Patents by Inventor Laurent Boitard
Laurent Boitard has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9410151Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.Type: GrantFiled: March 26, 2014Date of Patent: August 9, 2016Assignee: Raindance Technologies, Inc.Inventors: Darren R. Link, Laurent Boitard, Jeffrey Branciforte, Yves Charles, Gilbert Feke, John Q. Lu, David Marran, Ahmadali Tabatabai, Michael Weiner, Wolfgang Hinz, Jonathan M. Rothberg
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Publication number: 20160168524Abstract: The present invention relates to a novel method for cultivating micro-organisms by confinement in micro-bioreactors. Said method comprises using a capillary tube in which a carrier fluid for moving a train of droplets forward flows, said capillary tube comprising micro-bioreactors in which the culture of said micro-organisms takes place, wherein said micro-bioreactors are separated by a spacing fluid which is a gas. The diameter of the micro-bioreactors (5) is smaller than that of the capillary tube (8) and the size of the bubble (6) of said spacing fluid is within a range of two to ten times the diameter of said capillary tube (8). The method can be used for cultivating micro-organisms such as thread-like fungi or planktonic algae.Type: ApplicationFiled: July 10, 2014Publication date: June 16, 2016Applicants: ETABLISSEMENTS J. SOUFFLET, ECOLE SUPERIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARISInventors: Jairo Ivan GARNICA RODRIGUEZ, Laurent BOITARD, Antoine Serge Dominique DREVELLE, Jérôme BIBETTE
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Patent number: 9327242Abstract: This method comprises bringing drops to be separated into contact with an interface (40) suitable for allowing an osmotic equilibrium between the content of each drop to be separated. The method comprises an osmotic flow between the drops (20A) of the first group of drops through the interface (40) in order to modify the density of each drop (20A) of the first group of drops and the separation of the drops (20A, 20B) according to the density thereof or a combination of the density and the volume in order to isolate the drops (20A) of the first group of drops from the drops (20B) of a second group of drops.Type: GrantFiled: March 21, 2012Date of Patent: May 3, 2016Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (C.N.R.S.), UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6), ECOLE NORMALE SUPERIEURE DE LYON, UNIVERSITE PARIS DIDEROT—PARIS 7Inventors: Gaël Yvert, Jérôme Bibette, Jean-Marie Baudry, Laurent Boitard, Nicolas Bremond, Denis Cottinet
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Patent number: 9328344Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.Type: GrantFiled: February 5, 2013Date of Patent: May 3, 2016Assignee: Raindance Technologies, Inc.Inventors: Darren Link, Laurent Boitard, Jeffrey Branciforte, Yves Charles, Gilbert Feke, John Q. Lu, David Marran, Ahmadali Tabatabai, Michael Weiner, Wolfgang Hinz, Jonathan M. Rothberg
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Publication number: 20150087008Abstract: A method for monitoring a reaction and a reaction system are provided. The reaction system includes at least one vessel for the reaction medium, which is in fluid communication with an injection tube; at least one vessel for a carrier fluid that is immiscible with the reaction medium, which is in fluid communication with a reaction tube; the injection tube being mounted so as to lead into the reaction tube such that individual drops of the reaction medium can be injected into the reaction tube and into the immiscible carrier fluid, so as to form a train of reaction chambers; at least one detector for monitoring a reaction; a means for classifying the reaction chambers; and at least one means for recirculating reaction chambers in front of at least one detector for monitoring a reaction.Type: ApplicationFiled: October 2, 2014Publication date: March 26, 2015Inventors: Jérôme Bibette, Pascal Panizza, Nicolas Bremond, Fabien Bertholle, Jean Marie Baudry, Larysa Baraban, Laurent Boitard
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Publication number: 20140323317Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.Type: ApplicationFiled: April 9, 2014Publication date: October 30, 2014Applicant: RAINDANCE TECHNOLOGIES, INC.Inventors: Darren R. Link, Laurent Boitard, Jeffrey Branciforte, Yves Charles, Gilbert Feke, John Q. Lu, David Marran, Ahmadali Tabatabai, Michael Weiner, Wolfgang Hinz, Jonathan M. Rothberg
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Publication number: 20140256595Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. The invention provides for aqueous based emulsions containing uniquely labeled cells, enzymes, nucleic acids, etc., wherein the emulsions further comprise primers, labels, probes, and other reactants. An oil based carrier-fluid envelopes the emulsion library on a microfluidic device, such that a continuous channel provides for flow of the immiscible fluids, to accomplish pooling, coalescing, mixing, sorting, detection, etc., of the emulsion library.Type: ApplicationFiled: March 26, 2014Publication date: September 11, 2014Applicant: RAINDANCE TECHNOLOGIES, INC.Inventors: Darren R. Link, Laurent Boitard, Jeffrey Branciforte, Yves Charles, Gilbert Feke, John Q. Lu, David Marran, Ahmadali Tabatabai, Michael Weiner, Wolfgang Hinz, Jonathan M. Rothberg
