Patents by Inventor Kunio Ohyama
Kunio Ohyama has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 11926845Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.Type: GrantFiled: January 29, 2021Date of Patent: March 12, 2024Assignee: Hitachi, Ltd.Inventors: Yuki Niimi, Hiroko Hanzawa, Maiko Tanabe, Kunio Ohyama, Shizu Takeda
-
Publication number: 20220340847Abstract: The present invention relates to an automatic culture device using a single-use closed system flow path for culturing a cell or a tissue, and realizes reduction in manufacturing cost and high integration property of a device. A cell culture system including an automatic culture device and an information processing device. The automatic culture device includes a plurality of types of closed system flow paths possible to be installed and removed, and a plurality of culture devices.Type: ApplicationFiled: April 14, 2022Publication date: October 27, 2022Inventors: Takayuki NOZAKI, Kunio OHYAMA, Masaharu KIYAMA, Kakuro HIRAI
-
Publication number: 20210301248Abstract: Provided are an evaluator including an analysis unit that evaluates a differentiation level of a cultured cell on the basis of a content of a component in a culture supernatant of the cultured cell, and an automatic cell culture system including the evaluator and an automatic culture device.Type: ApplicationFiled: November 18, 2020Publication date: September 30, 2021Inventors: Hikaru SAITO, Midori KATO, Kakuro HIRAI, Masaharu KIYAMA, Kunio OHYAMA, Hiroko HANZAWA, Shizu TAKEDA
-
Publication number: 20210284959Abstract: A novel cell culture method for inducing differentiation of a pluripotent stem cell into trophoblast and an automatic culture apparatus therefor includes: a first step of culturing the pluripotent stem cell in a presence of a ROCK inhibitor during a first time period; a second step of culturing the pluripotent stem cell, which has been subjected to the first step, without the ROCK inhibitor during a second time period following the first time period; and a step of culturing the pluripotent stem cell, which has been subjected to the second step, in the presence of the ROCK inhibitor during a third time period following the second time period, in which the pluripotent stem cell is cultured in a state of being adhered to a cell culture substrate including a planar mesh through the first to third time periods.Type: ApplicationFiled: January 29, 2021Publication date: September 16, 2021Inventors: Yuki NIIMI, Hiroko HANZAWA, Maiko TANABE, Kunio OHYAMA, Shizu TAKEDA
-
Patent number: 10947490Abstract: A liquid delivery device includes a first and second container for a first and second liquid; a third container for receiving a first and second liquid; a fourth container into which a first and second liquid are discharged; a fifth container for a third liquid; a first liquid delivery tube for delivering a first liquid; a second liquid delivery tube for delivering a second liquid; a third liquid delivery tube connected to the first and second liquid delivery tube; a first liquid delivery pump on the third liquid delivery tube; a fourth liquid delivery tube for discharging a first liquid contained in the third container and delivering a third liquid to the third container; a fifth liquid delivery tube connected to the fourth liquid delivery tube; a sixth liquid delivery tube connected to the fourth liquid delivery tube; and a second liquid delivery pump on the fourth liquid delivery tube.Type: GrantFiled: February 14, 2018Date of Patent: March 16, 2021Assignee: HITACHI, LTD.Inventors: Hikaru Saito, Masaharu Kiyama, Midori Kato, Kunio Ohyama, Guangbin Zhou, Hiroko Hanzawa, Shizu Takeda
-
Publication number: 20210009935Abstract: An object of the invention is to provide a cell culture monitoring device capable of monitoring the number of cells during culture and a cell culture system. The cell culture monitoring device for monitoring proliferation of cells includes: a detection unit configured to detect particles of exosomes in culture supernatant; and an analysis unit configured to calculate the number of cells based on an obtained detection result. The cell culture system includes the cell culture monitoring device and an automatic culture device.Type: ApplicationFiled: July 9, 2020Publication date: January 14, 2021Inventors: Hikaru SAITO, Kakuro HIRAI, Midori KATO, Masaharu KIYAMA, Kunio OHYAMA, Hiroko HANZAWA, Shizu TAKEDA
-
