Abstract: Compositions, methods and kits are provided. The compositions, methods and kits are for assembly of series of DNA segments in yeast using homologous recombination. The assembled DNA segments are maintained episomally. Yeast made using the methods are included, as are methods of using the yeast to express proteins, and for screening test agents that can affect yeast that are modified to include the assembled DNA segments.

Abstract: The present disclosure provides methods, compounds, and systems for synthesizing a nucleic acid molecule. The methods may comprise spatially separating a set of barcoded oligonucleotides corresponding to a target nucleic acid molecule and performing a nucleic acid assembly using the oligonucleotides. A computer system coupled to a process control software program may be configured to apparatuses and configured to carry out the methods of the present disclosure.

Abstract: Provided are compositions and methods for compound discovery. Modified yeast that have their endogenous yeast shikimate pathway disrupted or deleted, and replaced with homologous pathway genes from one or more distinct organisms, are provided and used in assays of test agents. The homologous pathway genes are designed to supplement the disrupted or deleted shikimate pathway genes. The assays are designed to identify whether or not the test agents can interfere with the function of enzymes in the shikimate pathway from organisms that are distinct from the yeast avatar hosts. In embodiments, the disruption/deletion of the yeast endogenous shikimate pathway results in the yeast being incapable of producing chorismic acid.

Abstract: Provided are compositions and methods for compound discovery. Modified yeast that have their endogenous yeast shikimate pathway disrupted or deleted, and replaced with homologous pathway genes from one or more distinct organisms, are provided and used in assays of test agents. The homologous pathway genes are designed to supplement the disrupted or deleted shikimate pathway genes. The assays are designed to identify whether or not the test agents can interfere with the function of enzymes in the shikimate pathway from organisms that are distinct from the yeast avatar hosts. In embodiments, the disruption/deletion of the yeast endogenous shikimate pathway results in the yeast being incapable of producing chorismic acid.

Abstract: Compositions, methods and kits are provided. The compositions, methods and kits are for assembly of series of DNA segments in yeast using homologous recombination. The assembled DNA segments are maintained episomally. Yeast made using the methods are included, as are methods of using the yeast to express proteins, and for screening test agents that can affect yeast that are modified to include the assembled DNA segments.

Abstract: The present invention relates to the field of chromosome engineering. More specifically, the present invention provides methods and compositions useful for inducibly linearizing circular DNA molecules in vivo in yeast. In one embodiment, a comprises a nucleic acid encoding a selectable marker, wherein the nucleic acid encoding a selectable marker comprises an intron comprising an endonuclease recognition site flanked by telomere seed sequences.

Abstract: Provided are compositions and methods for use in assembling and expressing a plurality of transcription units using, in one aspect, homologous recombination in yeast. Yeast containing the plurality of transcription units, and isolated transcription units, are also provided. Kits for use in making the yeast and the transcript units are further included.

Abstract: Provided are compositions and methods for use in assembling and expressing a plurality of transcription units using, in one aspect, homologous recombination in yeast. Yeast containing the plurality of transcription units, and isolated transcription units, are also provided. Kits for use in making the yeast and the transcript units are further included.

Abstract: Provided are compositions and methods for use in assembling and expressing a plurality of transcription units using, in one aspect, homologous recombination in yeast. Yeast containing the plurality of transcription units, and isolated transcription units, are also provided. Kits for use in making the yeast and the transcript units are further included.

Abstract: Provided are compositions and methods for use in assembling and expressing a plurality of transcription units using, in one aspect, homologous recombination in yeast. Yeast containing the plurality of transcription units, and isolated transcription units, are also provided. Kits for use in making the yeast and the transcript units are further included.

Abstract: The present invention relates to the field of chromosome engineering. More specifically, the present invention provides methods and compositions useful for inducibly linearizing circular DNA molecules in vivo in yeast. In one embodiment, a comprises a nucleic acid encoding a selectable marker, wherein the nucleic acid encoding a selectable marker comprises an intron comprising an endonuclease recognition site flanked by telomere seed sequences.

Abstract: Fluoropolymer resin is stabilized and/or whitened by addition of a small amount of alkali metal nitrate prior to extrusion. The nitrate can be introduced during polymerization, isolation, or preparation of extruder feed.

