Patents by Inventor Kuniki Kino
Kuniki Kino has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220136015Abstract: A method for producing an oxygen adduct of sesquiterpene, comprising: a step of providing a system comprising an iron reduction unit; a step of adding a trivalent iron compound to the system; and an oxygen addition step of performing oxygen addition to sesquiterpene in the system comprising the iron reduction unit and the trivalent iron compound is a novel method that can efficiently produce an oxygen adduct of sesquiterpene.Type: ApplicationFiled: February 21, 2020Publication date: May 5, 2022Inventors: Satoru UMEZAWA, Shunsuke KONISHI, Kuniki KINO
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Publication number: 20200263222Abstract: A method for producing imidazole dipeptide using a microorganism having imidazole dipeptide synthesis activity, the production method not including adding ATP, or including adding an amount of ATP that is less than the amount of imidazole dipeptide that is produced in terms of the number of moles, by utilizing an ATP supply system of the microorganism.Type: ApplicationFiled: February 12, 2020Publication date: August 20, 2020Inventor: Kuniki KINO
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Patent number: 10704036Abstract: The present invention provides L-amino acid ?-ligase represented by sequence number 1 (SEQ ID NO. 1), which is a mutant protein including substitution of at least 1-3 amino acid residues of amino acid sequence of protein YwfE, with the substitution being at least one of an asparagine (N) residue of the 108th place from the N terminal being substituted with at least one of an alanine (A) residue, a glutamic acid (E) residue and a glutamine (Q) residue, an isoleucine (I) residue of the 112th place from the N terminal being substituted with a valine (V) residue, and a histidine (H) residue of the 378th place from the N terminal being substituted with at least one of a lysine (K) residue or an arginine (R) residue.Type: GrantFiled: December 27, 2017Date of Patent: July 7, 2020Assignee: TOKAI BUSSAN CO., LTD.Inventor: Kuniki Kino
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Patent number: 10392673Abstract: The present invention provides a method of producing (?)-rotundone from ?-guaiene. The method includes the steps of: (1) allowing a cytochrome P450 protein to act on ?-guaiene, which cytochrome P450 belongs to the CYP152 family and is capable of oxidizing the methylene group at position 3 of ?-guaiene to the carbonyl group; and/or (2) allowing a cytochrome P450 protein to act on ?-guaiene in the presence of an electron transfer protein capable of transferring electrons to the cytochrome P450 protein, which cytochrome P450 belongs to the CYP152, CYP106, or CYP107 family and is capable of oxidizing the methylene group at position 3 of ?-guaiene to the carbonyl group.Type: GrantFiled: May 31, 2017Date of Patent: August 27, 2019Assignee: T. HASEGAWA CO., LTD.Inventors: Kuniki Kino, Toshiki Furuya
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Publication number: 20180179511Abstract: The present invention provides L-amino acid ?-ligase represented by sequence number 1 (SEQ ID NO. 1), which is a mutant protein including substitution of at least 1-3 amino acid residues of amino acid sequence of protein YwfE, with the substitution being at least one of an asparagine (N) residue of the 108th place from the N terminal being substituted with at least one of an alanine (A) residue, a glutamic acid (E) residue and a glutamine (Q) residue, an isoleucine (I) residue of the 112th place from the N terminal being substituted with a valine (V) residue, and a histidine (H) residue of the 378th place from the N terminal being substituted with at least one of a lysine (K) residue or an arginine (R) residue.Type: ApplicationFiled: December 27, 2017Publication date: June 28, 2018Inventor: Kuniki Kino
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Publication number: 20170356059Abstract: The present invention provides a method of producing (?)-rotundone from ?-guaiene. The method includes the steps of: (1) allowing a cytochrome P450 protein to act on ?-guaiene, which cytochrome P450 belongs to the CYP152 family and is capable of oxidizing the methylene group at position 3 of ?-guaiene to the carbonyl group; and/or (2) allowing a cytochrome P450 protein to act on ?-guaiene in the presence of an electron transfer protein capable of transferring electrons to the cytochrome P450 protein, which cytochrome P450 belongs to the CYP152, CYP106, or CYP107 family and is capable of oxidizing the methylene group at position 3 of ?-guaiene to the carbonyl group.Type: ApplicationFiled: May 31, 2017Publication date: December 14, 2017Applicant: T. HASEGAWA CO., LTD.Inventors: Kuniki KINO, Toshiki FURUYA
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Publication number: 20170037437Abstract: The present invention is directed to provide novel methods of producing hydroxystilbene. According to the present invention, as a method of producing a compound having the general formula (II), in which R1 to R3 are each —H, —OH or —OR; R is independently selected from a C1-6 alkyl group and a glycosyl group; and one of R4 and R5 is —H and the other is —OH, 4-hydroxyphenylacetate 3-hydroxylase (HPAH) is administered to a compound having the general formula (I), in which R1 to R3 are each —H, —OH or —OR; and R is independently selected from a C1-6 alkyl group and a glycosyl group.Type: ApplicationFiled: January 6, 2015Publication date: February 9, 2017Inventors: Kuniki KINO, Toshiki FURUYA
