Patents by Inventor Kyle B. Cole

Kyle B. Cole has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Nucleic acid labeling methods
    Patent number: 7824863
    Abstract: In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3?-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3?-AppN-3?-linker-detectable moiety is used as donor molecule.
    Type: Grant
    Filed: October 22, 2008
    Date of Patent: November 2, 2010
    Assignee: Affymetrix, Inc.
    Inventors: Kyle B. Cole, Vivi Truong, Glenn H. McGall, Anthony D. Barone
  • Nucleic acid labeling methods
    Patent number: 7504215
    Abstract: In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3?-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3?-AppN-3?-linker-detectable moiety is used as donor molecule.
    Type: Grant
    Filed: November 4, 2004
    Date of Patent: March 17, 2009
    Assignee: Affymetrix, Inc.
    Inventors: Kyle B. Cole, Vivi Truong, Glenn H. McGall, Anthony D. Barone
  • Nucleic Acid Labeling Methods
    Publication number: 20090054258
    Abstract: In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one aspect of the present invention, T4 RNA ligase is used to attach a 3?-labeled AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3?-AppN-3?-linker-detectable moiety is used as donor molecule.
    Type: Application
    Filed: October 22, 2008
    Publication date: February 26, 2009
    Applicant: Affymetrix, Inc
    Inventors: Kyle B. Cole, Vivi Truong, Glenn H. McGall, Anthony D. Barone
  • Methods for Analyzing Global Regulation of Coding and Non-Coding RNA Transcripts Involving Low Molecular Weight RNAs
    Publication number: 20080261817
    Abstract: In some embodiments of the invention, methods are provided to interrogate the transcriptional activity relating to RNAs of small molecular weight. The methods employ hybridization of a large number of oligonucleotide probes with nucleic acid derived from RNAs of small molecular weight.
    Type: Application
    Filed: October 31, 2006
    Publication date: October 23, 2008
    Applicant: Affymetrix, INC.
    Inventors: Philipp Kapranov, Dione Kampa, Thomas R. Gingeras, Stefan Bekiranov, Simon Cawley, Kyle B. Cole
  • Methods for reducing background on polynucleotide arrays
    Publication number: 20040234964
    Abstract: Methods are provided for reducing background signal associated with hybridization of nucleic acid arrays to nucleic acid samples, the method comprising hybridizing the array to the sample in the presence of a poly-anionic polymer (PAP). Background associated with hybridization of arrays to samples interferes with signal generated by specific binding to the probe array un-desirably lowers the signal to noise ratio (SNR) and generates an overall loss of sensitivity for nucleic acid arrays.
    Type: Application
    Filed: May 19, 2003
    Publication date: November 25, 2004
    Applicant: Affymetrix, INC.
    Inventors: Kyle B. Cole, Vivi Truong, Glenn McGall, Sumathi Venkatapathy
  • Methods for analyzing global regulation of coding and non-coding RNA transcripts involving low molecular weight RNAs
    Publication number: 20040180364
    Abstract: In some embodiments of the invention, methods are provided to interrogate the transcriptional activity relating to RNAs of small molecular weight. The methods employ hybridization of a large number of oligonucleotide probes with nucleic acid derived from RNAs of small molecular weight.
    Type: Application
    Filed: December 15, 2003
    Publication date: September 16, 2004
    Applicant: Affymetrix, INC.
    Inventors: Philipp Kapranov, Dione Kampa, Thomas R. Gingeras, Stefan Bekiranov, Simon Cawley, Kyle B. Cole
  • Nucleic acid labeling methods
    Publication number: 20040086914
    Abstract: In one aspect of the invention, a method is provided for end-labeling RNA (total RNA, mRNA, cRNA or fragmented RNA). In one embodiment, T4 RNA ligase is used to attach a 3′-biotinylated AMP or CMP donor to an RNA acceptor molecule. In another embodiment, a pyrophosphate molecule 3′-AppN-3′-linker-biotin is used as donor molecule.
    Type: Application
    Filed: July 11, 2003
    Publication date: May 6, 2004
    Applicant: Affymetrix, INC.
    Inventors: Kyle B. Cole, Vivi Truong, Glenn H. McGall, Anthony D. Barone
  • Methods for screening polypeptides
    Publication number: 20030087232
    Abstract: In one aspect of the invention, methods are provided for the creation and screening of polypeptides that eliminates bacterial cloning and individual screening. In preferred embodiments, the method involves partnering each protein with a unique DNA oligonucleotide tag that directs the protein to a unique site on the microarray due to specific hybridization with a complementary tag-probe on the array.
    Type: Application
    Filed: January 24, 2002
    Publication date: May 8, 2003
    Inventors: Fred Christians, Kyle B. Cole