Patents by Inventor Klaus Beaucamp
Klaus Beaucamp has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 5061625Abstract: To prepare a microorganism producing .alpha.-galactosidase, not only a DNA containing an .alpha.-galactosidase gene but also a vector which is appropriate to the transformable cells to be used and contains antibiotic resistance genes are completely split with restriction endonuclease Sal I, the fragment of approximately four megadaltons of relative molecular weight is obtained from the fragments of the DNA containing the .alpha.-galactosidase gene, is mixed with the solution of the vector also split with Sal I, and is recombined in the presence of DNA ligase with the formation of a recombinant DNA.Type: GrantFiled: August 24, 1988Date of Patent: October 29, 1991Assignee: Boehringer Mannheim GmbHInventors: Ralf Mattes, Klaus Beaucamp
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Patent number: 4861717Abstract: The present invention provides a micro-organism of the species Escherichia coli or Pseudomonas putida, wherein it constitutively forms creatinamidinohydrolase.The present invention also provides a process for the production of such a micro-organism.Type: GrantFiled: January 6, 1986Date of Patent: August 29, 1989Assignee: Boehringer Mannheim GmbHInventors: Gunter Schumacher, Peter Buckel, Klaus Beaucamp
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Patent number: 4397952Abstract: The present invention provides a process for obtaining glucose dehydrogenase by culturing a micro-organism in an appropriate nutrient medium and obtaining the enzyme from the cell mass or from the culture medium, wherein the micro-organism cultured is Bacillus cereus DSM 1644. The nutrient medium used contains a carbon source which consists predominantly or solely of glycerol.Type: GrantFiled: May 4, 1981Date of Patent: August 9, 1983Assignee: Boehringer Mannheim GmbHInventors: Detlef von Hoerschelmann, Hans Seidel, Gerhard Berger, Armin Masuth, Klaus Beaucamp, Wolfgang Gruber
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Patent number: 4360596Abstract: The present invention provides a process for obtaining cholesterol esterase from micro-organisms of the genus Pseudomonas, wherein Pseudomonas sp. DSM 1280 or DSM 1281 is cultured in an appropriate culture medium in the presence of an inducer and the enzyme is obtained from the culture medium and/or from the cells.Type: GrantFiled: August 6, 1980Date of Patent: November 23, 1982Assignee: Boehringer Mannheim GmbHInventors: Klaus Beaucamp, Michael Nelboeck, Helmgard Gauhl, Hans Seidel, Wolfgang Gruber, Herwig Brunner
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Patent number: 4343903Abstract: The present invention provides a process for obtaining cholesterol esterase from micro-organisms, wherein a micro-organism capable of cholesterol esterase formation is cultured in an appropriate nutrient medium in the presence of lecithin as inducer and the enzyme is obtained from the culture medium and/or from the cells.Type: GrantFiled: August 6, 1980Date of Patent: August 10, 1982Assignee: Boehringer Mannheim GmbHInventors: Klaus Beaucamp, Michael Nelboeck, Helmgard Gauhl, Hans Seidel, Wolfgang Gruber, Herwig Brunner
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Patent number: 4334023Abstract: The present invention provides a process for obtaining cholesterol oxidase, wherein Streptomyces griseofuscus DSM 40191, Streptomyces hygroscopicus 40771, Streptomyces acidomyceticus DSM 40798 and/or Arthrobacter paraffinens DSM 312 are cultured and the enzyme obtained from the culture supernatant and/or the cells.Type: GrantFiled: May 7, 1980Date of Patent: June 8, 1982Assignee: Boehringer Mannheim GmbHInventors: Helmgard Gauhl, Georg Schawohl, Hans Seidel, Klaus Beaucamp
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Patent number: 4301028Abstract: A control reagent for heparin activity determination comprising20 to 1500 USP heparin,2000 to 30000 IU antithrombin III,0.15 to 1.5 millimoles serum albumin,0.005 to 0.05 mole ethylenediaminetetraacetic acid sodium salt,0 to 0.4 mole saccharose,2000 to 20000 U aprotinin, andbuffer substance, pH 6.5 to 7.5,per liter of control reagent solution.Type: GrantFiled: January 9, 1980Date of Patent: November 17, 1981Assignee: Boehringer Mannheim GmbHInventors: Knut Bartl, Joachim Ziegenhorn, Peter Wunderwald, Klaus Beaucamp, Helmut Lill
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Patent number: 4212938Abstract: Cholesterol is determined by incubating a sample suspected of containing cholesterol in an aqueous medium with cholesterol oxidase and determining either the oxygen consumption, the hydrogen peroxide formed, or cholestenone, as a measure of the initial cholesterol content; reagent compositions comprising cholesterol oxidase and a system for the determination of H.sub.2 O.sub.2 or for the determination of cholestenone are provided.Type: GrantFiled: May 15, 1973Date of Patent: July 15, 1980Assignee: Boehringer Mannheim GmbHInventors: Wolfgang Gruber, Hans U. Bergmeyer, Erich Bernt, Alexander Hagen, Peter Roeschlau, Gunter Lang, Klaus Beaucamp
