Patents by Inventor Kyeong-Sik Shin
Kyeong-Sik Shin has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240060993Abstract: Provided is a method by which a biosensor device detects an antigen by using multiple frequencies, the method enabling an antigen to be detected with high sensitivity. The method by which a biosensor device detects an antigen by using multiple frequencies includes: loading an antibody in the biosensor device including a micro-well that has an action electrode and a counterpart electrode disposed therein; measuring the impedance of the antibody for each of a first frequency and a second frequency; performing an antigen-antibody reaction by injecting an antigen into the biosensor device; using the first frequency and the second frequency to measure the impedances of each of the antigen and the antibody that are bound by means of the antigen-antibody reaction; and calculating changes in impedance that result from the antigen-antibody reaction from the impedances of the antigen and the antibody for the first frequency and the impedances of the antigen and the antibody for the second frequency.Type: ApplicationFiled: December 28, 2021Publication date: February 22, 2024Inventors: Kyeong Sik SHIN, Ji Yoon KANG
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Publication number: 20220307099Abstract: A steel sheet having high strength and high formability according to an aspect of the present invention includes: % by weight, an amount of 0.12-0.22% of carbon (C); an amount of 1.6-2.4% of silicon (Si); an amount of 2.0-3.0% of manganese (Mn); an amount of 0.01-0.05% of aluminum (Al); an amount greater than 0 and less than or equal to 0.05% of the sum of one or more of titanium (Ti), niobium (Nb) and vanadium (V); an amount of 0.015% or less of phosphorus (P); an amount of 0.003% or less of sulfur (S); an amount of 0.006% or less of nitrogen (N); and the reminder of Fe and inevitable impurities, and has a yield strength (YS) of 850 MPa or greater, a tensile strength (TS) of 1180 MPa or greater, an elongation ratio (EL) of 14% or greater, and a hole expansion ratio (HER) or 30% of greater.Type: ApplicationFiled: May 15, 2020Publication date: September 29, 2022Inventors: Gyu Jin Oh, Nam Hoon Goo, Kyeong Sik Shin, Ho Yong Um
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Patent number: 11313828Abstract: The biosensor includes a substrate, an electrode pattern positioned on the substrate, a passivation layer which is formed with a plurality of holes spaced apart from each other, and a bead positioned at one or more holes among the plurality of holes, and to which an antibody is attached, the electrode pattern includes a first electrode pattern part and a second electrode pattern part spaced apart from the first electrode pattern part, which has a same height as a height of the first electrode pattern part, and forms an electric field with the first electrode pattern part.Type: GrantFiled: January 13, 2016Date of Patent: April 26, 2022Assignee: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Ji Yoon Kang, Kyeong-Sik Shin, Jae-hoon Ji, Kyoseon Hwang, Tae Song Kim
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Publication number: 20200188918Abstract: The present invention relates to a method for isolating extracellular vesicles comprising: preparing a microfluidic chip in which at least two microfluidic channels are formed and inlets and outlets are formed at both ends of the channels, respectively; injecting at least one aqueous solution into the inlet of the microfluidic chip to form a microfluid; injecting a sample containing extracellular vesicles into the inlet of the microfluidic chip; and recovering the fluid from the outlet of the microfluidic chip.Type: ApplicationFiled: November 13, 2019Publication date: June 18, 2020Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Ji Yoon KANG, Bo Hoon HAN, You hee HEO, Kyeong Sik SHIN
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Patent number: 10648988Abstract: A method for providing information relevant to the diagnosis of a neurodegenerative disorder. The method includes (i) obtaining a biological sample containing vesicles from a subject, (ii) measuring the level of amyloid beta in the biological sample using an antibody specific for the N-terminus of amyloid beta, and (iii) comparing the measured level of amyloid beta in the biological sample with the level of amyloid beta in a previously prepared control sample.Type: GrantFiled: January 23, 2018Date of Patent: May 12, 2020Assignee: Korea Institute of Science and TechnologyInventors: Ji Yoon Kang, Kyeong-Sik Shin, Jae-hoon Ji, Youhee Heo, Kyoseon Hwang, Myung-Sic Chae
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Patent number: 10585105Abstract: The present invention relates to a method for diagnosing a disease using an analysis of oligomer of an abnormal aggregated protein includes: (1) preparing a body fluid sample including at least one of blood, blood plasma, blood serum, saliva, urine, tear, and mucus; (2) making a dilution of the body fluid sample; (3) using a biosensor to measure and detect an aggregated protein in the diluted body fluid sample; (4) analyzing a signal change of the biosensor caused by the dilution of the aggregated protein to determine a slope according to the dilution from the measurements; and (5) analyzing a proportion of the oligomer from the slope according to the dilution to make a diagnosis. The method uses a biosensor to measure the impedance and the protein concentration of blood and detects the slope according to the numerical value of the monomer and the oligomer to diagnose normal or abnormal protein aggregation or the associated diseases with more accuracy.Type: GrantFiled: May 18, 2016Date of Patent: March 10, 2020Assignee: Korea Institute of Science and TechnologyInventors: Ji Yoon Kang, Kyeong Sik Shin, Jae Hoon Ji, Tae Song Kim, Young Soo Kim, Sang Yun Kim
