Patents by Inventor Kimikazu Hashino
Kimikazu Hashino has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 7790849Abstract: An isolated polypeptide includes the amino acid sequence shown in SEQ. ID No. 13 and an isolated nucleic acid encoding the polypeptide, such as an isolated nucleic acid including the nucleic acid sequence shown in SEQ. ID No. 17. The isolated polypeptide includes two heparin binding polypeptides connected in tandem and the isolated nucleic acid encodes these. The polypeptide and nucleic acid sequences can improve retroviral vector mediated gene transfer efficiency into target cells.Type: GrantFiled: September 30, 2008Date of Patent: September 7, 2010Assignee: Takara Bio Inc.Inventors: Kiyozo Asada, Takashi Uemori, Takashi Ueno, Nobuto Koyama, Kimikazu Hashino, Ikunoshin Kato
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Publication number: 20090209730Abstract: A polypeptide represented by SEQ. ID No. 13 and a gene encoding the polypeptide. A gene is also represented by SEQ. ID No. 17, or a gene hybridizable thereto under stringent conditions and encoding a polypeptide which improves the efficiency of gene transfer into target cells with a retrovirus.Type: ApplicationFiled: September 30, 2008Publication date: August 20, 2009Inventors: Kiyozo Asada, Takashi Uemori, Takashi Ueno, Nobuto Koyama, Kimikazu Hashino, Ikunoshin Kato
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Patent number: 7446170Abstract: A polypeptide represented by SEQ. ID No. 13, a polypeptide represented by SEQ. ID No. 30 or functional equivalents thereof and a polypeptide represented by SEQ. ID No. 17.Type: GrantFiled: July 14, 2005Date of Patent: November 4, 2008Assignee: Takara Bio Inc.Inventors: Kiyozo Asada, Takashi Uemori, Takashi Ueno, Nobuto Koyama, Kimikazu Hashino, Ikunoshin Kato
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Publication number: 20080113451Abstract: A relatively simple and easy method of laveling in which a sample used in electrophoresis technology is labeled with the use of rare earth fluorescent complex DTBTA-Eu3+ as a labeling agent so as to exhibit a fluorescence intensity being stable without dependence on the intensity of electric field. Further, there can be realized an analysis of biosubstance with high sensitivity utilizing the complex.Type: ApplicationFiled: August 17, 2005Publication date: May 15, 2008Inventors: Kazuko Matsumoto, Yoshinori Yamaguchi, Kimikazu Hashino
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Publication number: 20080044903Abstract: Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferring receptor, or introducing a new functional group into the functional substance.Type: ApplicationFiled: July 27, 2007Publication date: February 21, 2008Applicants: Takara Shuzo Co., Ltd., TAKARA BIO INC.Inventors: Mitsuhiro UENO, Hirofumi Yoshioka, Haruko Konishi, Kimikazu Hashino, Mio Morishita, Hideto Chono, Tsuyoshi Miyamura, Mutsumi Sano, Kiyozo Asada, Kei Fujinaga, Ikunoshin Kato
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Publication number: 20060166924Abstract: Gene therapeutics to be used in treating diseases showing sensitivity to gene therapy, characterized by containing as the active ingredient an efficacious amount of a functional substance which has a function of having an affinity for a virus containing a gene usable in the gene therapy and another function of having an affinity specific for a target cell with a need for the gene transfer, or an efficacious amount of a functional substance which has an affinity for the above virus and an efficacious amount of another functional substance which has an affinity specific for the above cell.Type: ApplicationFiled: March 28, 2006Publication date: July 27, 2006Applicant: TAKARA BIO INC.Inventors: Ikunoshin Kato, Kiyozo Asada, Mitsuhiro Ueno, Kimikazu Hashino, Hirofumi Yoshioka, Keiji Tanaka
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Publication number: 20060030046Abstract: A polypeptide represented by SEQ. ID No. 13, a polypeptide represented by SEQ. ID No. 30 or functional equivalents thereof and a polypeptide represented by SEQ. ID No. 17.Type: ApplicationFiled: July 14, 2005Publication date: February 9, 2006Applicant: Takara Bio Inc., a corporation of JapanInventors: Kiyozo Asada, Takashi Uemori, Takashi Ueno, Nobuto Koyama, Kimikazu Hashino, Ikunoshin Kato
