Abstract: The present disclosure provides a method for separating a neural crest derived cell from peripheral blood. In the present disclosure, a mononuclear cell is separated from the peripheral blood and then directly cultured, thereby maximizing use of a neural crest stem cell with a differentiation potential to avoid loss of the neural crest stem cell. In the method of the present disclosure, a sample to be separated is derived from the peripheral blood. The method shows less trauma and low cost. Most importantly, compared to extracting the neural crest derived cell from tissues, the neural crest derived cell extracted from the peripheral blood can be used clinically as a type of biomarker.