Abstract: A methodology is provided for assaying the ability of a peptide of interest to displace an endogenously loaded peptide in a recombinantly produced, secreted, individual, soluble HLA trimolecular complex.

Abstract: The present invention relates generally to a methodology for assaying the binding of a peptide to an individual, specific, soluble HLA molecule.

Abstract: The field of the invention relates in general to at least one method and apparatus for the production of soluble MHC antigens and more particularly, but not by way of limitation, to at least one method and apparatus for the production of soluble Class I and II HLA molecules. The field of the invention also includes such produced soluble Class I and II HLA molecules and their use. According to the methodology of the present invention, the soluble Class I and II HLA molecules can be produced from either gDNA or cDNA starting material.

Abstract: The field of the invention relates in general to at least one method and apparatus for the production of soluble MHC antigens and more particularly, but not by way of limitation, to at least one method and apparatus for the production of soluble Class I and II HLA molecules. The field of the invention also includes such produced soluble Class I and II HLA molecules and their use. According to the methodology of the present invention, the soluble Class I and II HLA molecules can be produced from either gDNA or cDNA starting material.

Abstract: The field of the invention relates in general to at least one method and apparatus for the production of soluble MHC antigens and more particularly, but not by way of limitation, to at least one method and apparatus for the production of soluble Class I and II HLA molecules. The field of the invention also includes such produced soluble Class I and II HLA molecules and their use. According to the methodology of the present invention, the soluble Class I and II HLA molecules can be produced from either gDNA or cDNA starting material.

Abstract: The present invention relates generally to a methodology for assaying the binding of a peptide to an individual, specific, soluble HLA molecule. The peptides utilized in the method may be identified by indirect methods utilizing T lymphocytes, or by a direct method of epitope discovery described herein.

Abstract: The present invention generally relates to a MHC ligand database populated with MHC ligand sequences, motifs, extended motifs, submotifs, ligands unique to infected cells, tumor specific ligands, as well as a collection of current and future developed MHC ligand sequences developed by alternative methods. Other than the ligand sequences developed by alternative methods (which are in many cases non-standardized), the remaining ligand sequences are obtained in a standardized and minimum-variable dependent manner from soluble HLA molecules constructed according to the methodology described herein. The present invention further includes methodologies incorporating linear and predictive algorithm searching and comparison utilities.