Abstract: An object of the present invention is to provide a biosensor comprising a hydrogel capable of immobilizing a physiologically active substance thereon, which can be produced conveniently by use of a safe material, and a method for producing the same. The present invention provides a method for producing a biosensor, which comprises bringing a polymer containing an activated carboxyl group into contact with a substrate surface coated with an organic layer having an amino group to thereby bind the polymer to the organic layer.

Abstract: An object of the present invention is to provide a biosensor that is capable of immobilizing large quantities of physiologically active substances thereon. The present invention provides a biosensor comprising a substrate having on its surface a polysaccharide capable of chemically immobilizing physiologically active substances, wherein the polysaccharide is a linear polysaccharide or a polysaccharide having 0.3 to 2 primary alcohol groups per unit.

Abstract: It is an object of the present invention to provide a method for carrying out on a same biosensor both of the measurement of the amount of a physiologically active substance immobilized on a biosensor substrate and the measurement of the biological activity of the above physiologically active substance, thereby analyzing the interaction of a substance with the above physiologically active substance that maintains its activity. The present invention provides a measurement method using a biosensor substrate, wherein both the amount of a physiologically active substance immobilized on the substrate and the biological activity of the physiologically active substance immobilized on the substrate are measured on said same biosensor substrate.

Abstract: A flow cell device is incorporated in the sensor unit for surface plasmon resonance (SPR) assay. A box shaped flow cell body has a lower surface, and is disposed in contact of the lower surface with a sensing surface for detecting reaction of sample. Two flow channels are formed in the flow cell body, for flow of the sample. Each of the flow channels include a fluid passageway, formed in the lower surface, for flow of the sample in contact with the sensing surface. Two end cavities are formed in an upper surface of the flow cell body and open therein, to extend from ends of the fluid passageway. The flow channels are arranged adjacent to one another, disposed to extend in a direction of the flow. Fluid passageways of the flow channels are overlapped partially on one another in a flow cell longitudinal direction of the flow cell body.

Abstract: An object of the invention is to reduce the negative drift of the baseline signal when measuring a specific binding reaction between a physiologically active substance and a test substance by using a surface plasmon resonance measurement device. The present invention provides a biosensor comprising a substrate on the surface of which a physiologically active substance is immobilized via noncovalent bond, which is used for a method for detecting or measuring a substance that interacts with the physiologically active substance, with the use of a fluid channel system comprising a cell formed on the substrate, in a state where the flow of the liquid has been stopped after the liquid contained in said flow channel system has been exchanged.

Abstract: A sensor unit of a surface plasmon resonance (SPR) assay system includes a transparent dielectric medium. A thin film has a first surface and a sensing surface. The first surface is connected with the dielectric medium to constitute an interface. The sensing surface is back to the first surface, for detecting (bio)chemical reaction. A flow cell block has a flow channel for flowing of the sample to the sensing surface. Attenuated total reflection of illuminating light is checked at the interface, to analyze interaction between ligand and analyte as samples. The flow channel includes a first inner surface, disposed opposite to the sensing surface to extend along, for passing the sample to flow between. The first inner surface has a height, defined with reference to the sensing surface, and in a range of 200-500 microns.

Abstract: An object of the present invention is to suppress the noise width of a reference cell during measurement and the base line fluctuation. The present invention provides a method for measuring a change in surface plasmon resonance, which comprises: using a surface plasmon resonance measurement device comprising a flow channel system having a cell formed on a metal film and a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of a light beam totally reflected on the meal film; and exchanging the liquid contained in the above flow channel system, wherein the above method is characterized in that a change in surface plasmon resonance is measured in a state where the flow of the liquid has been stopped, after the liquid contained in the above flow channel system has been exchanged.

Abstract: The present invention provides a unit for biochemical analysis wherein the unit comprises a substrate formed of a material having properties of attenuating radiation and/or light and formed with a plurality of holes, and adsorptive areas are respectively formed inside the plurality of holes, thereby forming a plurality of adsorptive areas, and wherein covalently binding functional groups are introduced onto the adsorptive areas. The present invention enables to provide a unit for biochemical analysis which is capable of carrying out strong and efficient immobilization of specific binding substances and can obtain specific and high signals by controlling the direction of the immobilized specific binding substances.

Abstract: A biochemical analysis unit comprises a base plate, which has a plurality of holes, and a porous adsorptive material, which is filled in each of the plurality of the holes of the base plate and forms each of a plurality of adsorptive regions. Each of the adsorptive regions is provided with a signal absorbing layer for absorbing a noise signal, which will otherwise be detected from the adsorptive region. In cases where the porous adsorptive material is connected at one of surfaces of the base plate, the biochemical analysis unit comprises a signal absorbing layer for absorbing a signal, which passes through the porous adsorptive material that is connected at the one surface of the base plate, and which thus propagates from a certain hole of the base plate toward an adjacent hole of the base plate.