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Publication number: 20160374380Abstract: Provided is a salty taste enhancer that is capable of maintaining the flavor balance inherent in a food and enhancing salty taste despite a reduction in salt content. The salty taste enhancer effectively enhances salty taste by adding to a food or beverage having salty taste at least one selected from the group consisting of prolylglycine, glycylglycine, glycylproline and hydroxyprolylglycine, or a gelatin degradation product containing the dipeptide.Type: ApplicationFiled: June 20, 2016Publication date: December 29, 2016Applicant: T. HASEGAWA CO., LTD.Inventors: Haruka KINO, Masanao KAKUTANI, Koichi HATTORI, Kuniki KINO
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Patent number: 9512452Abstract: The present invention aims to provide a method for efficiently producing hydroxy-L-lysine. The present invention provides a method for producing hydroxy-L-lysine, the method comprising allowing 2-oxoglutarate-dependent L-lysine hydroxylase, a cell containing 2-oxoglutarate-dependent L-lysine hydroxylase, a processed product of the cell, and/or a culture broth obtained by culturing the cell, to act on L-lysine to produce hydroxy-L-lysine represented by the following General Formula (I) (wherein each of R1, R2 and R3 represents a hydrogen atom or hydroxyl group, with the proviso that at least one of R1, R2 and R3 represents a hydroxyl group).Type: GrantFiled: February 18, 2014Date of Patent: December 6, 2016Assignee: API CorporationInventors: Kuniki Kino, Ryotaro Hara, Ryoma Miyake, Hiroshi Kawabata
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Publication number: 20150259715Abstract: The present invention aims to provide a method for efficiently producing hydroxy-L-lysine. The present invention provides a method for producing hydroxy-L-lysine, the method comprising allowing 2-oxoglutarate-dependent L-lysine hydroxylase, a cell containing 2-oxoglutarate-dependent L-lysine hydroxylase, a processed product of the cell, and/or a culture broth obtained by culturing the cell, to act on L-lysine to produce hydroxy-L-lysine represented by the following General Formula (I) (wherein each of R1, R2 and R3 represents a hydrogen atom or hydroxyl group, with the proviso that at least one of R1, R2 and R3 represents a hydroxyl group).Type: ApplicationFiled: February 18, 2014Publication date: September 17, 2015Applicant: API CorporationInventors: Kuniki Kino, Ryotaro Hara, Ryoma Miyake, Hiroshi Kawabata
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Patent number: 9081199Abstract: The present invention provides: a protein having dipeptide-synthesizing activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant transformed with the recombinant DNA; a process for producing the protein having dipeptide-synthesizing activity using the transformant or the like; a process for producing a dipeptide using the protein having dipeptide-synthesizing activity; and a process for producing a dipeptide using, as an enzyme source, a culture of a transformant or a microorganism which produces the protein having dipeptide-synthesizing activity or the like.Type: GrantFiled: March 17, 2006Date of Patent: July 14, 2015Assignee: KYOWA HAKKO BIO CO., LTD.Inventors: Kuniki Kino, Yuji Nakazawa, Makoto Yagasaki
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Patent number: 8883459Abstract: Development of a method of economically and efficiently producing cis-4-hydroxy-L-proline. The present invention provides L-proline cis-4-hydroxylase. This enzyme may be derived from Lotus corniculatus rhizobia, Mesorhizobium loti or Medicago sativa rhizobia, Sinorhizobium meliloti. The present invention provides a method of producing cis-4-hydroxy-L-proline from L-proline by using this enzyme. The present invention provides a recombinant vector containing a polynucleotide encoding the enzyme and a transformant containing the vector.Type: GrantFiled: August 19, 2013Date of Patent: November 11, 2014Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Kuniki Kino, Ryotaro Hara
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Publication number: 20130330786Abstract: Development of a method of economically and efficiently producing cis-4-hydroxy-L-proline. The present invention provides L-proline cis-4-hydroxylase. This enzyme may be derived from Lotus corniculatus rhizobia, Mesorhizobium loti or Medicago sativa rhizobia, Sinorhizobium meliloti. The present invention provides a method of producing cis-4-hydroxy-L-proline from L-proline by using this enzyme. The present invention provides a recombinant vector containing a polynucleotide encoding the enzyme and a transformant containing the vector.Type: ApplicationFiled: August 19, 2013Publication date: December 12, 2013Applicant: Kyowa Hakko Bio Co., Ltd.Inventors: Kuniki KINO, Ryotaro HARA
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Patent number: 8541209Abstract: Development of a method of economically and efficiently producing cis-4-hydroxy-L-proline. The present invention provides L-proline cis-4-hydroxylase. This enzyme may be derived from Lotus corniculatus rhizobia, Mesorhizobium loti or Medicago sativa rhizobia, Sinorhizobium meliloti. The present invention provides a method of producing cis-4-hydroxy-L-proline from L-proline by using this enzyme. The present invention provides a recombinant vector containing a polynucleotide encoding the enzyme and a transformant containing the vector.Type: GrantFiled: May 12, 2009Date of Patent: September 24, 2013Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Kuniki Kino, Ryotaro Hara
