Patents by Inventor Kyung-Han Han

Kyung-Han Han has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Isopentenyl diphosphate isomerase from Hevea brasiliensis and rubber producing method using the same
    Patent number: 6316695
    Abstract: The present invention cloned a cDNA clone encoding isopentenyl diphosphate (hereafter “IPP”) isomerase (EC 5.3.3.2) from a cDNA library of Hevea brasiliensis latex. The clone has a continuous open reading frame encoding a peptide of 234 amino acids with a predicted molecular mass of 26.7 kDa. The deduced protein is acidic with an isoelectric point of 4.7 and shows high sequence identity with other IPP isomerases. The recombinant protein expressed in Escherichia coli showed IPP isomerase activity. In vitro rubber biosynthesis assays using washed rubber particle (WRP) deprived of initiating allylic diphosphates were performed with the addition of IPP isomerase in the reaction mixture. Results revealed that the recombinant IPP isomerase is catalytically active in catalyzing the conversion of IPP to DMAPP, a key activation step of the basic five-carbon isoprene unit in rubber biosynthesis. Southern analysis indicated that the IPP isomerase is encoded by two genes in Hevea rubber tree.
    Type: Grant
    Filed: April 22, 1999
    Date of Patent: November 13, 2001
    Assignee: Korea Kumho Petrochemical Co., Ltd.
    Inventors: Kyung-Han Han, Hun-Seung Kang, Soo-Kyung Oh, Dong-Ho Shin, Jae-Mo Yang