Patents by Inventor Kimberly M. Brown

Kimberly M. Brown has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Microbial Trypsin Mutants having Chymotrypsin Activity And Nucleic Acids Encoding Same
    Publication number: 20120142075
    Abstract: The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.
    Type: Application
    Filed: February 13, 2012
    Publication date: June 7, 2012
    Applicant: Novozymes, Inc.
    Inventors: Alan Klotz, Kimberly M. Brown, Elizabeth J. Zaretsky
  • Methods for obtaining microbial trypsin mutants having chymotrypsin activity
    Patent number: 8114635
    Abstract: The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, 193, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.
    Type: Grant
    Filed: February 18, 2010
    Date of Patent: February 14, 2012
    Assignee: Novozymes, Inc.
    Inventors: Alan Klotz, Kimberly M. Brown, Elizabeth J. Zaretsky
  • Microbial trypsin mutants having chymotrypsin activity and nucleic acids encoding same
    Publication number: 20100151515
    Abstract: The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193A, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.
    Type: Application
    Filed: February 18, 2010
    Publication date: June 17, 2010
    Applicant: Novozymes, Inc.
    Inventors: Alan Klotz, Kimberly M. Brown, Elizabeth J. Zaretsky
  • Microbial trypsin mutants having chymotrypsin activity and nucleic acids encoding same
    Patent number: 7705137
    Abstract: The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193A, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.
    Type: Grant
    Filed: January 30, 2008
    Date of Patent: April 27, 2010
    Assignee: Novozymes, Inc.
    Inventors: Alan Klotz, Kimberly M. Brown, Elizabeth J. Zaretsky
  • Microbial Trypsin Mutants Having Chymotrypsin Activity And Nucleic Acids Encoding Same
    Publication number: 20090035820
    Abstract: The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, S193A, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.
    Type: Application
    Filed: January 30, 2008
    Publication date: February 5, 2009
    Applicant: Novozymes, Inc.
    Inventors: Alan Klotz, Kimberly M. Brown, Elizabeth J. Zaretsky
  • Microbial trypsin mutants having chymotrypsin activity and nucleic acids encoding same
    Patent number: 7364892
    Abstract: The present invention relates to microbial trypsin variants having chymotrypsin-like activity, comprising: (a) a one or more substitutions corresponding to positions 144, 193, 198, 201, 218, 223, 227, 228, 229, 230, and 231 of amino acids 25 to 248 of SEQ ID NO: 2, (b) one or more deletions corresponding to positions 192, 197, and 226 of amino acids 25 to 248 of SEQ ID NO: 2; and (c) an insertion between positions corresponding to positions 224 and 225 of amino acids 25 to 248 of SEQ ID NO: 2. The present invention further relates to nucleotide sequences encoding microbial trypsin variants having chymotrypsin-like activity; nucleic acid constructs, expression vectors, and recombinant host cells comprising such nucleotide sequences; and methods of producing microbial trypsin variants having chymotrypsin-like activity or a precursor thereof.
    Type: Grant
    Filed: September 24, 2003
    Date of Patent: April 29, 2008
    Assignee: Novozymes, Inc.
    Inventors: Alan Klotz, Kimberly M. Brown, Elizabeth J. Zaretsky
  • Polypeptides having phospholipase B activity and nucleic acids encoding same
    Patent number: 7091003
    Abstract: The present invention relates to isolated polypeptides having phospholipase B activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: July 13, 2000
    Date of Patent: August 15, 2006
    Assignee: Novozymes, Inc.
    Inventors: Paul Harris, Kimberly M. Brown
  • Nucleic acids encoding polypeptides having cellobiose dehydrogenase activity
    Patent number: 6280976
    Abstract: The present invention relates to isolated nucleic acid sequences encoding polypeptides having cellobiose dehydrogenase activity. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as recombinant methods for producing the polypeptides.
    Type: Grant
    Filed: January 5, 2000
    Date of Patent: August 28, 2001
    Assignee: Novozymes Biotech, Inc.
    Inventors: Elizabeth J. Golightly, Kimberly M. Brown
  • Carboxypeptidases and nucleic acids encoding the same
    Patent number: 6187578
    Abstract: The present invention relates to polypeptides having carboxypeptidase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to methods of obtaining protein hydrolysates useful as flavor improving agents.
    Type: Grant
    Filed: October 3, 1997
    Date of Patent: February 13, 2001
    Assignees: Novo Nordisk Biotech, Inc., Novo Nordisk A/S
    Inventors: Alexander Blinkovsky, Randy Berka, Michael Rey, Elizabeth Golightly, Alan Klotz, Thomas Erik Mathisen, Claus Dambmann, Kimberly M. Brown
  • Polypeptides having pectin acetylesterase activity and nucleic acids encoding same
    Patent number: 6184028
    Abstract: The present invention relates to isolated polypeptides having pectin acetylesterase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: August 26, 1999
    Date of Patent: February 6, 2001
    Assignee: Novo Nordisk Biotech, Inc.
    Inventors: Michael D. Thomas, Kimberly M. Brown
  • Polypeptides having phospholipase B activity and nucleic acids encoding same
    Patent number: 6146869
    Abstract: The present invention relates to isolated polypeptides having phospholipase B activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing and using the polypeptides.
    Type: Grant
    Filed: October 21, 1999
    Date of Patent: November 14, 2000
    Assignee: Novo Nordisk Biotech, Inc.
    Inventors: Paul Harris, Kimberly M. Brown