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Patent number: 7939294Abstract: The present invention provides a protein having dipeptide-synthesizing activity, a DNA encoding the protein, a recombinant DNA containing the DNA, a transformant obtained via transformation with the recombinant DNA, a process for producing a protein having dipeptide-synthesizing activity using the transformant, a process for producing a dipeptide using a protein having dipeptide-synthesizing activity, and a process for producing a dipeptide using a culture or the like of a transformant or a microorganism that produces a protein having dipeptide-synthesizing activity as an enzyme source.Type: GrantFiled: September 25, 2007Date of Patent: May 10, 2011Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Kuniki Kino, Youichi Kotanaka, Makoto Yagasaki
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Publication number: 20110091942Abstract: Development of a method of economically and efficiently producing cis-4-hydroxy-L-proline. The present invention provides L-proline cis-4-hydroxylase. This enzyme may be derived from Lotus corniculatus rhizobia, Mesorhizobium loti or Medicago sativa rhizobia, Sinorhizobium meliloti. The present invention provides a method of producing cis-4-hydroxy-L-proline from L-proline by using this enzyme. The present invention provides a recombinant vector containing a polynucleotide encoding the enzyme and a transformant containing the vector.Type: ApplicationFiled: May 12, 2009Publication date: April 21, 2011Applicant: KYOWA HAKKO BIO CO., LTD.Inventors: Kuniki Kino, Ryotaro Hara
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Patent number: 7871808Abstract: The present invention provides a method for producing an amino acid selected from the group consisting of L-alanine, L-valine, L-leucine, L-isoleucine, L-methionine, L-phenylalanine, L-proline, glycine, L-serine, L-threonine, L-cysteine, L-tyrosine, L-asparagine, L-glutamine, L-lysine, L-histidine, L-arginine, L-aspartic acid and L-glutamic acid and useful as medicament, chemical agent, food material and feed additive at high industrial efficiency, the method comprising culturing a microorganism having an ability to produce the amino acid and having resistance to an aminoquinoline derivative in a medium, producing and accumulating the amino acid in the present invention in the culture, and recovering the amino acid from the culture.Type: GrantFiled: May 1, 2006Date of Patent: January 18, 2011Assignee: Kyowa Hakko Bio Co., Ltd.Inventors: Kuniki Kino, Tetsuya Abe
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Publication number: 20100248307Abstract: According to the present invention, a protein having peptide-synthesizing activity, a DNA encoding the protein, a recombinant DNA containing the DNA, a transformant obtained via transformation with the recombinant DNA, a process for producing a protein having peptide-synthesizing activity using the transformant and the like, a process for producing a peptide using a protein having peptide-synthesizing activity, and a process for producing a peptide using as an enzyme source a culture or the like of a transformant or a microorganism producing a protein having peptide-synthesizing activity are provided.Type: ApplicationFiled: June 19, 2008Publication date: September 30, 2010Applicant: KYOWA HAKKO BIO CO., LTD.Inventors: Kuniki Kino, Toshinobu Arai, Masahiro Kokubo, Makoto Yagasaki
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Publication number: 20100129866Abstract: According to the present invention, a protein having dipeptide-synthesizing activity, a DNA encoding the protein, a recombinant DNA containing the DNA, a transformant obtained via transformation with the recombinant DNA, a process for producing a protein having dipeptide-synthesizing activity using the transformant or the like, a process for producing a dipeptide using a protein having dipeptide-synthesizing activity, and a process for producing a dipeptide using a culture or the like of a transformant or a microorganism that produces a protein having dipeptide-synthesizing activity as an enzyme source are provided.Type: ApplicationFiled: September 25, 2007Publication date: May 27, 2010Applicant: Kyowa Hakko Bio Co., Ltd.Inventors: Kuniki Kino, Youichi Kotanaka, Makoto Yagasaki
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Publication number: 20090197303Abstract: The present invention provides: a protein having dipeptide-synthesizing activity; DNA encoding the protein; a recombinant DNA comprising the DNA; a transformant transformed with the recombinant DNA; a process for producing the protein having dipeptide-synthesizing activity using the transformant or the like; a process for producing a dipeptide using the protein having dipeptide-synthesizing activity; and a process for producing a dipeptide using, as an enzyme source, a culture of a transformant or a microorganism which produces the protein having dipeptide-synthesizing activity or the like.Type: ApplicationFiled: December 27, 2006Publication date: August 6, 2009Applicant: KYOWA HAKKO KOGYO CO., LTD.Inventors: Kuniki Kino, Atsushi Noguchi, Yuji Nakazawa, Makoto Yagasaki