Abstract: The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilized pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel.

Abstract: The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilised pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel.

Abstract: The present invention relates to methods and systems for adding a reagent to an analyte in a gel. The invention further provides methods and systems for transferring liquid analyte reagent mixtures from a gel to a second vessel, such as a microtitre plate. The invention is useful in the manipulation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for manipulating proteins and peptides in isoelectric focusing gels.

Abstract: The present invention relates to an electrode bridge or sample application bridge for use in electrophoresis, preferably isoelectric focusing, IEF. More closely, the invention relates to an electrodic bridge or sample loading bridge for preventing denaturant depletion from the IPG (immobilized pH gradient) gel and, optionally, for loading samples onto the IPG gel. The pH of the bridge is adjustable for adoption to positioning at the acid as well as basic end of the IPG strips.

Abstract: The present invention relates to a method, composition and a kit for isoelectric focusing. More closely, the present invention involves the use of to modified carrier ampholytes which are easy to separate from the proteins/peptides following finished focusing. The modification is that the carrier ampholytes are derivatised with handles interacting with a solid phase/matrix which makes them easy to remove and separate from the peptides after finished focusing. In this way, there is very little or no background from carrier ampholytes in the following MS-spectra.

Abstract: The present invention relates to an electrode bridge or sample application bridge for use in electrophoresis, preferably isoelectric focusing, IEF. More closely, the invention relates to an electrodic bridge or sample loading bridge for preventing denaturant depletion from the IPG (immobilised pH gradient) gel and, optionally, for loading samples onto the IPG gel. The pH of the bridge is adjustable for adoption to positioning at the acid as well as basic end of the IPG strips.

Abstract: The present invention relates to methods and systems for adding a reagent to an analyte in a gel. The invention further provides methods and systems for transferring liquid analyte reagent mixtures from a gel to a second vessel, such as a microtitre plate. The invention is useful in the manipulation of biological molecules such as nucleic acids, carbohydrates, proteins and peptides. In particular, the invention has utility for manipulating proteins and peptides in isoelectric focusing gels.

Abstract: The present invention relates to a method, composition and a kit for isoelectric focusing. More closely, the present invention involves the use of to modified carrier ampholytes which are easy to separate from the proteins/peptides following finished focusing. The modification is that the carrier ampholytes are derivatised with handles interacting with a solid phase/matrix which makes them easy to remove and separate from the peptides after finished focusing. In this way, there is very little or no background from carrier ampholytes in the following MS-spectra.

Abstract: The present invention relates to the field of electrophoresis and more specifically to a gel or strip for separating peptide components by isoelectric focusing by producing IPG (immobilised pH gradient) gels or strips in a novel way before focusing. More closely, the peptides are focused in a novel IPG gel including an uneven or non-linear pH gradient having at least three separate stepwise arranged pH-intervals. After focusing the peptide resolution is high and the peptides are evenly distributed along the gel.