Patents by Inventor Lakmal Nishantha Jayasinghe
Lakmal Nishantha Jayasinghe has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20240117421Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.Type: ApplicationFiled: January 26, 2023Publication date: April 11, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Yayne Wallace
-
Publication number: 20240117422Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.Type: ApplicationFiled: May 5, 2023Publication date: April 11, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace
-
Patent number: 11920193Abstract: Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.Type: GrantFiled: June 6, 2019Date of Patent: March 5, 2024Assignee: Oxford Nanopore Technologies PLCInventors: James Anthony Clarke, James White, Richard Muscat, Jessica Mary May Johnson, Ramiz Iqbal Nathani, Andrew John Heron, Mark John Bruce, Lakmal Nishantha Jayasinghe, Domenico Caprotti, David Jackson Stoddart, Rebecca Victoria Bowen, Christopher James Wright, Paul Richard Moody
-
Publication number: 20240060126Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein.Type: ApplicationFiled: June 27, 2023Publication date: February 22, 2024Applicant: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Clive Gavin Brown, James White, Andrew John Heron, Mark John Bruce, Christopher Peter Youd, Rebecca Victoria Bowen
-
Publication number: 20230295715Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.Type: ApplicationFiled: January 26, 2023Publication date: September 21, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Yayne Wallace
-
Publication number: 20230272017Abstract: Provided herein relate to modified or mutant forms of secretin and compositions comprising the same. In particular, the modified or mutant forms of secretin permits efficient capture and/or translocation of an analyte through the modified or mutant secretin nanopores. Methods for using unmodified secretin or the modified or mutant forms of secretin and compositions, for example, for characterizing an analyte, e.g., a target polynucleotide, are also provided.Type: ApplicationFiled: October 11, 2022Publication date: August 31, 2023Applicant: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh
-
Patent number: 11739377Abstract: The invention relates to improving the movement of a target polynucleotide with respect to a transmembrane pore when the movement is controlled by a polynucleotide binding protein. The invention also relates to improved transmembrane pores and polynucleotide binding proteins.Type: GrantFiled: October 20, 2020Date of Patent: August 29, 2023Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Jonathan Bankes Pugh, Richard George Hambley, Neil Roger Wood, Clive Gavin Brown, James White, Andrew John Heron, Mark Bruce, Christopher Peter Youd, Rebecca Victoria Bowen
-
Patent number: 11685949Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterization using CsgG.Type: GrantFiled: March 8, 2021Date of Patent: June 27, 2023Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace
-
Publication number: 20230079731Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: ApplicationFiled: September 16, 2022Publication date: March 16, 2023Applicants: VIB VZW, VRIJE UNIVERSITEIT BRUSSEL, OXFORD NANOPORE TECHNOLOGIES PLCInventors: Han Remaut, Sander Egbert Van Der Verren, Nani Van Gerven, Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Robert Jordan, John Joseph Kilgour
-
Patent number: 11597970Abstract: The invention relates to mutant forms of CsgG. The invention also relates to analyte detection and characterisation using CsgG.Type: GrantFiled: March 29, 2021Date of Patent: March 7, 2023Assignee: Oxford Nanopore Technologies PLCInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace
-
Patent number: 11572387Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: GrantFiled: July 2, 2018Date of Patent: February 7, 2023Assignees: VIB VZW, Vrije Universiteit Brussel, Oxford Nanopore Technologies PLCInventors: Han Remaut, Sander Egbert Van Der Verren, Nani Van Gerven, Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Robert Jordan, John Joseph Kilgour
-
Patent number: 11466317Abstract: Methods of characterizing an analyte using a nanopore. One aspect features methods for characterizing a double-stranded polynucleotide using a nanopore, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.Type: GrantFiled: November 29, 2017Date of Patent: October 11, 2022Assignee: Oxford Nanopore Technologies PLCInventors: James Clarke, James White, Richard Muscat, Jessica Mary May Knott, Ramiz Iqbal Nathani, Andrew John Heron, Mark John Bruce, Lakmal Nishantha Jayasinghe, Domenico Caprotti, David Jackson Stoddart, Rebecca Victoria Bowen, Christopher James Wright, Paul Richard Moody
-
Publication number: 20220162568Abstract: A modified portal protein of a bacteriophage DNA packaging motor, wherein the modified portal protein is capable of direct insertion into a membrane and wherein the portal protein is modified compared to the wild type portal protein such that one or more amino acid residues on the outer surface of the portal protein is substituted by one or more other amino acid residue, and/or wherein a one or more amino acid residue is inserted on the outer surface of the portal protein so as to alter the outer surface hydrophobicity of the modified portal protein compared to the wild type portal protein.Type: ApplicationFiled: April 9, 2020Publication date: May 26, 2022Applicants: Oxford Nanopore Technologies Limited, P&Z Biological TechnologyInventors: Farzin Haque, Shaoying Wang, Lakmal Nishantha Jayasinghe, Michael Jordan