Abstract: A vaccine produced in edible plant and/or animal products, as well as a method of second-generation vaccine development through the production of at least one complete structure of a pathogen in a transgenic plant or animal is described. Preferably, the present invention enables the production of virus-like particles in edible food plants, through the co-expression of a plurality of proteins and/or of a plurality of portions of such proteins. The co-expression of viral structural proteins should enhance the immunogenicity of the transgenic antigen by providing all elements necessary to induce a protective immune response. In one variation of the method the immune response to the transgenic vaccine would be induced by presenting the transgenic proteins as part of an edible plant structure, utilizing this to stimulate the mucosal immune system. Alternatively, the vaccine produced in transgenic plant/animal products could be purified and used as an immunogen in other vaccination strategies.

Abstract: An apparatus and method for evacuating and sealing a bag made of a sealable material and containing a comminuted product. The bag is positioned with its open upper end around a sleeve through which a hollow probe can be extended. A pair of upper jaw members close to form an outer temporary seal above an intermediate region of the bag above the level of the surface of the charge in the bag where a permanent seal is to be formed. A pair of lower jaws members close to form an inner temporary seal about a lower region of the bag above the surface of the charge. With the probe extended through the sleeve into the bag, the bag is evacuated through the probe. After evacuation is completed and after withdrawal of the hollow probe, a pair of sealing members close against the intermediate region of the bag in order to form a permanent seal for the bag.

Abstract: An apparatus and method for evacuating and sealing a bag made of a sealable material and containing a comminuted product. The bag is positioned with its open upper end around a sleeve through which a hollow probe can be extended. A pair of upper jaw members close to form an outer temporary seal above an intermediate region of the bag above the level of the surface of the charge in the bag where a permanent seal is to be formed. A pair of lower jaws members close to form an inner temporary seal about a lower region of the bag above the surface of the charge. With the probe extended through the sleeve into the bag, the bag is evacuated through the probe. After evacuation is completed and after withdrawal of the hollow probe, a pair of sealing members close against the intermediate region of the bag in order to form a permanent seal for the bag.

Abstract: An apparatus and method are disclosed for evacuating and sealing a bag (133) made of a sealable material and containing a charge (134) of a comminuted or other flowable product, such as roasted and ground coffee. The bag (133) is positioned with its open upper end around a sleeve (12) through which a hollow probe (14) can be extended. A pair of upper jaw members (UJ) close to form an outer temporary seal about an upper region of the bag above an intermediate region of the bag which is also above the level of the surface of the charge (134) in the bag (133) where a permanent seal is to be formed. A pair of lower jaw members (LJ) close to form an inner temporary seal about a lower region of the bag below the upper and intermediate regions and above the surface of the charge (134) in the bag (133). With the probe (14) extended through the sleeve (12) into the bag (133), the bag (133) is evacuated through the probe (14).

Abstract: An apparatus and method for evacuating and sealing a bag (133) made of a sealable material and containing a charge (134) of a comminuted or other flowable product, such as roasted and ground coffee. The bag (133) is positioned with its open upper end around a sleeve (12) through which a hollow probe (14) can be extended. A pair of upper jaw members (UJ) close to form an outer temporary seal about an upper region of the bag above an intermediate region of the bag which is also above the level of the surface of the charge (134) in the bag (133) where a permanent seal is to be formed. A pair of lower jaws members (LJ) close to form an inner temporary seal about a lower region of the bag below the upper and intermediate regions and above the surface of the charge (134) in the bag (133). With the probe (14) extended through the sleeve (12) into the bag (133), the bag (133) is evacuated through the probe (14).

Abstract: A variable capacity reservoir for cigarettes or similar rod-like articles comprises a pair of fixed upwardly extending helical guides located above the normal plane of conveyance of a laterally-flexible mass flow conveyor, and a drive carriage carrying wheels engageable with the conveyor and rotatable about the axis of the helical guides so as to reversibly deflect a variable length of conveyor around said guides. As the conveyor is advanced into or retracted from the helical guides, it is correspondingly transferred from or to a store arranged below the guides.

Abstract: A connection assembly for the cathode of an aluminum smelter pot (10) including at least one insert (30) received within a hole in the collector bar (16) of a collector bar/strap (16/15) connection for the cathode and in electrical contact with the collector bar (16) and in electrical contact with the strap (15) via tab (15a).

Abstract: Copolymers of tetrafluoroethylene, hexafluoropropylene, and perfluoro(ethyl vinyl ether) can be extruded at higher rates than corresponding copolymers containing perfluoro(propyl vinyl ether).