Patents by Inventor Koji Kimata
Koji Kimata has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8137941Abstract: A chondroitin polymerase having such properties that it transfers GlcUA and GalNAc alternately to a non-reduced terminal of a sugar chain from a GlcUA donor and a GalNAc donor, respectively, and the like; and a process for producing the chondroitin polymerase.Type: GrantFiled: August 10, 2009Date of Patent: March 20, 2012Assignee: Seikagaku CorporationInventors: Toshio Ninomiya, Nobuo Sugiura, Koji Kimata
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Patent number: 8129148Abstract: Provided are a method for producing a fraction containing more than 50% of CH represented by the general formula (1), which comprises at least the step of allowing a glucuronic acid donor, an N-acetylgalactosamine donor, a saccharide receptor, a chondroitin polymerase derived from the Escherichia coli K4 strain, and Mn2+ at a final concentration of 0.02 to 100 mM to coexist, and performing a reaction thereof under conditions of 20 to 40° C. and pH 6 to 8 for 0.5 minutes to 4 hours, and a method for producing a fraction containing more than 50% of CH represented by the general formula (2), which comprises at least the step of performing the reaction under same conditions for 10 hours or longer, which enable industrial scale production of a CH fraction of a controlled even number saccharide and odd number saccharide content ratio by a simple procedure at a low cost.Type: GrantFiled: August 23, 2006Date of Patent: March 6, 2012Assignee: Seikagaku CorporationInventors: Nobuo Sugiura, Koji Kimata
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Patent number: 8067204Abstract: A method for producing a chondroitin sugar chain comprises reacting a glucuronic acid donor, an N-acetyl galactosamine donor, a sugar receptor and a bacterial cell enzyme which synthesizes chondroitin in the presence of a surfactant. The surfactant is selected from polyoxyethylene octadecyl amine, n-decanoyl-N-methylglucamide, sodium cholate, n-octyl-?-D-thioglucopyranoside, n-nonyl-?-D-thiomaltopyranoside, sucrose monocholate, sucrose monocaprate, and sucrose monolaurate. The chondroitin sugar chain has all the following properties: a weight average molecular weight: 50,000 or more when measured by gel filtration chromatography; it is completely degraded to disaccharides with chondroitinase ABC; and when the sugar chain is decomposed with chondroitinase ABC and the decomposed products are subjected to a disaccharide analysis, substantially all of them correspond to an unsaturated disaccharide unit of chondroitin.Type: GrantFiled: December 14, 2006Date of Patent: November 29, 2011Assignee: Seikagaku CorporationInventors: Nobuo Sugiura, Satoshi Shimokata, Koji Kimata
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Patent number: 8026090Abstract: Disclosed are: (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, or (B) a polypeptide comprising an amino acid sequence of SEQ ID NO:2 including deletion, substitution or addition of one or several amino acid residues and having chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce the polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal.Type: GrantFiled: March 11, 2011Date of Patent: September 27, 2011Assignee: Seikagaku CorporationInventors: Yoshimitsu Kakuta, Takuo Osawa, Nobuo Sugiura, Koji Kimata
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Publication number: 20110195476Abstract: Disclosed are: (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, or (B) a polypeptide comprising an amino acid sequence of SEQ ID NO:2 including deletion, substitution or addition of one or several amino acid residues and having chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce the polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal.Type: ApplicationFiled: March 11, 2011Publication date: August 11, 2011Applicant: Seikagaku CorporationInventors: Yoshimitsu KAKUTA, Takuo Osawa, Nobuo Sugiura, Koji Kimata
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Patent number: 7939065Abstract: The invention provides a chondroitin sulfate synthesis promoter useful for the treatment of diseases such as articular disease and discopathy. The chondroitin sulfate synthesis promoter contains, as an active ingredient, chondroitin sulfate glucuronyltransferase protein and/or chondroitin sulfate N-acetylgalactosaminyltransferase-1 protein, or a gene encoding the enzyme protein(s).Type: GrantFiled: November 18, 2008Date of Patent: May 10, 2011Assignees: Tokyo Medical and Dental University, Seikagaku CorporationInventors: Kenichiro Sakai, Koji Kimata, Kenichi Shinomiya, Hideto Watanabe
