Abstract: An ink composition includes core shell titanium oxide particles that each have a core particle and a shell layer covering a surface of the core particle and formed of titanium oxide, and have an average particle diameter of 50 nm or more and 5000 nm or less, silicon oxide particles that have an average particle diameter of 3 nm or more and 100 nm or less, and a resin.

Abstract: A nucleic acid amplification reaction method includes performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a primer and a probe, wherein in the thermal cycling, the time per cycle of the thermal cycling is 9 seconds or less, and the Tm value of the primer is 70° C. or higher and 80° C. or lower.

Abstract: A nucleic acid amplification reaction method includes a heating step of heating a reaction solution containing a reverse transcriptase, a polymerase, a primer, and a probe for performing a reverse transcription reaction, and a thermal cycling step of performing thermal cycling for amplifying a nucleic acid for the reaction solution after the heating step, wherein the Tm value of the primer is 65° C. or higher and 80° C. or lower, and in the heating step, a heating time for the reaction solution is 5 seconds or more and 480 seconds or less.

Abstract: A nucleic acid amplification reaction method includes performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a template nucleic acid, a primer, a probe, and a polymerase, wherein in the thermal cycling, the time per cycle of the thermal cycling is 9 seconds or less, the calculated Tm value of the primer is 65° C. or higher and 80° C. or lower, a ?Tm value obtained by subtracting the actually measured Tm value of the primer from the actually measured Tm value of the probe is ?11° C. or more and 2° C. or less, the calculated Tm value is a value calculated according to a calculation formula, and the actually measured Tm value is an actually measured value obtained by actual measurement.

Abstract: A nucleic acid amplification reaction method includes a thermal cycling step of performing thermal cycling for amplifying a nucleic acid for a reaction solution containing a primer, a temperature increasing step of increasing the temperature of the reaction solution to a temperature at which the amplified nucleic acid is denatured after the thermal cycling step, a temperature decreasing step of decreasing the temperature of the reaction solution to a temperature at which a probe hybridizes after the temperature increasing step, and a probe addition step of adding the probe to the reaction solution.

Abstract: A nucleic acid amplification reagent includes a first probe which anneals to a target nucleic acid in a region sandwiched between a site to which the 3? end of a forward primer anneals and a site to which the 3? end of a reverse primer anneals in a template nucleic acid, and a second probe which anneals to a nucleic acid other than the target nucleic acid in the region. Each of the first probe and the second probe includes a dye which emits light in a wavelength band mutually overlapping partially.

Abstract: A lyophilized reagent according to the invention is a lyophilized reagent for a nucleic acid amplification reaction obtained by lyophilizing a reaction mixture containing a reagent to be used for a nucleic acid amplification reaction and a sugar, wherein the sugar is contained in an amount of 72 mass % or more and 86 mass % or less with respect to the concentration of the solids contained in the lyophilized reagent.

Abstract: A lyophilized material production method according to the invention is a lyophilized material production method for producing a lyophilized material by lyophilizing a reagent containing an additive at a lyophilization temperature, and includes a freezing step in which the reagent containing an additive is cooled with a low-temperature liquid whose temperature is lower than the lyophilization temperature, a maintaining step in which the temperature of the frozen reagent is increased to a temperature higher than the boiling point of the low-temperature liquid and the reagent is maintained at the temperature for a predetermined time, and a lyophilization step in which the reagent having been maintained for a predetermined time after increasing the temperature of the reagent is dried at the lyophilization temperature under reduced pressure.

Abstract: A nucleic acid extraction device includes a tube part in which a first plug of an oil, a second plug of a cleansing liquid not miscible with the oil for cleansing a material with adsorbed nucleic acid, a third plug of an oil, a fourth plug of an eluate not miscible with the oil for eluting nucleic acid from the material, and a fifth plug of an oil are provided in this order, and a cover part provided to surround the tube part.

Abstract: A nucleic acid amplification reaction apparatus includes: a fitting section capable of fitting a reaction vessel filled with a reaction mixture and a liquid which has a specific gravity different from that of the reaction mixture and is immiscible with the reaction mixture; a first heating section which heats a first region of the reaction vessel; a second heating section which heats a second region of the reaction vessel; and a driving mechanism which switches over between a first arrangement in which the first region is located lower than the second region in the direction of the gravitational force and a second arrangement in which the second region is located lower than the first region in the direction of the gravitational force, wherein the concentration of magnesium ions in the reaction mixture is 1.8 mM or more and 9.0 mM or less.