-
Publication number: 20220056517Abstract: A system for characterising a target polynucleotide, the system comprising a membrane and a pore complex; wherein the pore complex comprises: (i) a nanopore located in the membrane, and (ii) an auxiliary protein or peptide attached to the nanopore; wherein the nanopore and the auxiliary protein or peptide together form a continuous channel across the membrane, the channel comprising a first constriction region and a second constriction region; wherein the first constriction region is formed by a portion of the nanopore, and wherein the second constriction region is formed by at least a portion of the auxiliary protein or peptide.Type: ApplicationFiled: November 7, 2019Publication date: February 24, 2022Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Han Remaut, Sander Van Der Verren, Nani Van Gerven, Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Robert Jordan, John Joseph Kilgour, Andrew John Heron
-
Publication number: 20220024985Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: ApplicationFiled: July 2, 2018Publication date: January 27, 2022Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Han Remaut, Sander Egbert Van Der Verren, Nani Van Gerven, Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Robert Jordan, John Joseph Kilgour
-
Publication number: 20220024994Abstract: Provided is a method of characterising a polynucleotide using a transmembrane pore, wherein the pore is a double pore comprising a first Csg G pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof.Type: ApplicationFiled: May 3, 2018Publication date: January 27, 2022Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Jordan, Han Remaut
-
Publication number: 20210363577Abstract: Methods of characterizing an analyte using a detector such as a nanopore and an enzyme are provided. One aspect features methods for characterizing a double-stranded polynucleotide using a detector, e.g., without using a hairpin connecting a template and a complement of the double-stranded polynucleotide. Another aspect features methods for characterizing an analyte using a tag-modified nanopore with increased sensitivity and/or higher throughput. Compositions and systems including, e.g., adaptors for attachment to double-stranded polynucleotides and tag-modified nanopores, which can be used in the methods are also provided.Type: ApplicationFiled: June 6, 2019Publication date: November 25, 2021Applicant: Oxford Nanopore Technologies LimitedInventors: James Anthony Clarke, James White, Richard Muscat, Jessica Mary May Johnson, Ramiz Iqbal Nathani, Andrew John Heron, Mark John Bruce, Lakmal Nishantha Jayasinghe, Domenico Caprotti, David Jackson Stoddart, Rebecca Victoria Bowen, Christopher James Wright, Paul Richard Moody
-
Publication number: 20210147486Abstract: The present invention relates to novel protein pores and their uses in analyte detection and characterisation. The invention particularly relates to an isolated pore complex formed by a CsgG-like pore and a modified CsgF peptide, or a homologue or mutant thereof, thereby incorporating an additional channel constriction or reader head in the nanopore. The invention further relates to a transmembrane pore complex and methods for production of the pore complex and for use in molecular sensing and nucleic acid sequencing applications.Type: ApplicationFiled: July 2, 2018Publication date: May 20, 2021Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Han Remaut, Sander Egbert Van Der Verren, Nani Van Gerven, Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Robert Jordan, John Joseph Kilgour
-
Publication number: 20210147490Abstract: Provided is a method of characterising a polynucleotide using a transmembrane pore, wherein the pore is a double pore comprising a first Csg G pore, or a homologue thereof, and a second CsgG pore, or a homologue thereof.Type: ApplicationFiled: May 3, 2018Publication date: May 20, 2021Applicants: Oxford Nanopore Technologies Limited, VIB VZW, Vrije Universiteit BrusselInventors: Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace, Pratik Raj Singh, Richard George Hambley, Michael Jordan, Han Remaut
-
Patent number: 10976300Abstract: Provided herein relate to modified or mutant forms of cytolysin A (ClyA) and compositions comprising the same. In particular, the modified or mutant forms of ClyA permits efficient capture and/or translocation of a target negative-charged molecule or polymer through the modified or mutant ClyA nanopores at low or physiological ionic strengths. Thus, methods for using the modified or mutant forms of ClyA and compositions, for example, for characterizing a target negatively-charged analyte, e.g., a target polynucleotide, are also provided.Type: GrantFiled: December 8, 2016Date of Patent: April 13, 2021Assignee: Katholieke Universiteit LeuvenInventors: Giovanni Maglia, Lorenzo Franceschini, Tine Brouns, Andrew John Heron, Lakmal Nishantha Jayasinghe, Elizabeth Jayne Wallace