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Patent number: 7927837Abstract: Disclosed are: (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, or (B) a polypeptide comprising an amino acid sequence of SEQ ID NO:2 including deletion, substitution or addition of one or several amino acid residues and having chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce the polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal.Type: GrantFiled: June 12, 2007Date of Patent: April 19, 2011Assignee: Seikagaku CorporationInventors: Yoshimitsu Kakuta, Takuo Osawa, Nobuo Sugiura, Koji Kimata
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Patent number: 7723092Abstract: A chondroitin polymerase having such properties that it transfers GlcUA and GalNAc alternately to a non-reduced terminal of a sugar chain from a GlcUA donor and a GalNAc donor, respectively, and the like; and a process for producing the chondroitin polymerase.Type: GrantFiled: July 12, 2006Date of Patent: May 25, 2010Assignee: Seikagaku CorporationInventors: Toshio Ninomiya, Nobuo Sugiura, Koji Kimata
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Publication number: 20100068759Abstract: Disclosed are: (A) a polypeptide consisting of the amino acid sequence of SEQ ID NO:2, or (B) a polypeptide comprising an amino acid sequence of SEQ ID NO:2 including deletion, substitution or addition of one or several amino acid residues and having chondroitin synthase activity; a nucleic acid encoding the polypeptide; a method for producing the polypeptide, comprising at least the steps of: (1) expressing the nucleic acid to produce the polypeptide; and (2) collecting the polypeptide produced in the step (1); and a crystal of the polypeptide. The crystal may be a monoclinic or tetragonal crystal.Type: ApplicationFiled: June 12, 2007Publication date: March 18, 2010Applicant: Seikagaku CorporationInventors: Yoshimitsu Kakuta, Takuo Osawa, Nobuo Sugiura, Koji Kimata
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Publication number: 20090305357Abstract: A chondroitin polymerase having such properties that it transfers GlcUA and GalNAc alternately to a non-reduced terminal of a sugar chain from a GlcUA donor and a GalNAc donor, respectively, and the like; and a process for producing the chondroitin polymerase.Type: ApplicationFiled: August 10, 2009Publication date: December 10, 2009Applicant: Seikagaku Kogyo Kabushiki KaishaInventors: Toshio NINOMIYA, Nobuo Sugiura, Koji Kimata
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Publication number: 20090263867Abstract: A method for producing a chondroitin sugar chain comprises at least the following step: a step of allowing “a glucuronic acid donor”, “an N-acetyl galactosamine donor”, “a sugar receptor” and “a bacterial cell enzyme which synthesizes chondroitin” to coexist in a reaction system in the presence of a surfactant. Here, the surfactant is preferably selected from n-nonyl-?-D-thiomaltopyranoside, sucrose monocaproate and sucrose monolaurate. The chondroitin sugar chain has all the following properties 1) to 3): 1) a weight average molecular weight: 50,000 or more when it is measured by gel filtration chromatography, 2) it is completely degraded to disaccharides with chondroitinase ABC, 3) when the sugar chain is decomposed with chondroitinase ABC and the decomposed products are subjected to a disaccharide analysis, substantially all of them correspond to an unsaturated disaccharide unit of chondroitin.Type: ApplicationFiled: December 14, 2006Publication date: October 22, 2009Applicant: Seikagaku CorporationInventors: Nobuo Sugiura, Satoshi Shimokata, Koji Kimata
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Patent number: 7598068Abstract: A chondroitin polymerase having such properties that it transfers GlcUA and GalNAc alternately to a non-reduced terminal of a sugar chain from a GlcUA donor and a GalNAc donor, respectively, and the like; and a process for producing the chondroitin polymerase.Type: GrantFiled: August 12, 2002Date of Patent: October 6, 2009Assignee: Seikagaku CorporationInventors: Toshio Ninomiya, Nobuo Sugiura, Koji Kimata
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Publication number: 20090233336Abstract: A method for producing a chondroitin (CH) with a desired sugar chain length; a method for producing a fraction containing a CH with a substantially single sugar chain length; etc. There is provided a method for producing a CH with desired sugar chain length, comprising alternately performing the steps (a) allowing a receptor substrate having a glucuronic acid residue at its non-reducing end (when this step is performed after the step (b), sugar chain obtained by the step (b)), an N-acetylgalactosamine donor and an N-acetylgalactosamine transferase to coexist in a reaction system and (b) allowing a receptor substrate having an N-acetylgalactosamine residue at its non-reducing end (when this step is performed after the step (a), sugar chain obtained by the step (a)), a glucuronic acid donor and a glucuronic acid transferase to coexist in a reaction system.Type: ApplicationFiled: November 16, 2006Publication date: September 17, 2009Applicant: Seikagaku CorporationInventors: Nobuo Sugiura, Satoshi Shimokata, Koji Kimata