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Publication number: 20180238910Abstract: A method for providing information relevant to the diagnosis of a neurodegenerative disorder. The method includes (i) obtaining a biological sample containing vesicles from a subject, (ii) measuring the level of amyloid beta in the biological sample using an antibody specific for the N-terminus of amyloid beta, and (iii) comparing the measured level of amyloid beta in the biological sample with the level of amyloid beta in a previously prepared control sample.Type: ApplicationFiled: January 23, 2018Publication date: August 23, 2018Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Ji Yoon KANG, Kyeong-Sik SHIN, Jae-hoon JI, Youhee HEO, Kyoseon HWANG, Myung-Sic CHAE
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Publication number: 20180080946Abstract: The present invention relates to a method for diagnosing a disease using an analysis of oligomer of an abnormal aggregated protein includes: (1) preparing a body fluid sample including at least one of blood, blood plasma, blood serum, saliva, urine, tear, and mucus; (2) making a dilution of the body fluid sample; (3) using a biosensor to measure and detect an aggregated protein in the diluted body fluid sample; (4) analyzing a signal change of the biosensor caused by the dilution of the aggregated protein to determine a slope according to the dilution from the measurements; and (5) analyzing a proportion of the oligomer from the slope according to the dilution to make a diagnosis. The method uses a biosensor to measure the impedance and the protein concentration of blood and detects the slope according to the numerical value of the monomer and the oligomer to diagnose normal or abnormal protein aggregation or the associated diseases with more accuracy.Type: ApplicationFiled: May 18, 2016Publication date: March 22, 2018Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Ji Yoon KANG, Kyeong Sik SHIN, Jae Hoon JI, Tae Song KIM, Young Soo KIM, Sang Yun KIM
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Publication number: 20170131233Abstract: The biosensor includes a substrate, an electrode pattern positioned on the substrate, a passivation layer which is formed with a plurality of holes spaced apart from each other, and a bead positioned at one or more holes among the plurality of holes, and to which an antibody is attached, the electrode pattern includes a first electrode pattern part and a second electrode pattern part spaced apart from the first electrode pattern part, which has a same height as a height of the first electrode pattern part, and forms an electric field with the first electrode pattern part.Type: ApplicationFiled: January 13, 2016Publication date: May 11, 2017Applicant: KOREA INSTITUTE OF SCIENCE AND TECHNOLOGYInventors: Ji Yoon Kang, Kyeong-Sik Shin, Jae-hoon Ji, Kyoseon Hwang, Tae Song Kim
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Patent number: 9645135Abstract: The present invention is directed to a multiwire nanowire field effect transistor (nwFET) device for the measurement. The device includes a sensing nanowire having a first end and a second end and a nanowire FET having a first end and a second end, wherein the first end of the sensing nanowire is connected to the nanowire FET to form a node. Additionally, the first end of the nanowire FET is connected to a source electrode, the second end of the nanowire FET is connected to a drain electrode, and the second end of the sensing nanowire is connected to a base electrode. The sensing nanowire is derivatized with a plurality of immobilized capture probes that are specific for a target(s) of interest. The device is used to detect biomolecules or to detect the change in the ionic environment of a sample. In a further embodiment, the sensing nanowire is derivatized with amino, carboxyl or hydroxyl groups.Type: GrantFiled: December 2, 2011Date of Patent: May 9, 2017Assignee: THE REGENTS OF THE UNIVERSITY OF CALIFORNIAInventors: Kyeong-Sik Shin, Chi On Chui
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Publication number: 20130337567Abstract: The present invention is directed to a multiwire nanowire field effect transistor (nwFET) device for the measurement. The device includes a sensing nanowire having a first end and a second end and a nanowire FET having a first end and a second end, wherein the first end of the sensing nanowire is connected to the nanowire FET to form a node. Additionally, the first end of the nanowire FET is connected to a source electrode, the second end of the nanowire FET is connected to a drain electrode, and the second end of the sensing nanowire is connected to a base electrode. The sensing nanowire is derivatized with a plurality of immobilized capture probes that are specific for a target(s) of interest. The device is used to detect biomolecules or to detect the change in the ionic environment of a sample. In a further embodiment, the sensing nanowire is derivatized with amino, carboxyl or hydroxyl groups.Type: ApplicationFiled: December 2, 2011Publication date: December 19, 2013Inventors: Kyeong-Sik Shin, Chi On Chui