Abstract: Chimeric polypeptides including (a) an extracellular targeting domain; (b) a transmembrane domain; (c) an intracellular linker for activation of T cells (LAT) domain or SLP-76 domain; and (d) an intracellular ZAP70 domain, wherein (a)-(d) are in N-terminal to C-terminal order are provided. Chimeric polypeptides including (a) an extracellular targeting domain; (b) a transmembrane domain; and (c) a ZAP70 domain, wherein (a)-(c) are in N-terminal to C-terminal order are also provided. In some embodiments, the chimeric polypeptide further includes a hinge domain, a signal sequence domain, and/or an intracellular signaling domain. Nucleic acid molecules encoding the chimeric polypeptides and expression vectors including the nucleic acids are also provided. Isolated cells (such as T cells or natural killer cells) expressing the chimeric polypeptides and methods of treating a subject with cancer with the isolated cells are provided.

Abstract: Fiducial marker compositions comprising fluorescent nanodiamonds and methods of making and using the fiducial marker compositions are disclosed. The fiducial marker composition comprises a substrate, and a fluorescent nanodiamond immobilized on a surface of the substrate, wherein the substrate and immobilized fluorescent nanodiamond are optionally top coated with an inert top coating. The fiducial marker compositions are used in imaging methods to correct for drift and other alignment instabilities, and are particularly useful in super-resolution imaging.