Patents by Inventor Kristopher A Kilian
Kristopher A Kilian has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220119773Abstract: The invention relates to a method for improving angiogenic potential of a mesenchymal stem cell (MSC), the method comprising culturing the MSC on a substrate having stiffness of about 1 kPa to 100 kPa and coated with a matrix protein, wherein the MSC has improved angiogenic potential when compared with a MSC cultured under identical conditions except not cultured on a substrate having stiffness of about 1 kPa to 100 kPa and not coated with a matrix protein. The invention also relates to a MSC having angiogenic potential when improved by the method, and to therapeutic use of the improved MSC for treating coronary artery disease (CAD) or peripheral artery disease (PAD) in a subject having CAD or PAD.Type: ApplicationFiled: February 21, 2020Publication date: April 21, 2022Applicant: CYNATA THERAPEUTICS LIMITEDInventors: Kristopher KILIAN, Sara ROMANAZZO
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Patent number: 9347940Abstract: A method of component assembly on a substrate, and an assembly of a bound component on a substrate. The method comprises the steps of forming a free-standing component having an optical characteristic; providing a pattern of a first binding species on the substrate or the free standing component; and forming a bound component on the substrate through a binding interaction via the first binding species; wherein the bound component exhibits substantially the same optical characteristic compared to the free-standing component.Type: GrantFiled: April 11, 2014Date of Patent: May 24, 2016Assignee: TAMIRAS PER PTE. LTD., LLCInventors: Till Bocking, John Justin Gooding, Kristopher A. Kilian, Michael Gal, Katharina Gaus, Peter John Reece, Qiao Hong
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Publication number: 20150118729Abstract: Disclosed herein are compositions and methods for programming a cell. The compositions include a substrate and a cell adhesion agent. The substrate includes a surface having a micropatterned object and the cell adhesion agent is immobilized within a first area defined by the micropatterned object.Type: ApplicationFiled: October 29, 2014Publication date: April 30, 2015Inventors: Kristopher A. Kilian, Junmin Lee, Yanfen Li
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Patent number: 8979770Abstract: The present invention provides devices, systems and methods for RNA isolation from biological samples containing white blood cells, such as whole blood. The devices have a device body that includes a first chamber having a first membrane that selectively binds white blood cells, a second chamber having a second membrane that reversibly binds RNA and a plurality of ports that are removably attached to a reagent pack and waste receptacle via a valving system controlled by a control unit.Type: GrantFiled: February 15, 2007Date of Patent: March 17, 2015Assignee: Merck Sharp & Dohme Corp.Inventors: Thomas L. Fare, Deborah A. Kessler, Kristopher A. Kilian
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Publication number: 20140220670Abstract: A method of component assembly on a substrate, and an assembly of a bound component on a substrate. The method comprises the steps of forming a free-standing component having an optical characteristic; providing a pattern of a first binding species on the substrate or the free standing component; and forming a bound component on the substrate through a binding interaction via the first binding species; wherein the bound component exhibits substantially the same optical characteristic compared to the free-standing component.Type: ApplicationFiled: April 11, 2014Publication date: August 7, 2014Applicant: Newsouth Innovations Pty LimitedInventors: Till Bocking, John Justin Gooding, Kristopher A. Kilian, Michael Gal, Katharina Gaus, Peter John Reece, Qiao Hong
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Patent number: 8722437Abstract: A method of component assembly on a substrate, and an assembly of a bound component on a substrate. The method comprises the steps of forming a free-standing component having an optical characteristic; providing a pattern of a first binding species on the substrate or the free standing component; and forming a bound component on the substrate through a binding interaction via the first binding species; wherein the bound component exhibits substantially the same optical characteristic compared to the free-standing component.Type: GrantFiled: October 31, 2008Date of Patent: May 13, 2014Assignee: Mogul Solutions LLCInventors: Till Böcking, John Justin Gooding, Kristopher A. Kilian, Michael Gal, Katharina Gaus, Peter John Reece, Qiao Hong
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Publication number: 20120149602Abstract: The present invention provides methods of making a population of nucleic acid molecules, wherein each nucleic acid molecule comprises a predetermined nucleic acid sequence, each of said methods comprising the steps of: (a) synthesizing, on a substrate, a population of nucleic acid molecules wherein: i) each synthesized nucleic acid molecule comprises a predetermined nucleic acid sequence; and ii) each synthesized nucleic acid molecule is localized to a defined area of said substrate; (b) harvesting said population of synthesized nucleic acid molecules from said substrate to yield harvested nucleic acid molecules; and (c) introducing said harvested nucleic acid molecules into vector molecules.Type: ApplicationFiled: February 16, 2012Publication date: June 14, 2012Inventors: Stephen H. FRIEND, Michele A. CLEARY, Kristopher A. KILIAN, Ernest M. COFFEY, Gregory J. HANNON, Patrick PADDISON
