Abstract: There is disclosed a combinatory separation system comprising: a first compartment adapted to handle a mixture with magnetic beads having an affinity for molecules, comprising a magnet to attract the beads, a second compartment adapted to receive said magnetic beads transferred thereto with a flow of liquid, and said at least one second compartment is adapted to elute said at least one type of molecules to be separated from said magnetic beads, said at least one second compartment has an outlet comprising means for retaining said magnetic beads in the at least one second compartment. Advantages include that the process becomes simpler quicker and thereby less expensive, since the method is able to remove cells and particles, separate molecules and perform virus inactivation in fewer steps compared to the prior art. Further the method is able to provide a more concentrated end product.

Abstract: There is provided a process for the separation of molecules from a suspension comprising cells at a concentration of at least 56×106 cells/ml, comprising the steps of: a) providing magnetic particles having a specific interaction with said molecules to be separated, b) mixing the magnetic particles with the cell suspension containing the molecules, c) bringing the cell suspension in contact with a magnetic field provided by a magnetic separation device to collect the magnetic particles, d) decreasing or removing said magnetic field and collecting said magnetic particles carrying said molecules, and e) removing said molecules from said magnetic particles, to provide a concentrated fraction of said molecules, and/or provide partial or complete removal of impurities and cells from the fraction containing the molecules. Advantages include that the yield increases, the volume of the bioreactor and other equipment can be made smaller so that the process becomes more economical.

Abstract: There is disclosed a combinatory separation system comprising: a first compartment adapted to handle a mixture with magnetic beads having an affinity for molecules, comprising a magnet to attract the beads, a second compartment adapted to receive said magnetic beads transferred thereto with a flow of liquid, and said at least one second compartment is adapted to elute said at least one type of molecules to be separated from said magnetic beads, said at least one second compartment has an outlet comprising means for retaining said magnetic beads in the at least one second compartment. Advantages include that the process becomes simpler quicker and thereby less expensive, since the method is able to remove cells and particles, separate molecules and perform virus inactivation in fewer steps compared to the prior art. Further the method is able to provide a more concentrated end product.

Abstract: A method for the production of particles by reacting functional groups on the surface of non-magnetic porous particles with functional groups on the surface of magnetic particles to form a covalent bond, to obtain particles supplemented with magnetic particles covalently bound to the outer part of said particles. Advantages include an increased binding capacity.

Abstract: A novel system for the analysis of molecules and cells, comprising clusters where a non-magnetic particle is supplemented with magnetic particles to form a characteristic pattern, fingerprint or bar code. Methods and devices for formation of such particles are also disclosed.

Abstract: A process for large scale separation of molecules comprising the steps of providing magnetic porous particles having an affinity to said molecules to be separated; mixing said magnetic porous particles with a solution containing said molecules; bringing said mixture in contact with a magnetic separation device comprising a flow channel and at least one magnetic element; removing said at least one magnetic element and collecting the magnetic porous particles carrying said molecules; separating said molecules from said magnetic porous particles; obtaining a concentrated fraction of said molecules; and recirculating the magnetic porous particles. A system for performing this process.

Abstract: A method for the production of particles by reacting functional groups on the surface of non-magnetic porous particles with functional groups on the surface of magnetic particles to form a covalent bond, to obtain particles supplemented with magnetic particles covalently bound to the outer part of said particles. Advantages include an increased binding capacity.

Abstract: A novel system for the analysis of molecules and cells, comprising clusters where a non-magnetic particle is supplemented with magnetic particles to form a characteristic pattern, fingerprint or bar code. Methods and devices for formation of such particles are also disclosed.

Abstract: There is provided a method for covalent immobilization of at least one molecule comprising at least one amino group, said method comprising the sequential steps of: a) providing a surface comprising —SH groups, b) oxidizing the surface comprising —SH groups using redox reactions in the presence of noble metal ions, and c) contacting the surface with at least one molecule comprising at least one amino group to obtain a covalent binding of the at least one molecule to the surface, wherein said at least one amino group is involved in obtaining said covalent bond. The immobilized molecules are immobilized via stable covalent bonds. The method is more versatile since it can be performed as a one step method. All reaction steps are performed in aqueous solution. All steps can be performed at room temperature. The chemicals used are less expensive and less toxic compared to the prior art.

Abstract: There is provided a method for covalent immobilization of at least one molecule comprising at least one amino group, said method comprising the sequential steps of: a) providing a surface comprising —SH groups, b) oxidizing the surface comprising —SH groups using redox reactions in the presence of noble metal ions, and c) contacting the surface with at least one molecule comprising at least one amino group to obtain a covalent binding of the at least one molecule to the surface, wherein said at least one amino group is involved in obtaining said covalent bond. The immobilized molecules are immobilized via stable covalent bonds. The method is more versatile since it can be performed as a one step method. All reaction steps are performed in aqueous solution. All steps can be performed at room temperature. The chemicals used are less expensive and less toxic compared to the prior art.