Abstract: A dental floss holder comprising a holding portion, and a head portion disposed on the upper end thereof and bent obliquely upward from the holding portion. The head portion has first and second arm portions which symmetrically extend from the lower end of the head portion to both sides. Third and fourth arm portions symmetrically extend from the upper end of the head portion to both sides. First and second spaces for tensioning first and second segments of dental floss are formed between tail ends of the first and third arm portions, and the third and fourth arm portions, respectively. A third space for tensioning a third segment of dental floss is formed between tail ends of the fourth and second arm portions. The first and third segments of floss are parallel to the lengthwise direction of the holding portion, with the second segment of floss perpendicular thereto.