Patents by Inventor Liangjing Chen
Liangjing Chen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11971349Abstract: The present invention discloses a combined oxygen, nitrogen and hydrogen joint determination device and method based on thermal conductivity-infrared technique. In the device, a melting and extraction system comprises a carrier gas introduction pipeline, a chamber valve, an impulse furnace and a dust filter; an analytical gas transmission system comprises a flush valve, a bypass valve, a mass flowmeter and a first switching valve; a signal detection system comprises an infrared cell, an analysis purification reagent tube and a thermal conductivity cell; and a signal analysis system comprises a controller and a processor. The present invention realizes different joint determination modes by switching different gas paths, which can meet different determination requirements of different users.Type: GrantFiled: October 31, 2023Date of Patent: April 30, 2024Assignees: NCS Testing Technology CO., LTD, NCS Jiangsu Testing Technology Co., Ltd.Inventors: Yunhai Jia, Xuehua Wang, Liangjing Yuan, Danyang Zhi, Runpeng Wang, Xiaoke Hao, Zhenning Wu, Wang Chen, Zhaodong Guo, Xinyang Dong
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Publication number: 20220177949Abstract: The invention relates to methods of RNA amplification, including methods for the reverse transcription of cDNA from RNA using a thermostable reverse transcriptase. In a particular aspect, the methods are capable of linear amplification of an RNA template through multiple cycles of cDNA synthesis.Type: ApplicationFiled: December 20, 2021Publication date: June 9, 2022Inventors: Gary J. Latham, Richard Andrew Blidner, Liangjing Chen
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Patent number: 11236384Abstract: The invention relates to methods of RNA amplification, including methods for the reverse transcription of cDNA from RNA using a thermostable reverse transcriptase. In a particular aspect, the methods are capable of linear amplification of an RNA template through multiple cycles of cDNA synthesis.Type: GrantFiled: January 16, 2018Date of Patent: February 1, 2022Assignee: ASURAGEN, INC.Inventors: Gary J. Latham, Richard Andrew Blidner, Liangjing Chen
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Publication number: 20210189384Abstract: The present disclosure relates to methods and compositions for nucleic acid library preparation. In certain aspects, the present disclosure relates to methods of making a library of concatenated amplicons from a target nucleic acid. The present disclosure further relates to methods of using the methods and compositions described herein, e.g., in downstream applications such as sequencing (e.g., single-molecule sequencing), gene assembly, and/or structural variation characterization.Type: ApplicationFiled: November 25, 2020Publication date: June 24, 2021Applicant: Asuragen, Inc.Inventors: Gary J. LATHAM, Liangjing Chen
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Publication number: 20200140923Abstract: The invention relates to methods of RNA amplification, including methods for the reverse transcription of cDNA from RNA using a thermostable reverse transcriptase. In a particular aspect, the methods are capable of linear amplification of an RNA template through multiple cycles of cDNA synthesis.Type: ApplicationFiled: January 16, 2018Publication date: May 7, 2020Inventors: Gary J. Latham, Richard Andrew Blidner, Liangjing Chen
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Patent number: 9783848Abstract: This disclosure relates to methods of determining the presence and position of AGG or interruptor elements within a trinucleotide (for example, CGG) repeat region, and to methods of determining the number of repeats present in this region, by amplifying a set of products with a set of primers of which at least one comprises a portion of the CGG repeat region, and resolving the products to produce a representation of product size and abundance.Type: GrantFiled: February 26, 2014Date of Patent: October 10, 2017Assignee: Asuragen, Inc.Inventors: Gary J. Latham, Liangjing Chen, Sachin Sah
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Patent number: 9777330Abstract: Methods are provided for determining the methylation status of GC-rich templates. The methods include use of GC reference standards that allow simultaneous characterization of methylation status and CGG repeat length. The methods are useful for detecting genotypes associated with GC-rich repeats, including Fragile X Syndrome.Type: GrantFiled: October 28, 2011Date of Patent: October 3, 2017Assignee: Asuragen, Inc.Inventors: Gary J. Latham, Liangjing Chen, Andrew Hadd, Sachin Sah, Ru Cao
