Patents by Inventor Kilian R. Knoll

Kilian R. Knoll has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods and compositions for using argonaute to modify a single stranded target nucleic acid
    Patent number: 10253311
    Abstract: The present disclosure provides compositions, kits, genetically modified cells, non-human transgenic organisms, and methods for binding and/or cleaving a single stranded target nucleic acid. A method of cleaving includes contacting a single stranded target nucleic acid with (e.g., introducing into a cell) a subject argonaute (Ago) polypeptide and a guide RNA (e.g., having a 5?-OH). In some embodiments, a subject Ago polypeptide includes an amino acid sequence having 70% or more sequence identity with amino acids 282-430 and/or 431-639 of the Marinitoga piezophila argonaute (MpAgo) protein set forth in SEQ ID NO: 1. The present disclosure provides variant Ago polypeptides; and methods of use of same.
    Type: Grant
    Filed: April 9, 2015
    Date of Patent: April 9, 2019
    Assignee: The Regents of the University of California
    Inventors: Jennifer A. Doudna, Emine Kaya, Kilian R. Knoll
  • METHODS AND COMPOSITIONS FOR USING ARGONAUTE TO MODIFY A SINGLE STRANDED TARGET NUCLEIC ACID
    Publication number: 20170175104
    Abstract: The present disclosure provides compositions, kits, genetically modified cells, non-human transgenic organisms, and methods for binding and/or cleaving a single stranded target nucleic acid. A method of cleaving includes contacting a single stranded target nucleic acid with (e.g., introducing into a cell) a subject argonaute (Ago) polypeptide and a guide RNA (e.g., having a 5?-OH). In some embodiments, a subject Ago polypeptide includes an amino acid sequence having 70% or more sequence identity with amino acids 282-430 and/or 431-639 of the Marinitoga piezophila argonaute (MpAgo) protein set forth in SEQ ID NO: 1. The present disclosure provides variant Ago polypeptides; and methods of use of same.
    Type: Application
    Filed: April 9, 2015
    Publication date: June 22, 2017
    Inventors: Jennifer A. Doudna, Emine Kaya, Kilian R. Knoll