Patents by Inventor Lelita T. Braiterman

Lelita T. Braiterman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • In vitro transposition of article transposons
    Patent number: 5968785
    Abstract: We have developed efficient methods of creating artificial transposons and inserting these transposons into plasmid targets in vitro, primarily for the purpose of mapping and sequencing DNA. A plasmid has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into plasmid targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.
    Type: Grant
    Filed: February 6, 1997
    Date of Patent: October 19, 1999
    Assignee: The Johns Hopkins University
    Inventors: Scott E. Devine, Jef D. Boeke, Lelita T. Braiterman
  • Artificial transposons
    Patent number: 5843772
    Abstract: We have developed efficient methods of creating artificial transposons and inserting these transposons into DNA targets in vitro, primarily for the purpose of mapping and sequencing DNA. A target DNA has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into DNA targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.
    Type: Grant
    Filed: June 10, 1996
    Date of Patent: December 1, 1998
    Assignee: John Hopkins University
    Inventors: Scott E. Devine, Jef D. Boeke, Lelita T. Braiterman
  • In vitro transposition of artificial transposons for DNA sequencing
    Patent number: 5728551
    Abstract: We have developed efficient methods of creating artificial transposons and inserting these transposons into DNA targets in vitro, primarily for the purpose of mapping and sequencing DNA. A target DNA has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into DNA targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.
    Type: Grant
    Filed: March 2, 1995
    Date of Patent: March 17, 1998
    Assignee: The Johns Hopkins University
    Inventors: Scott E. Devine, Jef D. Boeke, Lelita T. Braiterman
  • In vitro transposition of artificial transposons
    Patent number: 5677170
    Abstract: We have developed efficient methods of creating artificial transposons and inserting these transposons into plasmid targets in vitro, primarily for the purpose of mapping and sequencing DNA. A plasmid has been engineered to convert virtually any DNA sequence, or combination of sequences, into an artificial transposon; hence, custom transposons containing any desired feature can be easily designed and constructed. Such transposons are then efficiently inserted into plasmid targets, in vitro, using the integrase activity present in yeast Ty1 virus-like particles. Primers complementary to the transposon termini can be used to sequence DNA flanking any transposon insertion.
    Type: Grant
    Filed: March 2, 1994
    Date of Patent: October 14, 1997
    Assignee: The Johns Hopkins University
    Inventors: Scott E. Devine, Jef D. Boeke, Lelita T. Braiterman