Abstract: As a method for producing a 3-hydroxyisobutyric acid ester using a biocatalyst, a method for producing a 3-hydroxyisobutyric acid ester, including a step of allowing an alcohol or phenol to act on 3-hydroxyisobutyryl-CoA in the presence of alcohol acyltransferase to produce a 3-hydroxyisobutyric acid ester is provided.

Abstract: Provided is a method that is for producing acrylamide, continuously produces acrylamide from acrylonitrile using a biocatalyst, and is characterized in that a continuous reaction is started by means of introducing acrylamide into a reaction vessel and then causing acrylonitrile to contact a biocatalyst. The method for producing acrylamide has superior operability and a low cost, and is capable of continuously extracting an aqueous solution of acrylamide at a target concentration in a short period of time after starting the continuous reaction.

Abstract: The invention pertains to a technique for suppressing a decrease in nitrile hydratase activity and improving the productivity of the amide compound in the course of producing an amide compound from a nitrile compound using a biocatalyst. Specifically, the invention pertains to a method for producing the corresponding amide compound from a nitrile compound in the presence of a biocatalyst having nitrile hydratase activity, wherein the method for producing an amide compound using a nitrile compound is characterized in that the zinc concentration of the nitrile compound is 0.4 ppm or less.

Abstract: Provided is a method that is for producing acrylamide, continuously produces acrylamide from acrylonitrile using a biocatalyst, and is characterized in that a continuous reaction is started by means of introducing acrylamide into a reaction vessel and then causing acrylonitrile to contact a biocatalyst. The method for producing acrylamide has superior operability and a low cost, and is capable of continuously extracting an aqueous solution of acrylamide at a target concentration in a short period of time after starting the continuous reaction.

Abstract: The invention pertains to a technique for suppressing a decrease in nitrile hydratase activity and improving the productivity of the amide compound in the course of producing an amide compound from a nitrile compound using a biocatalyst. Specifically, the invention pertains to a method for producing the corresponding amide compound from a nitrile compound in the presence of a biocatalyst having nitrile hydratase activity, wherein the method for producing an amide compound using a nitrile compound is characterized in that the zinc concentration of the nitrile compound is 0.4 ppm or less.

Abstract: The present invention provides a more efficient method for producing acrylamide from acrylonitrile by the action of a microbially-derived enzyme, nitrile hydratase. More specifically, the present invention provides a method for producing acrylamide from acrylonitrile using a biocatalyst having nitrile hydratase, which comprises the step of keeping acrylonitrile while cooling to less than 30° C. Moreover, the present invention also provides an apparatus for producing acrylamide from acrylonitrile using a biocatalyst having nitrile hydratase.

Abstract: [Problem] The present invention provides a method for producing acrylamide from acrylonitrile using a biocatalyst with high productivity at low cost, in which reaction heat can be efficiently removed even at an industrial reaction scale. [Means for Solving] The present invention relates to a method for producing acrylamide by reacting acrylonitrile in the presence of a biocatalyst, wherein the reaction is performed using a reactor having a reaction volume of 1 m3 or more equipped with a tubular heat exchanger, and wherein the difference between a temperature of a cooling water to be introduced into the heat exchanger and a reaction temperature is maintained from 5 to 20° C.

Abstract: In a method for producing an amide compound of the present invention including obtaining an amide compound from a nitrile compound using a microbial catalyst and transferring a solution containing the microbial catalyst and the amide compound, a positive-displacement pump is used for transferring the solution containing a microbial catalyst and an amide compound to obtain an amide compound having few impurities. A monomer including (meth)acrylamide obtained by the method for producing an amide compound is polymerized to prepare an acrylamide polymer having a high-molecular mass and high solubility and being colorless.

Abstract: This invention relates to a process for producing an amide compound from a nitrile compound using a microbial catalyst, wherein a microbial cell having nitrile hydratase activity of 50 U or higher per mg of dry cell at a reaction temperature of 10° C. is brought into contact with a nitrile compound in an aqueous medium without being immobilized. This method utilizes a microbial cell that exhibits high nitrile hydratase activity in the reaction without being entrap-immobilized. Thus, an amide compound can be effectively produced from a nitrile compound without problems of decreased reaction speed or lowered amount produced per unit cell amount, which are caused by entrap-immobilization. Accordingly, an amide compound can be produced within a very short period of time in the case of a batch reaction and with a very small-scale facility in the case of a continuous reaction.

Abstract: In a method for producing an amide compound of the present invention including obtaining an amide compound from a nitrile compound using a microbial catalyst and transferring a solution containing the microbial catalyst and the amide compound, a positive-displacement pump is used for transferring the solution containing a microbial catalyst and an amide compound to obtain an amide compound having few impurities. A monomer including (meth)acrylamide obtained by the method for producing an amide compound is polymerized to prepare an acrylamide polymer having a high-molecular mass and high solubility and being colorless.

Abstract: The object of the present invention is to obtain a highly soluble and colorless acrylamide polymer having a high molecular weight. A method for producing such acrylamide polymer involves comprises steps of: hydrating acrylonitrile containing oxazole at a concentration of 5 mg/kg or less and hydrogen cyanide at a concentration of 1 mg/kg or less by an enzymatic method to yield acrylamide; andt polymerizing monomers containing acrylamide.

Abstract: This invention provides an aqueous solution of acrylamide that is useful as a starting material for high-quality polyacrylamide. Starting materials for polyacrylamide are an aqueous solution of acrylamide containing a saccharide and an aqueous solution of acrylamide containing a saccharide produced with the use of a biocatalyst having nitrile hydratase activity.

Abstract: Provided is a method for producing a compound using a biocatalyst. In the method for continuously producing a compound using a biocatalyst in one or a plurality of reaction tanks, the downstream reaction temperature is set higher than the upstream reaction temperature of the flow of the catalyst in a reaction tank or between reaction tanks.

Abstract: This invention provides an aqueous solution of acrylamide that is useful as a starting material for high-quality polyacrylamide. Starting materials for polyacrylamide are an aqueous solution of acrylamide containing a saccharide and an aqueous solution of acrylamide containing a saccharide produced with the use of a biocatalyst having nitrile hydratase activity.

Abstract: Provided is a method for producing a compound using a biocatalyst. In the method for continuously producing a compound using a biocatalyst in one or a plurality of reaction tanks, the downstream reaction temperature is set higher than the upstream reaction temperature of the flow of the catalyst in a reaction tank or between reaction tanks.

Abstract: This invention relates to a process for producing an amide compound from a nitrile compound using a microbial catalyst, wherein a microbial cell having nitrile hydratase activity of 50 U or higher per mg of dry cell at a reaction temperature of 10° C. is brought into contact with a nitrile compound in an aqueous medium without being immobilized. This method utilizes a microbial cell that exhibits high nitrile hydratase activity in the reaction without being entrap-immobilized. Thus, an amide compound can be effectively produced from a nitrile compound without problems of decreased reaction speed or lowered amount produced per unit cell amount, which are caused by entrap-immobilization. Accordingly, an amide compound can be produced within a very short period of time in the case of a batch reaction and with a very small-scale facility in the case of a continuous reaction.