Abstract: A tissue cutting device is described, a representative one of which includes: a handle part; a head unit including comprising a plurality of blades arranged so as to extend substantially parallel with a longitudinal direction of the handle part at intervals and a blade holding portion for holding the plurality of blades; and a head holding part located at the distal end of the handle part, for holding the head.

Abstract: A probe set is described that includes a first probe having a target-binding substance and a base sequence X1, a second probe having a base sequence X2c and a base sequence X1c hybridizable with the base sequence X1, and a third probe having a base sequence X2 hybridizable with the base sequence X2c. A method for detecting a target substance is also described.

Abstract: A method of detecting a gene as an amplified product includes subjecting a solution containing the gene to a gene amplification reaction at a predetermined reaction temperature in the presence of a surfactant having a higher clouding point than the predetermined reaction temperature and detecting the gene as an amplified product, and a reagent kit for the method of detecting the gene as the amplified product utilizing the gene amplification includes a primer necessary for amplifying the gene, an enzyme for amplifying the gene, and a surfactant having a clouding point higher than the predetermined reaction temperature, wherein the primer is packed separately from the enzyme, and the surfactant is packed with the primer or the enzyme, or separately.

Abstract: In detecting a nucleic acid by using the nucleic acid amplification method, it is intended to shorten the time required for the measurement so as to systematically and efficiently examine a gene, etc. To achieve this object, use is made of a method of directly amplifying a nucleic acid by treating a collected biological sample wherein a means of inhibiting the degradation activity of the nucleic acid is introduced during or after the step of homogenizing the biological sample and thus the nucleic acid is directly amplified without isolating/purifying the nucleic acid component from the biological sample. In this method, the means of inhibiting the degradation activity of the nucleic acid is introduced at an acidic pH value (more specifically, from pH 2.5 to pH 5) with the use of a salt interacting with a substance inhibiting the nucleic acid amplification reaction and/or the nucleic acid component so as to directly amplify the nucleic acid without isolating/purifying the nucleic acid component.

Abstract: A method of detecting PS2V characterized by comprising reacting PS2V in a sample with a primary antibody which is bonded to a solid phase and specifically recognizes PA2V, then reacting with a secondary antibody recognizing PS2 or PS2V by any of the following procedures: (a) reacting with a secondary antibody having been enzyme-labeled and recognizing PS2 or PS2V; (b) reacting with a secondary antibody having been biotinylated and recognizing PS2 or PS2V and then reacting with an avidinylated or streptoavidinylated enzyme; (c) reacting with a secondary antibody having been biotinylated and recognizing PS2 or PS2V and then reacting with a biotinylated enzyme and avidin or streptoavidin; and (d) reacting with a secondary antibody recognizing PS2 or PS2V and then reacting with an antibody having been enzyme-labeled and recognizing the secondary antibody; then adding the substrate of the above enzyme and detecting the product formed by the enzyme reaction.

Abstract: A method of detecting a gene as an amplified product includes subjecting a solution containing the gene to a gene amplification reaction at a predetermined reaction temperature in the presence of a surfactant having a higher clouding point than the predetermined reaction temperature and detecting the gene as an amplified product, and a reagent kit for the method of detecting the gene as the amplified product utilizing the gene amplification includes a primer necessary for amplifying the gene, an enzyme for amplifying the gene, and a surfactant having a clouding point higher than the predetermined reaction temperature, wherein the primer is packed separately from the enzyme, and the surfactant is packed with the primer or the enzyme, or separately.

Abstract: A method for testing causative bacterial species of food poisoning which is characterized by using two oligonucleotide primers that hybridize to opposite strands of bacterial DNA specifically, and flank a unique region in the target DNA and amplifying the specific fragment of the bacterial DNA, comprising the steps of:(a) hybridizing the primer to specific gene sequence of bacteria in a sample, extending the hybridized primer with deoxynucleotide triphosphates (dATP, dCTP, dGTP, and dTTP), and resultantly making the double strand nucleotide;(b) where the primer extension products are cleaved into each single strand of nucleotide by certain external force such as heat, pH and so on, one single strand functioning as a template for nucleotide extension with a primer of the other strand;(c) repeating a series of cycles involving cleavage of primer extension products, primer hybridizing, extension of the hybridized primers to amplify the specific fragment of DNA, and detecting the amplified DNA fragment; and(d) as

Abstract: Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.

Abstract: Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherchia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.

Abstract: Oligonucleotides (SEQ ID NOs 1-8) selectively hybridizable with a specific gene of Vibro parahaemolyticus, oligonucleotides (SEQ ID NOs 9-13) selectively hybridizable with the LT gene of toxigenic Escherichia coil, oligonucleotides (SEQ ID NOs 14-21) selectively hybrizable with the STh or STp gene of toxigenic Escherichia coil, oligonucleotides (SEQ ID NOs 22-47) selectively hybridizable with the entA, B, C, or D gene of Staphylococcus aureus, or oligonucleotides (SEQ ID NOs 48-53) selectively hybridizable with the entE gene of Staplyloccus aureus are prepared and used as primers for gene amplification to thereby selectively detect only respective microorganisms causing food poisoning.

Abstract: The method of capillary electrophoresis of the present invention is carried out using an electrophoretic buffer containing an ungelled agarose polymer, wherein the agarose polymer concentration may be constant or may be changed on a time basis.The method of the present invention permits efficient fractional assay with high reproductibility. Particularly, the mode using the apparatus of the present invention in which the agarose polymer concentration is changed on a time basis is suitable for fractional assay of DNA and proteins of a wide range of size because an electrophoretic buffer having an ideal composition for separation is supplied constantly.