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Patent number: 8798341Abstract: A method for reading an emulsion (3) including droplets and a continuous phase surrounding the droplets, the method includes: two-dimensional scanning of the emulsion (3), and construction of a two-dimensional image of the emulsion (3) based on the scanning. Preferably, the droplets do not move during scanning, for example by solidifying the continuous phase or by using a two-dimensional compact or semi-compact network of droplets. The method can further include time-based monitoring of a chemical or biological reaction taking place in at least one of the droplets. A device implementing this method is also described. The method is applicable for the detection and/or sorting of microdroplets performing the role of microreactors or containing specific cells or molecules, in fields such as gene expression or diagnosis.Type: GrantFiled: July 13, 2009Date of Patent: August 5, 2014Assignees: Universite Pierre et Marie Curie (Paris 6), Centre National de la Recherche Scientifique, Universite Paris Diderot-Paris 7Inventors: Jean Baudry, Julien Sylvestre, Jerome Bibette, Laurent Boitard, Nicolas Bremond
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Publication number: 20140069865Abstract: This method comprises bringing drops to be separated into contact with an interface (40) suitable for allowing an osmotic equilibrium between the content of each drop to be separated. The method comprises an osmotic flow between the drops (20A) of the first group of drops through the interface (40) in order to modify the density of each drop (20A) of the first group of drops and the separation of the drops (20A, 20B) according to the density thereof or a combination of the density and the volume in order to isolate the drops (20A) of the first group of drops from the drops (20B) of a second group of drops.Type: ApplicationFiled: March 21, 2012Publication date: March 13, 2014Applicants: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (C.N.R.S.), UNIVERSITE PARIS DIDEROT-PARIS 7, ECOLE NORMALE SUPERIEURE DE LYON, UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6)Inventors: Gaël Yvert, Jérôme Bibette, Jean-Marie Baudry, Laurent Boitard, Nicolas Bremond, Denis Cottinet
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Publication number: 20130217583Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.Type: ApplicationFiled: February 5, 2013Publication date: August 22, 2013Inventors: Darren Link, Laurent Boitard, Jeffrey Branciforte, Yves Charles, Gilbert Feke, John Q. Lu, David Marran, Ahmadali Tabatabai, Michael Weiner, Wolfgang Hinz, Jonathan M. Rothberg
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Publication number: 20110188717Abstract: A method for reading an emulsion (3) including droplets and a continuous phase surrounding the droplets, the method includes: two-dimensional scanning of the emulsion (3), and construction of a two-dimensional image of the emulsion (3) based on the scanning. Preferably, the droplets do not move during scanning, for example by solidifying the continuous phase or by using a two-dimensional compact or semi-compact network of droplets. The method can further include time-based monitoring of a chemical or biological reaction taking place in at least one of the droplets. A device implementing this method is also described. The method is applicable for the detection and/or sorting of microdroplets performing the role of microreactors or containing specific cells or molecules, in fields such as gene expression or diagnosis.Type: ApplicationFiled: July 13, 2009Publication date: August 4, 2011Applicants: UNIVERSITE PIERRE ET MARIE CURIE (PARIS 6), CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE PARIS DIDEROT-PARIS-7Inventors: Jean Baudry, Julien Sylvestre, Jerome Bibette, Laurent Boitard, Nicolas Bremond
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Publication number: 20100137163Abstract: The present invention provides novel microfluidic devices and methods that are useful for performing high-throughput screening assays and combinatorial chemistry. Such methods can include labeling a library of compounds by emulsifying aqueous solutions of the compounds and aqueous solutions of unique liquid labels on a microfluidic device, which includes a plurality of electrically addressable, channel bearing fluidic modules integrally arranged on a microfabricated substrate such that a continuous channel is provided for flow of immiscible fluids, whereby each compound is labeled with a unique liquid label, pooling the labeled emulsions, coalescing the labeled emulsions with emulsions containing a specific cell or enzyme, thereby forming a nanoreactor, screening the nanoreactors for a desirable reaction between the contents of the nanoreactor, and decoding the liquid label, thereby identifying a single compound from a library of compounds.Type: ApplicationFiled: June 1, 2006Publication date: June 3, 2010Inventors: Darren R. Link, Laurent Boitard, Jeffrey Branciforte, Yves Charles, Gilbert Feke, John Q. Lu, David Marran, Ahmadali Tabatabai, Michael Weiner, Wolfgang Hinz, Jonathan M. Rothberg