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Patent number: 6949623Abstract: The present invention provides a method for increasing the efficiency of gene transfer into target cells with a retrovirus. The transduction is affected by infecting target cells with a retrovirus in the presence of a mixture of a functional material having a retrovirus binding domain, and a second functional material having target cell binding domain. The target cells may be selected from the group of unintentional hematopoietic progenitor cells and erythrocyte precursor, specifically pluripotent stem cells or embryopalstic stem cells.Type: GrantFiled: February 2, 2001Date of Patent: September 27, 2005Assignee: Takara Bio Inc.Inventors: Kiyozo Asada, Takashi Uemori, Takashi Ueno, Nobuto Koyama, Kimikazu Hashino, Ikunoshin Kato
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Patent number: 6787359Abstract: Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferrin receptor, or introducing a new functional group into the functional substance.Type: GrantFiled: January 9, 2001Date of Patent: September 7, 2004Assignee: Takara Shuzo Co., Ltd.Inventors: Mitsuhiro Ueno, Hirofumi Yoshioka, Haruko Konishi, Kimikazu Hashino, Mio Morishita, Hideto Chono, Tsuyoshi Miyamura, Mutsumi Sano, Kiyozo Asada, Kei Fujinaga, Ikunoshin Kato
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Publication number: 20040058447Abstract: Improved methods for transferring a gene into target cells by using a retrovirus, wherein the gene transfer efficiency is improved and the target cells are efficiently transformed by binding the retrovirus to a functional substance which is immobilized on as carrier and having an activity of binding to retroviruses followed by washing; using an antibody capable of specifically recognizing cells, laminin or mannose-rich type sugar chain as a substance having an activity of binding to the target cells; pre-treating the target cells so as to inactivate transferrin receptor, or introducing a new functional group into the functional substance.Type: ApplicationFiled: September 9, 2003Publication date: March 25, 2004Applicant: Takara Shuzo Co., Ltd.Inventors: Mitsuhiro Ueno, Hirofumi Yoshioka, Haruko Konishi, Kimikazu Hashino, Mio Morishita, Hideto Chono, Tsuyoshi Miyamura, Mutsumi Sano, Kiyozo Asada, Kei Fujinaga, Ikunoshin Kato
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Publication number: 20030087437Abstract: The present invention provides a method for increasing the efficiency of gene transfer into target cells with a retrovirus. The transduction is effected by infecting target cells with a retrovirus in the presence of a mixture of a functional material having a retrovirus binding domain, and a second functional material having target cell binding domain. The target cells may be selected from the group of unipotential hematopoietic progenitor cells and erythrocyte precursor, specifically pluripotent stem cells or embryopalstic stem cells.Type: ApplicationFiled: February 2, 2001Publication date: May 8, 2003Inventors: Kiyozo Asada, Takashi Uemori, Takashi Ueno, Nobuto Koyama, Kimikazu Hashino, Ikunoshin Kato
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Patent number: 6426042Abstract: The present invention provides a kit to carry out retrovirus-mediated gene transfer into target cells. The kit contains a functional material bearing a retrovirus binding domain, another functional material bearing a target cell binding domain, an artificial substrate for incubating the retrovirus contacted with the target cells, and a target cell growth factor for pre-stimulating target cells to spur them along the cell cycle. The kit of the invention may further comprise a recombinant retroviral vector, necessary buffers, and the like.Type: GrantFiled: August 2, 1999Date of Patent: July 30, 2002Assignee: Takara Shuzo Co., Ltd.Inventors: Kiyozo Asada, Takashi Uemori, Takashi Ueno, Nobuto Koyama, Kimikazu Hashino, Ikunoshin Kato
