Patents by Inventor Leon Xu
Leon Xu has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240117425Abstract: The invention provides compositions and methods for sequencing nucleic acids and other applications. In sequencing by synthesis, unlabeled reversible terminators are incorporated by a polymerase in each cycle, then labeled after incorporation by binding to the reversible terminator a directly or indirectly labeled antibody or other affinity reagent.Type: ApplicationFiled: September 7, 2023Publication date: April 11, 2024Inventors: Radoje Drmanac, Snezana Drmanac, Handong Li, Xun Xu, Matthew J. Callow, Leon Eckhardt, Naibo Yang
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Patent number: 9657337Abstract: Embodiments of the present invention relate to a buffer composition for an integrated nucleic acid amplification and hybridization reaction. The buffer comprise about 50-200 mM of a salt, about 10-30 mM Tris-HCl, about 2-10M Water soluble magnesium salt, about 0.05-1.5% surfactant, about 0.05-0.15 mg/ml stabilizing protein about 50-300 nM of one or more primers, about 20-150 uM of one or more dNTPs, about 5-15% glycerine, about 0.5-1.5% formamide and at least about 5 unit/ml polymerase.Type: GrantFiled: December 29, 2006Date of Patent: May 23, 2017Assignee: Honeywell International Inc.Inventors: Leon Xu, Zhenhong Sun
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Patent number: 8822677Abstract: An improved two stage reaction process for production of mono-L-aspartyl chlorin e6. In a first stage, the activation reaction between chlorin e6 and a carbodiimide produces a previously unknown anhydride in an activation reaction product (Formula I). This reaction product is purified to remove a significant proportion of the precursors of di-L-aspartyl chlorin e6. The purified activation reaction product contains a higher concentration of the previously unknown anhydride. This purified reaction product is used in a second stage: a coupling reaction of the purified activation reaction product with aspartate. The coupling reaction produces a coupling reaction product that has significantly reduced di-L-aspartyl chlorin e6 concentration. This reduced di-L-aspartyl chlorin e6 concentration facilitates purification of mono-L-aspartyl chlorin e6 from the coupling reaction mixture.Type: GrantFiled: May 16, 2012Date of Patent: September 2, 2014Assignee: Purdue Pharmaceutical Products L.P.Inventors: Leon Xu, Alexander J. Pallenberg
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Patent number: 8741565Abstract: A method for detecting a target nucleic acid of a pathogen in a test sample, the method comprising preparing a target nucleic acid detecting reagent and contacting the target nucleic acid detecting reagent with an oligonucleotide microarray. A kit for detecting a target nucleic acid of a pathogen in a test sample is also described. The kit comprises at least one primer pair and an oligonucleotide microarray comprising at least one probe.Type: GrantFiled: December 28, 2006Date of Patent: June 3, 2014Assignee: Honeywell International Inc.Inventors: Yuandong Gu, Leon Xu
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Publication number: 20130281689Abstract: An improved two stage reaction process for production of mono-L-aspartyl chlorin e6. In a first stage, the activation reaction between chlorin e6 and a carbodiimide produces a previously unknown anhydride in an activation reaction product. This reaction product is purified to remove a significant proportion of the precursors of di-L-aspartyl chlorin e6. The purified activation reaction product contains a higher concentration of the previously unknown anhydride. This purified reaction product is used in a second stage: a coupling reaction of the purified activation reaction product with aspartate. The coupling reaction produces a coupling reaction product that has significantly reduced di-L-aspartyl chlorin e6 concentration. This reduced di-L-aspartyl chlorin e6 concentration facilitates purification of mono-L-aspartyl chlorin e6 from the coupling reaction mixture.Type: ApplicationFiled: May 16, 2012Publication date: October 24, 2013Inventors: Leon Xu, Alexander J. Pallenberg
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Patent number: 8367397Abstract: Embodiments of the invention relate to an active biochip for nucleic acid analysis. The biochip comprises an inlet for introducing a nucleic acid sample, fluid channels, valves in contact with the fluid channels and pumps in contact with the fluid channels and adapted to generate a carrier gas or move a buffer through a portion of the fluid channels. The biochip also includes one or more hydroxyapatite columns for separating a portion of the nucleic acid sample, buffer reservoirs in contact with the fluid channels and positioned near the pumps, air exits, a waste reservoir and a nucleic acid analysis region.Type: GrantFiled: February 15, 2007Date of Patent: February 5, 2013Assignee: Honeywell International Inc.Inventors: Yuandong Gu, Leon Xu
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Patent number: 8198312Abstract: A composition of matter of an anhydride of formula 1 below and an improved two stage reaction process for production of mono-L-aspartyl chlorin e6, utilizing the anhydride:Type: GrantFiled: June 29, 2007Date of Patent: June 12, 2012Assignee: Light Sciences Oncology, Inc.Inventors: Leon Xu, Alexander J. Pallenberg
