Abstract: The present disclosure relates to methods of decreasing lactate production in cell culture using divalent transitional metallic salts. The present disclosure also relates to a method of producing polypeptide by adding divalent transitional metallic salt to the cell culture medium for reducing lactate accumulation followed by fermenting and recovering the polypeptide.

Abstract: An automatic syringe pump is disclosed that includes at least four subsystems: mechanical power, electronic timing, mechanical power transfer, and user interface subsystems. In some embodiments, an occlusion detection subsystem is also present. The mechanical power subsystem's main includes a constant force spring capable of storing the energy needed to reliable depressed several different sizes of a syringe. The electronic timing subsystem may include components including a ratchet, pawls, flippers, stepper motor, and microcontroller. These components are used together to regulated the release of the energy stored in the constant force spring. The mechanical power transfer subsystem may include components including a rack and pinion which translates the rotational motion of the drive shaft from the constant force spring into linear motion to depress the syringe. Finally, the user interface subsystem may include components including the microcontroller, Arduino backpack, and syringe tray.

Abstract: The present disclosure relates to methods of decreasing lactate production in cell culture using divalent transitional metallic salts. The present disclosure also relates to a method of producing polypeptide by adding divalent transitional metallic salt to the cell culture medium for reducing lactate accumulation followed by fermenting and recovering the polypeptide.