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Publication number: 20160160194Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.Type: ApplicationFiled: December 22, 2014Publication date: June 9, 2016Inventors: LIANGJING CHEN, ROBERT SETTERQUIST, GARY LATHAM
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Patent number: 8956841Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.Type: GrantFiled: December 28, 2012Date of Patent: February 17, 2015Assignee: Applied Biosystems, LLCInventors: Liangjing Chen, Robert Setterquist, Gary Latham
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Publication number: 20140248625Abstract: This disclosure relates to methods of determining the presence and position of AGG or interruptor elements within a trinucleotide (for example, CGG) repeat region, and to methods of determining the number of repeats present in this region, by amplifying a set of products with a set of primers of which at least one comprises a portion of the CGG repeat region, and resolving the products to produce a representation of product size and abundance.Type: ApplicationFiled: February 26, 2014Publication date: September 4, 2014Applicant: Asuragen, Inc.Inventors: Gary J. LATHAM, Liangjing CHEN, Sachin SAH
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Patent number: 8679757Abstract: This disclosure relates to methods of determining the presence and position of AGG or interruptor elements within a trinucleotide (for example, CGG) repeat region, and to methods of determining the number of repeats present in this region, by amplifying a set of products with a set of primers of which at least one comprises a portion of the CGG repeat region, and resolving the products to produce a representation of product size and abundance.Type: GrantFiled: February 16, 2010Date of Patent: March 25, 2014Assignee: Asuragen, Inc.Inventors: Gary J. Latham, Liangjing Chen, Sachin Sah
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Patent number: 8361754Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.Type: GrantFiled: September 28, 2009Date of Patent: January 29, 2013Assignee: Applied Biosystems, LLCInventors: Liangjing Chen, Robert A. Setterquist, Gary Latham
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Publication number: 20120107824Abstract: Methods are provided for determining the methylation status of GC-rich templates. The methods include use of GC reference standards that allow simultaneous characterization of methylation status and CGG repeat length. The methods are useful for detecting genotypes associated with GC-rich repeats, including Fragile X Syndrome.Type: ApplicationFiled: October 28, 2011Publication date: May 3, 2012Inventors: Gary J. LATHAM, Liangjing Chen, Andrew Hadd, Sachin Sah, Ru Cao
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Publication number: 20100243451Abstract: This disclosure relates to methods of determining the presence and position of AGG or interruptor elements within a trinucleotide (for example, CGG) repeat region, and to methods of determining the number of repeats present in this region, by amplifying a set of products with a set of primers of which at least one comprises a portion of the CGG repeat region, and resolving the products to produce a representation of product size and abundance.Type: ApplicationFiled: February 16, 2010Publication date: September 30, 2010Inventors: GARY J. LATHAM, LIANGJING CHEN, SACHIN SAH
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Publication number: 20100203597Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.Type: ApplicationFiled: September 28, 2009Publication date: August 12, 2010Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Liangjing Chen, Robert A. Setterquist, Gary Latham
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Patent number: 7595179Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.Type: GrantFiled: April 19, 2004Date of Patent: September 29, 2009Assignee: Applied Biosystems, LLCInventors: Liangjing Chen, Robert A. Setterquist, Gary J. Latham
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Publication number: 20050232934Abstract: The present invention relates to a gene that encodes a hyperactive reverse transcriptase having DNA polymerase activity and substantially reduced RNase H activity, vectors containing the gene and host cells transformed with the invention. The present invention also includes a method of producing the hyperactive reverse transcriptase, producing cDNA from mRNA using the reverse transcriptase of the invention, kits and assay templates made using the hyperactive reverse transcriptase.Type: ApplicationFiled: April 19, 2004Publication date: October 20, 2005Applicant: Ambion, Inc.Inventors: Liangjing Chen, Robert Setterquist, Gary Latham