Abstract: The present disclosure provides a method for analyzing the signal of a neutral atom imaging unit, including: preparing a neutral atom imaging unit, which includes a semiconductor detector array and modulation grids disposed at intervals in front of the semiconductor detector array; preparing a neutral atom source plane, energetic neutral atoms emitted by the neutral atom source plane are received by the semiconductor detector array after passing through the modulation grids, and the modulation grids form a projection on the semiconductor detector array; obtaining a response function of the imaging unit according to the projection; calculating the data signal obtained by the neutral atom imaging unit; and performing inversion imaging on the neutral atom emission source according to the response function of the imaging unit and the data signal. The method well inverts the neutral atom emission source to obtain the intensity and size of the neutral atom emission source.

Abstract: The present disclosure provides a direct current (DC) transformer error detection apparatus for a pulsating harmonic signal, including a DC and pulsating harmonic current output module and an external detected input module, where the DC and pulsating harmonic current output module outputs a DC and a DC superimposed pulsating harmonic current to an internal sampling circuit and a self-calibrated standard resistor array; and the internal sampling circuit converts the input DC and the input DC superimposed pulsating harmonic current into a voltage signal, and sends the voltage signal to an analog-to-digital (AD) sampling and measurement component through a front-end conditioning circuit and a detected input channel. The DC transformer error detection apparatus can complete self-calibration for measurement of the DC and the pulsating harmonic signal on a test site.

Abstract: A method of constructing a specific CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats associated) to activate a RSPO2 (R-spondin 2) gene is disclosed in the present invention. The method comprises the following steps: designing a sgRNA (single guide RNA) of a specifically targeted human RSPO2 gene; constructing a CRISPR-Cas9 recombinant lentivirus vector of a specifically activated RSPO2 gene; and lentiviral packaging a CRISPR-Cas9 system of the specifically activated RSPO2 gene. The CRISPR-Cas9 system designed by the present invention activates the RSPO2 target gene expression and promotes the activation of the hepatic stellate cell.

Abstract: Plural crRNA for detecting RSPO2 gene in body fluid with CRISPR-Cas13a specificity and applications thereof are disclosed. The crRNA is able to construct the CRISPR-Cas13a system and specifically detect micro-RSPO2 gene in the body fluid. The present invention is non-invasive and able to test rapidly, frequently and repeatedly. Compared to the conventional liquid biopsy, the present invention detects the micro-RSPO2 in the body fluid through the fluorescence units. The present invention has the advantages of no need for high-throughput sequencing, low cost and rapid testing speed, which is able to be adopted by large scale clinical applications.

Abstract: A method of constructing a specific CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats associated) to activate a RSPO2 (R-spondin 2) gene is disclosed in the present invention. The method comprises the following steps: designing a sgRNA (single guide RNA) of a specifically targeted human RSPO2 gene; constructing a CRISPR-Cas9 recombinant lentivirus vector of a specifically activated RSPO2 gene; and lentiviral packaging a CRISPR-Cas9 system of the specifically activated RSPO2 gene. The CRISPR-Cas9 system designed by the present invention activates the RSPO2 target gene expression and promotes the activation of the hepatic stellate cell.

Abstract: A method for knocking out a human RSPO2 gene targeted with CRISPR-Cas9 specificity includes steps of: 1) designing the sgRNA of the human RSPO2 gene targeted; and 2) constructing a CRISPR-Cas9 recombinant lentivirus vector for knocking out the RSPO2 gene. A method for preparing a lentiviral-packaged system for knocking out a human RSPO2 gene targeted with CRISPR-Cas9 specificity includes steps of: 1) designing the sgRNA of the human RSPO2 gene targeted; 2) constructing a CRISPR-Cas9 recombinant lentivirus vector for knocking out the RSPO2 gene; and 3) processing the CRISPR-Cas9 recombinant lentivirus vector for knocking out the sgRNA of the human RSPO2 gene with lentiviral packaging, so as to obtain the lentiviral-packaged system.