Patents by Inventor Kyle R. Gee
Kyle R. Gee has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10550422Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation.Type: GrantFiled: October 24, 2016Date of Patent: February 4, 2020Assignees: President and Fellows of Harvard College, Life Technologies CorporationInventors: Kyle R. Gee, Brian Agnew, Adrian Salic, Timothy J. Mitchison
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Publication number: 20170137866Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation.Type: ApplicationFiled: October 24, 2016Publication date: May 18, 2017Inventors: Kyle R. Gee, Brian Agnew, Adrian Salic, Timothy J. Mitchison
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Patent number: 9512465Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation.Type: GrantFiled: August 21, 2013Date of Patent: December 6, 2016Assignees: Life Technologies Corporation, President and Fellows of Harvard CollegeInventors: Kyle R. Gee, Brian Agnew, Adrian Salic, Timothy J. Mitchison
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Patent number: 8716033Abstract: The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.Type: GrantFiled: February 12, 2007Date of Patent: May 6, 2014Assignee: Life Technologies CorporationInventors: Brian Agnew, Schuyler B. Corry, Kyle R. Gee
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Publication number: 20140065605Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation.Type: ApplicationFiled: August 21, 2013Publication date: March 6, 2014Applicants: Life Technologies Corporation, President and Fellows of Harvard CollegeInventors: Kyle R. Gee, Brian Agnew, Adrian Salic, Timothy J. Mitchison
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Patent number: 8541570Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation.Type: GrantFiled: May 24, 2010Date of Patent: September 24, 2013Assignees: President and Fellows of Harvard College, Life Technologies CorporationInventors: Kyle R. Gee, Brian Agnew, Adrian Salic, Timothy J. Mitchison
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Publication number: 20120301894Abstract: Provided in certain embodiments are new methods for forming azido modified biomolecule conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling a biomolecules with an azide group.Type: ApplicationFiled: February 17, 2012Publication date: November 29, 2012Applicant: Life Technologies CorporationInventors: Brian AGNEW, Kyle R. Gee, Tamara G. Nyberg
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Patent number: 8114636Abstract: Provided in certain embodiments are new methods for forming azido modified nucleic acid conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling nucleic acids with an azide group.Type: GrantFiled: February 13, 2007Date of Patent: February 14, 2012Assignee: Life Technologies CorporationInventors: Brian Agnew, Maura J. Ford, Kyle R. Gee, Kapil Kumar
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Publication number: 20110207171Abstract: The present invention generally relates to methods of functionalizing proteins, particularly antibodies, at oligosaccharide linkages, methods of humanizing antibodies by modifying glycosylation, as well as to novel antibodies linked to modified oligosaccharides. The invention further relates to kits that may be used to produce the antibodies of the invention.Type: ApplicationFiled: January 14, 2011Publication date: August 25, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Brian Agnew, Schuyler B. CORRY, Kyle R. GEE
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Publication number: 20110159517Abstract: The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of zinc ions in a sample. The metal chelating moiety of the zinc-binding compound is an analog of the well-known calcium chelator, BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N?,N?-tetraacetic acid), wherein the chelating moiety has been modified from a tetraacetic acid moiety to a tri- di- or monoacetic moiety. This change in acetic acid groups on the metal chelating moiety results in the selective bindings of zinc ions in the presence of calcium ions, both of which are present in biological fluids and intracellular cytosolic fluid and organelles.Type: ApplicationFiled: February 18, 2011Publication date: June 30, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventor: Kyle R. GEE
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Publication number: 20100311063Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation.Type: ApplicationFiled: May 24, 2010Publication date: December 9, 2010Inventors: Kyle R. Gee, Brian Agnew, Adrian Salic, Timothy J. Mitchison
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Publication number: 20100261181Abstract: Provided in certain embodiments are new methods for forming azido modified nucleic acid conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling nucleic acids with an azide group.Type: ApplicationFiled: March 23, 2010Publication date: October 14, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Brian Agnew, Maura J. Ford, Kyle R. Gee, Kapil Kumar
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Patent number: 7776533Abstract: The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.Type: GrantFiled: October 24, 2006Date of Patent: August 17, 2010Assignee: Life Technologies CorporationInventors: Brian Agnew, Joseph Beechem, Kyle R Gee, Richard P Haugland, Thomas H Steinberg, Wayne F Patton
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Patent number: 7767421Abstract: The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation.Type: GrantFiled: October 27, 2006Date of Patent: August 3, 2010Assignees: President and Fellows of Harvard College, Life Technologies CorporationInventors: Kyle R. Gee, Brian Agnew, Adrian Salic, Timothy J. Mitchison
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Publication number: 20100167333Abstract: The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample.Type: ApplicationFiled: December 11, 2009Publication date: July 1, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kyle R. Gee, Jolene Bradford, Vladimir V. Martin
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Publication number: 20100047821Abstract: The invention describes crown ether chelators, including crown ethers having the formula:Type: ApplicationFiled: August 24, 2009Publication date: February 25, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kyle R. Gee, Vladimir V. Martin
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Patent number: 7579463Abstract: The invention describes crown ether chelators, including crown ethers having the formula:Type: GrantFiled: October 2, 2006Date of Patent: August 25, 2009Assignee: Life Technologies CorporationInventors: Kyle R. Gee, Vladimir V. Martin
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Publication number: 20090181364Abstract: The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of zinc ions in a sample. The metal chelating moiety of the zinc-binding compound is an analog of the well-known calcium chelator, BAPTA (1,2-bis(2-aminophenoxy)ethane-N,N,N?,N?-tetraacetic acid), wherein the chelating moiety has been modified from a tetraacetic acid moiety to a tri- di- or monoacetic moiety. This change in acetic acid groups on the metal chelating moiety results in the selective bindings of zinc ions in the presence of calcium ions, both of which are present in biological fluids and intracellular cytosolic fluid and organelles.Type: ApplicationFiled: July 2, 2008Publication date: July 16, 2009Applicant: INVITROGEN CORPORATIONInventor: Kyle R. Gee
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Patent number: 7521577Abstract: The present invention provides a metal chelator and methods that facilitate binding, detecting, monitoring and quantitating of heavy metal ions in a sample.Type: GrantFiled: January 12, 2007Date of Patent: April 21, 2009Assignee: Molecular Probes, Inc.Inventors: Kyle R. Gee, Jolene Bradford, Vladimir V. Martin
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Patent number: 7445894Abstract: The present invention relates to phosphate-binding compounds that find use in binding, detecting and isolating phosphorylated target molecules including the subsequent identification of target molecules that interact with phosphorylated target molecules or molecules capable of being phosphorylated. A binding solution is provide that comprises a phosphate-binding compound, an acid and a metal ion wherein the metal ion simultaneously interacts with an exposed phosphate group on a target molecule and the metal chelating moiety of the phosphate-binding compound forming a bridge between the phosphate-binding compound and a phosphorylated target molecule resulting in a ternary complex. The binding solution of the present invention finds use in binding and detecting immobilized and solubilized phosphorylated target molecules, isolation of phosphorylated target molecules from a complex mixture and aiding in proteomic analysis wherein kinase and phosphatase substrates and enzymes can be identified.Type: GrantFiled: April 9, 2004Date of Patent: November 4, 2008Assignee: Molecular Probes, Inc.Inventors: Brian Agnew, Kyle R. Gee, Vladimir V. Martin