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Patent number: 8133670Abstract: The present invention provides methods of making a population of nucleic acid molecules, wherein each nucleic acid molecule comprises a predetermined nucleic acid sequence, each of said methods comprising the steps of: (a) synthesizing, on a substrate, a population of nucleic acid molecules wherein: i) each synthesized nucleic acid molecule comprises a predetermined nucleic acid sequence; and ii) each synthesized nucleic acid molecule is localized to a defined area of said substrate; (b) harvesting said population of synthesized nucleic acid molecules from said substrate to yield harvested nucleic acid molecules; and (c) introducing said harvested nucleic acid molecules into vector molecules.Type: GrantFiled: June 2, 2004Date of Patent: March 13, 2012Assignees: Cold Spring Harbor Laboratory, Merck & Co., Inc.Inventors: Stephen H Friend, Michele A Cleary, Kristopher A Kilian, Ernest M Coffey, Gregory J Hannon, Patrick Paddison
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Publication number: 20100279394Abstract: A method of component assembly on a substrate, and an assembly of a bound component on a substrate. The method comprises the steps of forming a free-standing component having an optical characteristic; providing a pattern of a first binding species on the substrate or the free standing component; and forming a bound component on the substrate through a binding interaction via the first binding species; wherein the bound component exhibits substantially the same optical characteristic compared to the free-standing component.Type: ApplicationFiled: October 31, 2008Publication date: November 4, 2010Applicant: NEWSOUTH INNOVATIONS PTY LIMITEDInventors: Till Böcking, John Justin Gooding, Kristopher A. Kilian, Michael Gal, Katharina Gaus, Peter John Reece, Qiao Hong
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Publication number: 20100113287Abstract: The invention provides methods for evaluating the representation of expected nucleic acid molecules in a test population of nucleic acid molecules. The methods each comprise the steps of: (a) hybridizing a population of sample nucleic acid molecules obtained from a test population of nucleic acid molecules to a substrate comprising a population of target nucleic acid molecules, wherein (i) each target nucleic acid molecule comprises a predetermined sequence corresponding to an expected nucleic acid molecule, and (ii) each target nucleic acid molecule is localized to a defined area of the substrate; and (b) evaluating the representation of expected nucleic acid molecules in the test population of nucleic acid molecules by analyzing the pattern of hybridization of the sample population of nucleic acid molecules to the target nucleic acid molecules.Type: ApplicationFiled: September 21, 2009Publication date: May 6, 2010Applicant: Rosetta Inpharmatics LLCInventors: Michele A. Cleary, Kristopher A. Kilian, Hongyue Dai, Yanqun Wang
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Publication number: 20090117339Abstract: A method of component assembly on a substrate, and an assembly of a bound component on a substrate. The method comprises the steps of forming a free-standing component having an optical characteristic; providing a pattern of a first binding species on the substrate or the free standing component; and forming a bound component on the substrate through a binding interaction via the first binding species; wherein the bound component exhibits substantially the same optical characteristic compared to the free-standing component.Type: ApplicationFiled: November 1, 2007Publication date: May 7, 2009Inventors: Till Bocking, John Justin Gooding, Kristopher A. Kilian, Michael Gal, Katharina Gaus
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Publication number: 20040259146Abstract: The present invention provides methods of making a population of nucleic acid molecules, wherein each nucleic acid molecule comprises a predetermined nucleic acid sequence, each of said methods comprising the steps of: (a) synthesizing, on a substrate, a population of nucleic acid molecules wherein: i) each synthesized nucleic acid molecule comprises a predetermined nucleic acid sequence; and ii) each synthesized nucleic acid molecule is localized to a defined area of said substrate; (b) harvesting said population of synthesized nucleic acid molecules from said substrate to yield harvested nucleic acid molecules; and (c) introducing said harvested nucleic acid molecules into vector molecules.Type: ApplicationFiled: June 2, 2004Publication date: December 23, 2004Applicants: Rosetta Inpharmatics LLC, Cold Spring Harbor LaboratoryInventors: Stephen H. Friend, Michele A. Cleary, Kristopher A. Kilian, Ernest M. Coffey, Gregory J. Hannon