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Patent number: 8124944Abstract: A microarray reader (100) comprises a light source (102), beam shaping elements (104) positioned near the light source (102), a moving stage (124) supporting one or more of the light source (102) and beam shaping elements (104), an optical substrate (112) supporting an immobilized microarray, a reaction chamber (116) in contact with the optical substrate (112) and encapsulating buffer solution, a heating/cooling component (118) in contact with the reaction chamber (116), a synchronization circuit, an optical filter (108) and an imaging sensor (106) positioned near the optical filter (108).Type: GrantFiled: January 17, 2007Date of Patent: February 28, 2012Assignee: Honeywell International Inc.Inventors: Yuan Zheng, Leon Xu, Zhenhong Sun
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Patent number: 8116508Abstract: An apparatus uses a transducer to produce vibration in the ultrasonic frequency range and in the audible frequency range. A membrane or cantilever structure is coupled to the transducer to produce acoustic waves. When the vibration is in the audible frequency range, the membrane structure works like a conventional loudspeaker. When the vibration is in the ultrasonic frequency range, the ultrasonic signal is modulated by audio signal for creating better directivity. The acoustic waves in the ultrasonic frequency range can reproduce directional audible sound due to the nonlinear interaction of ultrasonic waves in air.Type: GrantFiled: September 26, 2008Date of Patent: February 14, 2012Assignee: Nokia CorporationInventors: Liangfeng Leon Xu, Chenguang Cai, Jani K. Nurminen, Xia S. Wang, Antti O. Salo, Koray Ozcan
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Patent number: 8063203Abstract: An improved two stage reaction process for production of mono-L-aspartyl chlorin e6. In a first stage, the activation reaction between chlorin e6 and a carbodiimide produces a previously unknown anhydride in an activation reaction product. This reaction product is purified to remove a significant proportion of the precursors of di-L-aspartyl chlorin e6. The purified activation reaction product contains a higher concentration of the previously unknown anhydride. This purified reaction product is used in a second stage: a coupling reaction of the purified activation reaction product with aspartate. The coupling reaction produces a coupling reaction product that has significantly reduced di-L-aspartyl chlorin e6 concentration. This reduced di-L-aspartyl chlorin e6 concentration facilitates purification of mono-L-aspartyl chlorin e6 from the coupling reaction mixture.Type: GrantFiled: November 1, 2010Date of Patent: November 22, 2011Assignee: Light Sciences Oncology, Inc.Inventors: Leon Xu, Alexander J. Pallenberg
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Publication number: 20110105745Abstract: An improved two stage reaction process for production of mono-L-aspartyl chlorin e6. In a first stage, the activation reaction between chlorin e6 and a carbodiimide produces a previously unknown anhydride in an activation reaction product. This reaction product is purified to remove a significant proportion of the precursors of di-L-aspartyl chlorin e6. The purified activation reaction product contains a higher concentration of the previously unknown anhydride. This purified reaction product is used in a second stage: a coupling reaction of the purified activation reaction product with aspartate. The coupling reaction produces a coupling reaction product that has significantly reduced di-L-aspartyl chlorin e6 concentration. This reduced di-L-aspartyl chlorin e6 concentration facilitates purification of mono-L-aspartyl chlorin e6 from the coupling reaction mixture.Type: ApplicationFiled: November 1, 2010Publication date: May 5, 2011Applicant: Light Sciences Oncology, Inc.Inventors: Leon Xu, Alexander J. Pallenberg
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Publication number: 20100279885Abstract: A method for detecting a target nucleic acid of a pathogen in a test sample, the method comprising preparing a target nucleic acid detecting reagent and contacting the target nucleic acid detecting reagent with an oligonucleotide microarray. A kit for detecting a target nucleic acid of a pathogen in a test sample is also described. The kit comprises at least one primer pair and an oligonucleotide microarray comprising at least one probe.Type: ApplicationFiled: December 28, 2006Publication date: November 4, 2010Applicant: HONEYWELL INTERNATIONAL INC.Inventors: Yuandong Gu, Leon Xu
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Publication number: 20100197517Abstract: Embodiments of the present invention relate to a buffer composition for an integrated nucleic acid amplification and hybridization reaction. The buffer comprise about 50-20 OmM of a salt, about 10-30 mM Tris-HCI, about 2-10M Water soluble magnesium salt, about 0.05-1.5% surfactant, about 0.05-0.15 mg/ml stabilizing protein about 50-300 nM of one or more primers, about 20-15 OuM of one or more dNTPs, about 5-15% glycerine, about 0.5-1.5% formamide and at least about 5 unit/ml polymerase.Type: ApplicationFiled: December 29, 2006Publication date: August 5, 2010Applicant: HONEYWELL INTERNATIONAL INC.Inventors: Leon Xu, Zhenhong Sun