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Patent number: 6194192Abstract: The present invention offers a method for the purification or removal of virus characterized in containing a step where the virus in the virus-containing sample is adsorbed with sulfated-fucose-containing polysaccharide(s) and/or degradation product(s) thereof.Type: GrantFiled: July 23, 1998Date of Patent: February 27, 2001Assignee: Takara Shuzo Co., Ltd.Inventors: Takashi Ueno, Kimikazu Hashino, Kiyozo Asada, Ikunoshin Kato
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Patent number: 5962326Abstract: An isolated SDS-resistant hyperthermostable .beta.-galactosidase gene derived from Pyrococcus furiosus. Examples thereof include genes respectively shown in SEQ ID NO: 1 and SEQ ID NO: 2 and genes each hybridizable with the above gene shown in SEQ ID NO: 1. A method of cloning the hyperthermostable .beta.-galactosidase gene in which each of the above genes or pacts thereof is used as a probe or primer. A process for producing a hyperthermostable .beta.-galactosidase by culturing a transformant into which a plasmid containing each of the above gene has been introduced.Type: GrantFiled: December 18, 1997Date of Patent: October 5, 1999Inventors: Atsushi Shimada, Miki Odate, Nobuto Koyama, Kimikazu Hashino, Kiyozo Asada, Ikunoshin Kato
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Patent number: 5824547Abstract: A method is disclosed for the efficient production of a transfected cell which comprises a step of culturing transfected cells in the presence of a cell-adhesive substance, after injection of a foreign gene into target cells, upon production of the transfected cells by transfer of a foreign gene into the target cells through cell perforation. Also provided are transfected cells produced by the method, and a kit for the production of transfected cells that includes a cell-adhesive substance as an essential component, which kit is suitable for use with the method for the efficient production of transfected cells by cell perforation.Type: GrantFiled: May 22, 1997Date of Patent: October 20, 1998Assignee: Takara Shuzo Co., Ltd.Inventors: Kimikazu Hashino, Hideyuki Matsushita, Ikunoshin Kato
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Patent number: 5744345Abstract: An isolated hyperthermostable .beta.-galactosidase gene derived from Pyrococcus furiosus is described. Examples include DNA sequences shown in SEQ ID NO. 2 and SEQ ID NO. 4 and DNAs hybridizable with these DNAs. A method of cloning the hyperthermostable .beta.-galactosidase gene in which each of the above sequences or portions thereof is used as a probe or primer is described. A process for producing a hyperthermostable .beta.-galactosidase by culturing a transformant into which a plasmid containing each of the above genes has been introduced is described. A particularly preferred gene encodes an SDS-resistant hyperthermostable .beta.-galactosidase.Type: GrantFiled: June 14, 1995Date of Patent: April 28, 1998Assignee: Takara Shuzo Co., Ltd.Inventors: Atsushi Shimada, Miki Odate, Nobuto Koyama, Kimikazu Hashino, Kiyozo Asada, Ikunoshin Kato
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Patent number: 5475100Abstract: This invention provides an antibody having antigen binding activity and which has a heavy chain constant region into which has been introduced an RGDS amino acid sequence giving the antibody affinity for cells, including macrophages. The antibody exhibits artificial cell adhesive activity which is the newly expressed activity that results from insertion of the RGDS amino acid sequence. This antibody having artificial cell adhesive activity can accelerate the phagocytosis of macrophages, and can activate other effector cells. Therefore, this antibody contributes to the self-defense mechanism.Type: GrantFiled: August 19, 1993Date of Patent: December 12, 1995Assignees: Fujita Health University, Takara Shuzo Co., Ltd.Inventors: Kimikazu Hashino, Fusao Kimizuka, Ikunoshin Kato, Yoshikazu Kurosawa, Koiti Titani, Kiyotoshi Sekiguchi
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Patent number: 5136023Abstract: A polypeptide having the cell-spreading activity of human fibronectin. Methods of preparing the polypeptide are described.Type: GrantFiled: November 16, 1989Date of Patent: August 4, 1992Assignee: Takara Shuzo Co., Ltd.Inventors: Kimikazu Hashino, Shouichi Goto, Yasutoshi Kawase, Yoh'ichi Ohdate, Yuki Taguchi, Tatsuru Kinoshita, Fusao Kimizuka, Ikunoshin Kato