Abstract: The disclosure pertains to materials and methods for capturing target genomic regions, comprising cleaving the target genomic region using endonucleases at specific recognition sites having a minimum of between about 10 and about 30 nucleotides and capturing the target genomic region by hybridizing to a bridge oligonucleotide. The disclosure also pertains to analyzing the captured target genomic regions. The endonucleases used for cleavage can be programmable endonucleases that specifically bind to the recognition sites to direct the cleavage. The captured target genomic regions can be amplified, preferably, via polymerase chain reaction or rolling circle amplification and detected or sequenced. Further, the invention pertains to kits for performing the methods of the invention, comprising one or more endonucleases designed to cleave one or more target genomic regions and bridge oligonucleotides designed to capture the one or more target genomic regions.

Abstract: The disclosure pertains to materials and methods for capturing a target genomic region, comprising hybridizing an extension probe and a ligation probe to target sequences that flank the target genomic region; elongating the 3? end of the extension probe until the 3? end of the elongated extension probe is adjacent to the 5? end of the ligation probe; and ligating the 3? end of the elongated extension probe with the 5? end of the ligation probe to produce a ligated probe. The ligated probe can be PCR amplified to produce copies of the target genomic region that can be detected or sequenced. Certain embodiments of the invention also provide methods of producing double stranded probes suitable for capturing and analyzing both strands of a target genomic region in a double stranded genomic DNA. The invention also provides kits for performing the methods disclosed herein.

Abstract: The disclosure pertains to materials and methods for capturing a target genomic region, comprising hybridizing an extension probe and a ligation probe to target sequences that flank the target genomic region; elongating the 3? end of the extension probe until the 3? end of the elongated extension probe is adjacent to the 5? end of the ligation probe; and ligating the 3? end of the elongated extension probe with the 5? end of the ligation probe to produce a ligated probe. The ligated probe can be PCR amplified to produce copies of the target genomic region that can be detected or sequenced. Certain embodiments of the invention also provide methods of producing double stranded probes suitable for capturing and analyzing both strands of a target genomic region in a double stranded genomic DNA. The invention also provides kits for performing the methods disclosed herein.

Abstract: The disclosure pertains to materials and methods for capturing a target genomic region, comprising hybridizing an extension probe and a ligation probe to target sequences that flank the target genomic region; elongating the 3? end of the extension probe until the 3? end of the elongated extension probe is adjacent to the 5? end of the ligation probe; and ligating the 3? end of the elongated extension probe with the 5? end of the ligation probe to produce a ligated probe. The ligated probe can be PCR amplified to produce copies of the target genomic region that can be detected or sequenced. Certain embodiments of the invention also provide methods of producing double stranded probes suitable for capturing and analyzing both strands of a target genomic region in a double stranded genomic DNA. The invention also provides kits for performing the methods disclosed herein.

Abstract: The present invention provides methods to produce a reduced representation of a genome for sequencing and DNA polymorphism detection. In particular, the invention provides PCR-based methods, with normalization of the amplified products using a duplex-specific nuclease, in order to reduce over-representation of PCR products. Oligonucleotides for use in the disclosed method are also provided.

Abstract: The present invention provides methods to produce a reduced representation of a genome for sequencing and DNA polymorphism detection. In particular, the invention provides PCR-based methods, with normalization of the amplified products using a duplex-specific nuclease, in order to reduce over-representation of PCR products. Oligonucleotides for use in the disclosed method are also provided.

Abstract: The present invention provides methods to produce a reduced representation of a genome for sequencing and DNA polymorphism detection. In particular, the invention provides PCR-based methods, with normalization of the amplified products using a duplex-specific nuclease, in order to reduce over-representation of PCR products. Oligonucleotides for use in the disclosed method are also provided.

Abstract: The present invention provides methods to produce a reduced representation of a genome for sequencing and DNA polymorphism detection. In particular, the invention provides PCR-based methods, with normalization of the amplified products using a duplex-specific nuclease, in order to reduce over-representation of PCR products. Oligonucleotides for use in the disclosed method are also provided.