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Publication number: 20100148092Abstract: A microarray reader (100) comprises a light source (102), beam shaping elements (104) positioned near the light source (102), a moving stage (124) supporting one or more of the light source (102) and beam shaping elements (104), an optical substrate (112) supporting an immobilized microarray, a reaction chamber (116) in contact with the optical substrate (112) and encapsulating buffer solution, a heating/cooling component (118) in contact with the reaction chamber (116), a synchronization circuit, an optical filter (108) and an imaging sensor (106) positioned near the optical filter (108).Type: ApplicationFiled: January 17, 2007Publication date: June 17, 2010Applicant: Honeywell International Inc.Inventors: Yuan Zheng, Leon Xu, Zhenhong Sun
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Publication number: 20100080409Abstract: An apparatus uses a transducer to produce vibration in the ultrasonic frequency range and in the audible frequency range. A membrane or cantilever structure is coupled to the transducer to produce acoustic waves. When the vibration is in the audible frequency range, the membrane structure works like a conventional loudspeaker. When the vibration is in the ultrasonic frequency range, the ultrasonic signal is modulated by audio signal for creating better directivity. The acoustic waves in the ultrasonic frequency range can reproduce directional audible sound due to the nonlinear interaction of ultrasonic waves in air.Type: ApplicationFiled: September 26, 2008Publication date: April 1, 2010Inventors: Liangfeng Leon Xu, Chenguang Cai, Jani K. Nurminen, Xia S. Wang, Antti O. Salo, Koray Ozcan
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Patent number: 7629115Abstract: The present invention relates to an apparatus for testing multiple sample compounds for their biological effect comprising a porous block having substantially planar top and bottom surfaces. The top surface comprises a plurality of cell adhesive regions and cell dis-adhesive regions and the bottom surface provides multiple sites to load the sample compounds. These sites are located opposite from the cell adhesive regions on the top surface of the porous block. In certain embodiments, the invention further comprises at least one dissolvable layer which provides multiple sites to load the sample compounds.Type: GrantFiled: May 13, 2005Date of Patent: December 8, 2009Assignee: Honeywell International Inc.Inventors: Yuandong Gu, Leon Xu, Jicang Zhou
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Publication number: 20080199862Abstract: Embodiments of the invention relate to an active biochip for nucleic acid analysis. The biochip comprises an inlet for introducing a nucleic acid sample, fluid channels, valves in contact with the fluid channels and pumps in contact with the fluid channels and adapted to generate a carrier gas or move a buffer through a portion of the fluid channels. The biochip also includes one or more hydroxyapatite columns for separating a portion of the nucleic acid sample, buffer reservoirs in contact with the fluid channels and positioned near the pumps, air exits, a waste reservoir and a nucleic acid analysis region.Type: ApplicationFiled: February 15, 2007Publication date: August 21, 2008Inventors: Yuandong Gu, Leon Xu
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Publication number: 20080199861Abstract: Embodiments of the invention relate to a real-time microarray apparatus comprising an upper substrate, a lower substrate, a buffer positioned between the upper and lower substrate, a microarray positioned on either the upper substrate or the lower substrate, a heater positioned near the microarray, a pump positioned near the buffer and microarray and an imaging sensor positioned near the microarray.Type: ApplicationFiled: February 15, 2007Publication date: August 21, 2008Inventors: Yuandong Gu, Leon Xu
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Publication number: 20080176768Abstract: A method of creating a metal nanoparticle hydrogel biological array comprises placing a plurality of metal nanoparticles in each of a plurality of wells on a substrate, and placing a biomolecular probe material into the plurality of wells. Hydrogel precursors are placed in each of the plurality of wells; and are polymerized to form hydrogel such that the metal nanoparticles are embedded in the hydrogel.Type: ApplicationFiled: January 23, 2007Publication date: July 24, 2008Inventors: Yuan Zheng, Jicang Zhou, Leon Xu
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Publication number: 20080021210Abstract: An improved two stage reaction process for production of mono-L-aspartyl chlorin e6. In a first stage, the activation reaction between chlorin e6 and a carbodiimide produces a previously unknown anhydride in an activation reaction product. This reaction product is purified to remove a significant proportion of the precursors of di-L-aspartyl chlorin e6. The purified activation reaction product contains a higher concentration of the previously unknown anhydride. This purified reaction product is used in a second stage: a coupling reaction of the purified activation reaction product with aspartate. The coupling reaction produces a coupling reaction product that has significantly reduced di-L-aspartyl chlorin e6 concentration. This reduced di-L-aspartyl chlorin e6 concentration facilitates purification of mono-L-aspartyl chlorin e6 from the coupling reaction mixture.Type: ApplicationFiled: June 29, 2007Publication date: January 24, 2008Applicant: LIGHT SCIENCES ONCOLOGY, INC.Inventors: Leon Xu, Alexander Pallenberg