Abstract: A cell and tissue culture device comprises a closed pressurization circuit (2) able to deliver a selected gas under a selected pressure, at least a culture chamber (6) accommodating means (13) for supporting at least a deformable membrane (14) forming in chamber (6) an interface between first (17) and second (16) portions with variable complementary volumes, wherein the first portion (17) is configured for receiving cells and/or tissues to be grown for being supplied with culture fluid by a first tank (8), and said second portion (16) is configured for being supplied with gas by the pressurization circuit (2) and for discharging at least a portion of this gas.

Abstract: A cell and tissue culture unit comprises a base (1) equipped with access means (7,8) for being connected to a culture medium feed device and delimiting at least side-walls (5) of a culture chamber (6) and at least side-walls of at least a well (3-i ) communicating through at least one of its upper and lower portions with chamber (6), as well as removable closing means (10,15-i,17i) able to provide access to chamber (6) and to well (3-i).

Abstract: A cell and tissue culture device comprises several culture wells (18-i) accommodating cells and tissues to be grown, of first (2) and second (25) tanks, and connecting means (20,23,24) coupled to wells and tanks so as to enable a culture fluid to flow from one tank to another via the wells. Each tank (20,25) accommodates at least a flexible pocket (6,7;27,29), at least one of which is able to receive the culture fluid. The device further comprises at least a controller module (50,150) providing first and/or second sequences of external pressures to be applied, on the pockets of first and second tanks, respectively, and pressurization means (41,46-49) configured for applying to the pockets, pressures defined by said first and second sequences provided by the controller module.