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Publication number: 20090191176Abstract: The invention provides a chondroitin sulfate synthesis promoter useful for the treatment of diseases such as articular disease and discopathy. The chondroitin sulfate synthesis promoter contains, as an active ingredient, chondroitin sulfate glucuronyltransferase protein and/or chondroitin sulfate N-acetylgalactosaminyltransferase-1 protein, or a gene encoding the enzyme protein(s).Type: ApplicationFiled: November 18, 2008Publication date: July 30, 2009Inventors: Kenichiro Sakai, Koji Kimata, Kenichi Shinomiya, Hideto Watanabe
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Publication number: 20090155851Abstract: Provided are a method for producing a fraction containing more than 50% of CH represented by the general formula (1), which comprises at least the step of allowing a glucuronic acid donor, an N-acetylgalactosamine donor, a saccharide receptor, a chondroitin polymerase derived from the Escherichia coli K4 strain, and Mn2+ at a final concentration of 0.02 to 100 mM to coexist, and performing a reaction thereof under conditions of 20 to 40° C. and pH 6 to 8 for 0.5 minutes to 4 hours, and a method for producing a fraction containing more than 50% of CH represented by the general formula (2), which comprises at least the step of performing the reaction under same conditions for 10 hours or longer, which enable industrial scale production of a CH fraction of a controlled even number saccharide and odd number saccharide content ratio by a simple procedure at a low cost.Type: ApplicationFiled: August 23, 2006Publication date: June 18, 2009Applicant: Seikagaku CorporationInventors: Nobuo Sugiura, Koji Kimata
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Patent number: 7476730Abstract: A glycosaminoglycan 6-O-sulfotransferase having an activity of transferring sulfate to a hydroxyl at position 6 of a glycosamine residue of a glycosaminoglycan, which has a ratio of relative activities to substrates satisfying completely desulfated N-acetylated (CDSNAc) heparin/completely desulfated N-resulfated (CDSNS) heparin ?0.05 and a molecular weight as calculated from constituent amino acids of from 53,000 to 58,000 daltons.Type: GrantFiled: December 11, 2006Date of Patent: January 13, 2009Assignee: Seikagaku CorporationInventors: Hiroko Habuchi, Koji Kimata
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Patent number: 7470529Abstract: A human-derived novel chondroitin synthase, which is an enzyme for synthesizing a fundamental backbone of chondroitin and has both glucuronic acid transferring activity and N-acetylgalactosamine transferring activity.Type: GrantFiled: May 14, 2007Date of Patent: December 30, 2008Assignees: Seikagaku Kogyo Kabushiki Kaisha, National Institute of Advanced Industrial Science and TechnologyInventors: Hisashi Narimatsu, Koji Kimata, Toshikazu Yada, Takashi Sato, Masanori Goto
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Patent number: 7396818Abstract: The hexuronic acid derivative represented by the following formula 1 or a salt thereof is used as an active ingredient of a heparin/heparan sulfate sulfotransferase inhibitor. In the formula, each of R1, R2, and R3 independently represent(s) SO3? or H which may have a substituent, provided that at least one thereof represents SO3?; X represents OR4, SR4, N(R4)2, or C(R4)3, R4 independently represents H, alkyl, alkenyl, alkynyl, acyl, aryl, or aralkyl group; one of R5 and R6 represents COOH while the other represents H; and the wavy line represents ?-glycosidic bond or ?-glycosidic bond.Type: GrantFiled: May 19, 2004Date of Patent: July 8, 2008Assignee: Seikagaku CorporationInventors: Koji Kimata, Hiroko Habuchi
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Publication number: 20080070996Abstract: A human-derived novel chondroitin synthase, which is an enzyme for synthesizing a fundamental backbone of chondroitin and has both glucuronic acid transferring activity and N-acetylgalactosamine transferring activity.Type: ApplicationFiled: May 14, 2007Publication date: March 20, 2008Inventors: Hisashi Narimatsu, Koji Kimata, Toshikazu Yada, Takashi Sato, Masanori Goto
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Patent number: 7273729Abstract: A human-derived novel chondroitin synthase, which is an enzyme for synthesizing a fundamental backbone of chondroitin and has both glucuronic acid transferase activity and N-acetylgalactosamine transferase activity.Type: GrantFiled: May 30, 2003Date of Patent: September 25, 2007Assignees: Seikagaku Kogyo Kabushiki Kaisha, National Institute of Advanced Industrial Science and TechnologyInventors: Hisashi Narimatsu, Koji Kimata, Toshikazu Yada, Takashi Sato, Masanori Goto