip comprises: a graph code comprising more than one opaque microstructure mainly consisting of colloid aggregates formed by aggregating magnetic solid particles, wherein adjacent magnetic solid particles are isolated at least by an organic molecule and/or an inorganic molecule; and a transparent encapsulating structure at least used for wrapping the opaque microstructure. The magnetic microchip of the present application is prepared by adopting a micromachining process and has high in machining precision and repeatability, the size difference between different batches or between different individuals at the same batch can be ignored, use is convenient, and accuracy of a detection result can be effectively ensured, thereby greatly improving analysis quality.

Abstract: The disclosure discloses an encoded chip based micro-array and a preparation method thereof. In a typical embodiment, the preparation method comprises: providing a carrier, which has at least one fluid accommodating cavity, wherein at least one carrying surface is distributed in the fluid accommodating cavity; uniformly coating the carrying surface with an adhesive; adding a selected fluid to the fluid accommodating cavity till the carrying surface is immersed by the selected fluid; depositing encoded microchips dispersed in the selected fluid on the carrying surface, and enabling the encoded microchips to be combined with the adhesive distributed on the carrying surface; and curing the adhesive, thereby fixing the microchips onto the carrying surface. The disclosure further discloses a biochemical kit based on the micro-array, a biochemical detection system and method, and the like.

Abstract: The present application discloses a magnetic microchip having a graph code as well as a preparation method and application thereof. The magnetic microchip comprises: a graph code comprising more than one opaque microstructure mainly consisting of colloid aggregates formed by aggregating magnetic solid particles, wherein adjacent magnetic solid particles are isolated at least by an organic molecule and/or an inorganic molecule; and a transparent encapsulating structure at least used for wrapping the opaque microstructure. The magnetic microchip of the present application is prepared by adopting a micromachining process and has high in machining precision and repeatability, the size difference between different batches or between different individuals at the same batch can be ignored, use is convenient, and accuracy of a detection result can be effectively ensured, thereby greatly improving analysis quality.

Abstract: A micro-liquid phase reaction method based on a substrate with a hydrophilic-hydrophobic patterned surface, including the following: applying a liquid phase system containing a hydrotropic substance and/or an amphipathic substance to a hydrophobic smooth plane in a sample-spotting manner to form an array of tiny droplets, subsequently removing the solvent in each droplet to bond the hydrotropic substance and/or amphipathic substance in each droplet to the hydrophobic smooth plane so as to form an array of hydrophilic bonding points, then moving an aqueous phase system or hydrophilic liquid phase system containing more than one reactants over the hydrophobic smooth plane, thereby forming island-like tiny reaction droplets at each hydrophilic bonding point, and finally under the set reaction conditions, reacting the reactants in each tiny reaction droplet.

Abstract: A micro-liquid phase reaction method based on a substrate with a hydrophilic-hydrophobic patterned surface, including the following: applying a liquid phase system containing a hydrotropic substance and/or an amphipathic substance to a hydrophobic smooth plane in a sample-spotting manner to form an array of tiny droplets, subsequently removing the solvent in each droplet to bond the hydrotropic substance and/or amphipathic substance in each droplet to the hydrophobic smooth plane so as to form an array of hydrophilic bonding points, then moving an aqueous phase system or hydrophilic liquid phase system containing more than one reactants over the hydrophobic smooth plane, thereby forming island-like tiny reaction droplets at each hydrophilic bonding point, and finally under the set reaction conditions, reacting the reactants in each tiny reaction droplet.

Abstract: The present invention provides a kit and a detection method for multiple targets detection of biomolecules. The kit comprises a universal tag, a probe and an optional instruction for using the same. The universal tag in the present invention is a fragment of DNA, RNA, peptide nucleic acid, or LNA, and is 3-20 mer in length. The probe in the present invention contains in order from 3? terminus to 5? terminus, a nucleotide sequence which is reverse complementary to a target molecule or a portion of the target molecule, and a nucleotide sequence which is reverse complementary to the universal tag; or said probe contains in order from 3? terminus to 5? terminus, a nucleotide sequence which is reverse complementary to the universal tag, and a nucleotide sequence which is reverse complementary to a target molecule or a portion of the target molecule.

Abstract: The present invention provides universal tags, probes and detection methods for multiple targets detection of biomolecules. The universal tag in the present invention is a fragment of DNA, RNA, peptide nucleic acid, or LNA, and is 3-20 mer in length. The probe in the present invention contains in order from 3? terminus to 5? terminus, a nucleotide sequence which is reverse complementary to a target molecule or a portion of the target molecule, and a nucleotide sequence which is reverse complementary to the universal tag; or said probe contains in order from 3? terminus to 5? terminus, a nucleotide sequence which is reverse complementary to the universal tag, and a nucleotide sequence which is reverse complementary to a target molecule or a portion of the target molecule.