Publication number: 20180362915Abstract: A liquid delivery device includes a first and second container for a first and second liquid; a third container for receiving a first and second liquid; a fourth container into which a first and second liquid are discharged; a fifth container for a third liquid; a first liquid delivery tube for delivering a first liquid; a second liquid delivery tube for delivering a second liquid; a third liquid delivery tube connected to the first and second liquid delivery tube; a first liquid delivery pump on the third liquid delivery tube; a fourth liquid delivery tube for discharging a first liquid contained in the third container and delivering a third liquid to the third container; a fifth liquid delivery tube connected to the fourth liquid delivery tube; a sixth liquid delivery tube connected to the fourth liquid delivery tube; and a second liquid delivery pump on the fourth liquid delivery tube.Type: ApplicationFiled: February 14, 2018Publication date: December 20, 2018Inventors: Hikaru SAITO, Masaharu KIYAMA, Midori KATO, Kunio OHYAMA, Guangbin ZHOU, Hiroko HANZAWA, Shizu TAKEDA
-
Patent number: 4495151Abstract: Apparatus for performing an immunoassay, comprises in combination a reaction tube closed at its bottom, and in the reaction tube a molded element having on its surface material having specific binding ability for a component in a liquid specimen to be assayed. The shape of the surface of the element adjacent the interior surface of the tube is the complement of the shape of the interior surface of the tube. A spacer rib is provided on the element, in contact with the interior surface of the tube. This rib holds the element in such position in the tube that the surfaces of complementary shape of the element and tube are shaped from each other a small but constant distance. A thin layer of constant thickness of a liquid specimen for immunoassay is thus receivable between the complementary surfaces. This arrangement increases the contact surface of the liquid medium in the reaction tube by the element, by establishing a homogeneous uniform layer of liquid medium.Type: GrantFiled: July 1, 1982Date of Patent: January 22, 1985Assignee: Toyo Jozo Kabushiki KaishaInventors: Kunio Ohyama, Nobuaki Nakagawa, Susumu Watanabe
-
Patent number: 4423034Abstract: A novel peptide of the formulaH--Ser--Arg--Arg--Ala--Gln--Asp--Phe--Val--Gln--Trp--Leu--Met--Asn--Thr--OHis the 16-29 fragment of glucagon and can be conjugated with a protein such as albumin or modified albumin using glutaraldehyde or 3-(2'-benzothiazolyl-dithio) propionate succinimide ester as a conjugation reagent. The conjugated peptide can be used to sensitize a mammal by administering the conjugated peptide subcutaneously, after which the blood is collected from the mammal and antibodies concentrated from the blood, which antibodies are then useful in assay methods for trace amounts of glucagon in human blood.Type: GrantFiled: April 28, 1982Date of Patent: December 27, 1983Assignee: Toyo Jozo Kabushiki KaishaInventors: Nobuaki Nakagawa, Kikuo Kotani, Shigeo Katsuragi, Kaoru Morita, Kunio Ohyama, Toshiharu Noda
-
Patent number: 4409141Abstract: A peptide of the formulaR--Lys--Lys--Glu--Asp--Asn--Val--Leu--Val--Glu--Ser--His--Glu--Lys--Ser--Le u--Gly--Glu--Ala--Asp--Lys--Ala--Asp--Val--Asp--Val--Leu--Thr--Lys--Ala--Ly s--Ser--Gln--Ohwherein R is H, H--Tyr-- or R.sub.1 --Pro--Arg--, R.sub.1 is H, H--Tyr-- or R.sub.2 --Ala--Gly--Ser--Gln--Arg--, and R.sub.Type: GrantFiled: December 29, 1981Date of Patent: October 11, 1983Assignee: Toyo Jozo Kabushiki KaishaInventors: Toshiharu Noda, Kaoru Morita, Sadami Kobari, Nobuaki Nakagawa, Susumu Watanabe, Shigeo Katsuragi, Kunio Ohyama, Masahiko Taniuchi
-
Patent number: 4287345Abstract: A polyfunctional disulfide compound, useful as a cross-linking reagent having S--S exchange reactivity, of the formula ##STR1## wherein R is 2-benzothiazolyl or 2-pyridyl-N-oxide and X is a spacer group having an alkylene group directly bonded to each S--S group.Type: GrantFiled: January 11, 1980Date of Patent: September 1, 1981Assignee: Toyo Jozo Kabushiki KaishaInventors: Kikuo Kotani, Kunio Ohyama, Nobuaki Nakagawa, Tadashiro Fujii, Susumu Watanabe
-
Patent number: RE33188Abstract: A peptide of the formulaR--Lys--Lys--Glu--Asp--Asn--Val--Leu--Val--Glu--Ser--His--Glu--Lys--Ser--Le u--Gly--Glu--Ala--Asp--Lys--Ala--Asp--Val--Asp--Val--Leu--Thr--Lys--Ala--Ly s--Ser--Gln--OHwherein R is H, H--Tyr-- or R.sub.1 --Pro--Arg--, R.sub.1 is H, H--Tyr-- or R.sub.2 --Ala--Gly--Ser--Gln--Arg--, and R.sub.2 is H, H--Cys-- or H--Tyr--, or a salt thereof, is useful for assaying human parathyroid hormone (h-PTH).Type: GrantFiled: April 3, 1989Date of Patent: March 27, 1990Assignee: Toyo Jozo Kabushiki KaishaInventors: Toshiharu Noda, Kaoru Morita, Sadami Kobari, Nobuaki Nakagawa, Susumu Watanabe, Shigeo Katsuragi, Kunio Ohyama, Masahiko Taniuchi