Abstract: A solid medium of Antrodia cinnamomea comprises 20 to 50 wt % soybean; 30 to 50 wt % coixseed; and 20 to 50 wt % sorghum. A culturing method of Antrodia cinnamomea, comprises providing a solid medium comprising soybean, coixseed and sorghum; preparing a solution of Antrodia cinnamomea, inoculating the solution of Antrodia cinnamomea on the solid medium in a concentration of 1×103 to 1×106 cfu on per 100 g of solid medium, and statically culturing the solution of Antrodia cinnamomea on the solid medium till obtaining hyphae adhered on the solid medium; and culturing the solution of Antrodia cinnamomea by intermittent shaking in a frequency of every 5 to 7 days shaking 5 to 10 minutes, with a period of the culturing by intermittent shaking being more than 40 days.