Patents by Inventor Leo Heijnen

Leo Heijnen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for generating oligonucleotides, in particular for the detection of amplified restriction fragments obtained using AFLP®
    Patent number: 7166429
    Abstract: The invention relates to a method for generating, and optionally detecting, an oligonucleotide, comprising at least the steps of: a) providing a first dsDNA; b) ligating the first dsDNA to a second dsDNA, in which said second dsDNA sequence comprises within its sequence at least one recognition site for an IIS restriction endonuclease; so as to provide a ligated dsDNA; d) restricting the ligated dsDNA with the at least one restriction endonuclease of the IIS type so as to obtain at least a first and a second IIS-restricted dsDNA; and optionally comprising the further step of: e) detecting the first and/or second IIS-restricted dsDNA obtained in the restriction step d). Preferably, said method comprises the further step of: c) amplifying the ligated dsDNA obtained in the ligating step b) prior to the restriction step d).
    Type: Grant
    Filed: December 28, 2000
    Date of Patent: January 23, 2007
    Assignee: Keygene N.V.
    Inventors: Michael Josephus Theresia Van Eijk, René Cornelis Josephus Hogers, Leo Heijnen
  • Method for generating oligonucleotides, in particular for the detection of amplified restriction fragments obtained using aflp
    Publication number: 20030175729
    Abstract: The invention relates to a method for generating, and optionally detecting, an oligonucleotide, comprising at least the steps of: a) providing a first dsDNA; b) ligating the first dsDNA to a second dsDNA, in which said second dsDNA sequence comprises within its sequence at least one recognition site for an IIS restriction endonuclease; so as to provide a ligated dsDNA; d) restricting the ligated dsDNA with the at least one restriction endonuclease of the IIS type so as to obtain at least a first and a second IIS-restricted dsDNA; and optionally comprising the further step of: e) detecting the first and/or second IIS-restricted dsDNA obtained in the restriction step d). Preferably, said method comprises the further step of: c) amplifying the ligated dsDNA obtained in the ligating step b) prior to the restriction step d).
    Type: Application
    Filed: January 16, 2003
    Publication date: September 18, 2003
    Inventors: Michael Josephus Theresia Van Eijk, Rene Cornelis Josephus Hogers, Leo Heijnen