Abstract: Disclosed are methods and devices for rapid parallel molecular affinity assays performed in a microfluidic environment. The invention exploits hydrodynamic addressing to provide simultaneous performance of multiple assays in parallel using a minimal sample volume flowing through a single channel.

Abstract: Disclosed are methods and devices for rapid parallel molecular affinity assays performed in a microfluidic environment. The invention exploits hydrodynamic addressing to provide simultaneous performance of multiple assays in parallel using a minimal sample volume flowing through a single channel.

Abstract: Disclosed are methods and devices for rapid parallel molecular affinity assays performed in a microfluidic environment. The invention exploits hydrodynamic addressing to provide simultaneous performance of multiple assays in parallel using a minimal sample volume flowing through a single channel.

Abstract: Disclosed is a microfluidic assay system and methods that apply flow injection analysis to permit dispersion monitoring. A solution containing a reagent that binds an analyte and a tracer is delivered via pressure-driven flow into the receiving end of the injection channel of the system of the invention. A sample fluid suspected of containing the analyte is delivered into the upstream end of the input channel under conditions permitting flow of the sample fluid toward the downstream end of the assay channel and permitting dispersion of the reagent into the sample fluid. The amount of tracer present in the fluid as it passes over the reference region and the capture region and the amount of binding between the analyte and the capture region are detected. The amount of binding detected between the analyte and the capture region is correlated to the amount of tracer detected in the reference region.

Abstract: Disclosed are methods and devices for rapid parallel molecular affinity assays performed in a microfluidic environment. The invention exploits hydrodynamic addressing to provide simultaneous performance of multiple assays in parallel using a minimal sample volume flowing through a single channel.

Abstract: Disclosed is a microfluidic assay system and methods that apply flow injection analysis to permit dispersion monitoring. A solution containing a reagent that binds an analyte and a tracer is delivered via pressure-driven flow into the receiving end of the injection channel of the system of the invention. A sample fluid suspected of containing the analyte is delivered into the upstream end of the input channel under conditions permitting flow of the sample fluid toward the downstream end of the assay channel and permitting dispersion of the reagent into the sample fluid. The amount of tracer present in the fluid as it passes over the reference region and the capture region and the amount of binding between the analyte and the capture region are detected. The amount of binding detected between the analyte and the capture region is correlated to the amount of tracer detected in the reference region.

Abstract: Streptavidin molecules are disclosed that contain a secondary functional domain. In preferred embodiments, the secondary domain is a cell adhesion peptide incorporated in the streptavidin amino acid sequence at a site not interfering with biotin binding. In a preferred embodiment, the cell adhesion peptide is arginine-glycine-aspartate (Arg-Gly-Asp) (RGD). The peptide is preferably placed on an exposed loop of the streptavidin molecule, such as within the loop defined by residues 63 to 69. The mutant streptavidin molecule can have other characteristics such as reduced biotin binding due to a modification of an amino acid at the biotin binding site. Preferred uses for the disclosed streptavidin molecules are as adaptors to bring, via a streptavidin/biotin interaction, the secondary functional domain into proximity with a cell or molecule to be affected and as a coating for substrates such as vascular devices or prostheses.