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Patent number: 4186052Abstract: A novel peroxidase for reduced nicotinamide-adenine-dinucleotide is provided which peroxidase oxidizes reduced nicotinamide-adenine-dinucleotide with hydrogen peroxide to give nicotinamide-adenine-dinucleotide and water and is characterized by a Michaelis constant K.sub.Mto hydrogen peroxide of 2.8.times.10.sup.-5 M andto reduced nicotinamide-adenine-dinucleotide of 1.7.times.10.sup.-5 M,measured at 25.degree. C. in 0.2M tris buffer of pH 6.0, containing 0.1M potassium acetate. The peroxidase can be prepared by liberating the enzyme from Streptococcus faecalis ATCC 8043 by digestion or by treatment with a surface-active agent and isolating same from the enzyme solution obtained. Hydrogen peroxide is determined in a simple reaction or in a coupled reaction with a specific oxidase by contacting the said peroxidase with the H.sub.2 O.sub.2 producing reaction mixture at a pH of from 6.0 to 9.0 and measuring the change of extinction as a measure of H.sub.2 O.sub.Type: GrantFiled: June 29, 1977Date of Patent: January 29, 1980Assignee: Boehringer Mannheim GmbHInventors: Albert Roder, Hans Mollering, Wolfgang Gruber, Klaus Beaucamp, Hans Seidel, Peter Stahl, Detlef von Hoerschelmann
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Patent number: 4164448Abstract: Cholesterol oxidase free of unstabilizing residual traces of detergent is activated by adding to the cholesterol oxidase, before use thereof, at least one surface-active compound with lipophilic and hydrophilic properties. Diagnostic agents for determining cholesterol comprising cholesterol oxidase, a hydrogen peroxide detection system, a buffer, and at least one surface-active compound having lipophilic and hydrophilic properties are provided.Type: GrantFiled: February 14, 1977Date of Patent: August 14, 1979Assignee: Boehringer Mannheim GmbHInventors: Peter Roeschlau, Gunter Lang, Klaus Beaucamp, Erich Bernt, Wolfgang Gruber
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Patent number: 4144129Abstract: A new enzyme, cholesteroloxidase, is prepared by recovering same from microorganisms, preferably by washing a cholesterol-converting microorganism with butanol/water, disintegrating the microorganism culture and extracting same with a buffer solution containing a non-ionic surface-active agent, and isolating cholesterol oxidase from the extract.Type: GrantFiled: May 15, 1973Date of Patent: March 13, 1979Assignee: Boehringer Mannheim GmbHInventors: Wolfgang Gruber, Hans U. Bergmeyer, Michael Nelbock-Hochstetter, Klaus Beaucamp, Gunter Holz, Johanna Gramsall, Gunter Lang
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Patent number: 4080262Abstract: Enzymes, particularly labile enzymes, and most particularly microorganism derived labile enzymes, are stabilized by adding thereto a stabilizing agent comprising a protein fraction obtained by extracting yeast with water at 80.degree. to 100.degree. C., mixing the clear extract with 5 - 20% wt./vol. trichloracetic acid and separating the precipitate obtained.Type: GrantFiled: January 26, 1977Date of Patent: March 21, 1978Assignee: Boehringer Mannheim GmbHInventors: Klaus Beaucamp, Helmut Lill
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Patent number: 4008127Abstract: Cholesterol oxidase which oxidizes cholesterol with oxygen to give cholestenone and hydrogen peroxide, is prepared by culturing Nocardia erthropolis ATCC 17895, Nocardia erthropolis ATCC 4277, Nocardia formica ATCC 14811 or Proactinomyce erythropolis NCIB 9158 first on a peptone-containing mineral salt medium and thereafter on at least one sterol compound as the sole source of carbon, and then isolating the cholesterol oxidase formed from the cultured micro-organism, wherein said sterol compound is of the formula ##STR1## in which R.sub.1 and R.sub.2 are hydrogen or together represent a double bond,R.sub.3 and R.sub.4 are hydrogen or together represent a double bond, andR.sub.5 is hydrogen or alkyl containing up to 3 carbon atoms.Type: GrantFiled: November 20, 1975Date of Patent: February 15, 1977Assignee: Boehringer Mannheim G.m.b.H.Inventors: Wolfgang Gruber, Hans Ulrich Bergmeyer, Michael Nelboeck-Hochstetter, Klaus Beaucamp, Gunter Holz, Johanna Gramsall, Gunter Lang
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Patent number: 3969287Abstract: Carrier-bound proteins are prepared by reacting a protein in aqueous solution with a coupling compound having at least one group capable of acylating or alkylating proteins and at least one additional group capable of producing a bond with a carrier material, and reacting the resulting product with a carrier material, optionally forming the carrier material in situ by polymerization of a monomer or monomer mixture in the presence of said product.Type: GrantFiled: November 30, 1973Date of Patent: July 13, 1976Assignee: Boehringer Mannheim GmbHInventors: Dieter Jaworek, Michael Nelbock-Hochstetter, Klaus Beaucamp, Hans Ulrich Bergmeyer, Karl-Heinz Botsch
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Patent number: 3951744Abstract: NADP-dependent dehydrogenases are separated and purified by subjecting mixtures containing it to affinity chromatography using as the affinity chromatography material a nucleotide with a phosphate group in the 2'- or 3'-position, bound to a solid carrier.Type: GrantFiled: July 16, 1974Date of Patent: April 20, 1976Assignee: Boehringer Mannheim G.m.b.H.Inventors: Albert Roder, Klaus Beaucamp, Gunter Weimann, Walter Schneider, Klaus Muhlegger
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Patent number: 3948728Abstract: The new enzyme steroid-.DELTA.-isomerase is provided, as well as a process for producing same, comprising digesting a micro-organism containing said isomerase, extracting same by destruction of the cell wall with a buffer solution containing a non-ionic surfactant, centrifuging the resulting mass and discarding the precipitate, applying the extract to an anion exchanger, eluting the enzyme with a buffer solution containing a non-ionic surfactant, heating the eluate, separating the precipitate and isolating the enzyme therefrom from the supernatant.Type: GrantFiled: April 9, 1975Date of Patent: April 6, 1976Assignee: Boehringer Mannheim G.m.b.H.Inventors: Peter Roeschlau, Gunter Lang, Klaus Beaucamp, Erich Bernt