Abstract: Ambulatory monitors and related methods employ an adhesive layer with conductive and non-conductive regions to improve electrical isolation of electrodes. An ambulatory monitor includes a biocompatible layer, first and second electrodes, an adhesive layer, and a physiological data recording module. The adhesive layer is interfaced with the biocompatible layer and configured to adhere the biocompatible layer to the user's skin. The adhesive layer has a first conductive adhesive region comprising a conductive adhesive electrically coupled with the first electrode, a second conductive adhesive region comprising a conductive adhesive electrically coupled with the second electrode, and a non-conductive adhesive region comprising a non-conductive adhesive. The non-conductive adhesive region serves as an electrical insulator between the first conductive adhesive region and the second conductive adhesive region.