Abstract: A cartridge set includes: a first package; a cleaning container that is accommodated and sealed in the first package while accommodating and sealing a cleaning solution for cleaning a nucleic acid-binding solid-phase carrier to which nucleic acid adheres; an elution container that is accommodated and sealed in the first package while accommodating and sealing an elution solution which elutes the nucleic acid from the nucleic acid-binding solid-phase carrier; a second package; and a reaction container that is accommodated and sealed in the second package while accommodating and sealing a reagent for performing a nucleic acid amplification reaction. The first package has water permeability that is lower than that of the cleaning container and the elution container. The second package has water permeability that is lower than that of the reaction container.

Abstract: A cartridge set includes: a first package; a cleaning container that is accommodated and sealed in the first package while accommodating and sealing a cleaning solution for cleaning a nucleic acid-binding solid-phase carrier to which nucleic acid adheres; a second package; an elution container that is accommodated and sealed in the second package while accommodating and sealing an elution solution which elutes the nucleic acid from the nucleic acid-binding solid-phase carrier; a third package; and a reaction container that is accommodated and sealed in the third package while accommodating and sealing a reagent for performing a nucleic acid amplification reaction, in which the first package has water permeability that is lower than that of the cleaning container, the second package has water permeability that is lower than that of the elution container, and the third package has water permeability that is lower than that of the reaction container.

Abstract: A container storage receptacle includes a package, a cleaning container that is enclosed and stored in the package, and that encloses and stores a cleaning solution for cleaning a nucleic acid binding activity solid-phase carrier which has an adsorbed nucleic acid, and an elution container that is enclosed and stored in the package, and that encloses and stores an eluent for eluting the nucleic acid from the nucleic acid binding activity solid-phase carrier. The cleaning solution and the eluent contain water, and an inner portion of the package is in a state of being saturated with water vapor.

Abstract: A container storage receptacle includes a package, and a cleaning container that is enclosed and stored in the package, and that encloses and stores a cleaning solution for cleaning a nucleic acid binding activity solid-phase carrier which has an adsorbed nucleic acid. The cleaning solution contains alcohol, and an inner portion of the package is in a state of being saturated with alcohol vapor.

Abstract: A container storage receptacle includes a package and an elution container that is enclosed and stored in the package, and that encloses and stores an eluent for eluting a nucleic acid from a nucleic acid binding activity solid-phase carrier. The eluent contains water, and an inner portion of the package is in a state of being saturated with water vapor.

Abstract: A container accommodation body includes: a package; a container that is accommodated and sealed in the package while accommodating and sealing a reagent for performing a nucleic acid amplification reaction; and a drying agent that is accommodated and sealed in the package.

Abstract: Method for concentrating and collecting small quantities of fetal nucleated red blood cells contained in the maternal blood. The method for concentrating and collecting nucleated red blood cells from the maternal blood comprises: (i) subjecting the maternal blood to a first density-gradient centrifugation and collecting a cell fraction containing nucleated red blood cells; (ii) treating the cell fraction containing nucleated red blood cells so as to selectively changes the density of the nucleated red blood cells from that of the white blood cells; and (iii) subjecting the treated cell fraction containing the nucleated red blood cells to a second density-gradient centrifugation so as to collect a fraction containing nucleated red blood cells.

Abstract: A method for discriminating the presence or absence of a mutation in a DNA having a plurality of mutated forms includes performing a thermal cycle for amplifying the DNA in the presence of a pair of primers which can bind to the DNA, a first probe which does not bind to the DNA having a mutation, can bind to the DNA having no mutation, and is labeled with a first fluorescent substance, and a second probe which can bind to both of the DNA having a mutation and the DNA having no mutation and is labeled with a second fluorescent substance which emits a light having a fluorescence wavelength different from that of a light emitted from the first fluorescent substance.

Abstract: A lyophilizate containing a substance-binding solid-phase carrier, which is obtained by lyophilizing the substance-binding solid-phase carrier together with an additive, a vessel for binding a substance in a substance-containing liquid to a substance-binding solid-phase carrier, in which a lyophilizate obtained by lyophilizing the substance-binding solid-phase carrier together with an additive is provided, and a method of producing a lyophilizate containing a substance-binding solid-phase carrier, which includes lyophilizing the substance-binding solid-phase carrier together with an additive, are provided.

Abstract: A cartridge for nucleic acid amplification reaction includes a tube that is internally provided with, in the following order, in a longitudinal direction, a first plug composed of oil, a second plug composed of a first washing liquid, a third plug composed of oil, a fourth plug composed of an eluent, and a fifth plug composed of oil, and a container for nucleic acid amplification reaction that is disposed to communicate with an end portion of the tube on a side on which the fifth plug is disposed and contains oil, in which a freeze-dried nucleic acid amplification reaction reagent does not dissolve in the oil of the fifth plug and is held in the oil.