Abstract: Disclosed in the present invention are a primer-probe combination and a kit for vaginal microecosystem detection, wherein the primer-probe combination comprises primers and probes for target gene detection against Lactobacillus crispatus (LC), Lactobacillus gasseri (LG), Lactobacillus jensenii (LJ), Lactobacillus iners (LI), Gardnerella vaginalis (GV), Candida albicans (CA) and Trichomonas vaginalis (TV), and the probes are used for melting curve analysis. The kit comprises the primer-probe combination. The combination of specific primers and probes enables effective amplification of specific target genes of the above different microorganisms and melting curve analysis performed on the amplified products thereof, making detection of the above seven microorganisms in a single tube possible, thus achieving the advantages of high specificity, short time consuming, high sensitivity, comprehensive coverage of detection sites, etc.

Abstract: A single-stage multi-path direct current access converter and a control method thereof are provided. The single-stage multi-path direct current access converter includes an AC-side conversion circuit; at least two DC-side conversion circuits; and transformers corresponding to the at least two DC-side conversion circuits. AC sides of the at least two DC-side conversion circuits are respectively connected to primary windings of the corresponding transformers; secondary windings of the transformers are connected in parallel to a first side of the AC-side conversion circuit; each of the at least two DC-side conversion circuits is configured to invert DC power received by the DC-side conversion circuit, or to rectify AC power received by the DC-side conversion circuit; and the AC-side conversion circuit is configured to perform AC frequency conversion.

Abstract: The present invention discloses a combination of DNA methylation markers and the use thereof, primers, probes and a kit for early detection of ovarian cancer, wherein the combination of DNA methylation markers comprises at least one methylated fragment of each of four genes, including PCDHB18P, CDO1, HOXA9 and LYPD5. The detection primers and kit obtained for the methylated regions of the target genes can significantly improve the sensitivity of ovarian cancer detection, and meanwhile maintain a high specificity for normal ovarian cells and benign ovarian tumors. The detection primers and probes detect patients with possible gynecological malignant tumors early through molecular epigenetic methods using methylation detection technology, and the result is very accurate, and can provide clinicians with auxiliary diagnostic reference for early prophylactic treatment.

Abstract: Disclosed in the present invention are a primer-probe combination and a kit for vaginal microecosystem detection, wherein the primer-probe combination comprises primers and probes for target gene detection against Lactobacillus crispatus (LC), Lactobacillus gasseri (LG), Lactobacillus jensenii (LJ), Lactobacillus iners (LI), Gardnerella vaginalis (GV), Candida albicans (CA) and Trichomonas vaginalis (TV), and the probes are used for melting curve analysis. The kit comprises the primer-probe combination. The combination of specific primers and probes enables effective amplification of specific target genes of the above different microorganisms and melting curve analysis performed on the amplified products thereof, making detection of the above seven microorganisms in a single tube possible, thus achieving the advantages of high specificity, short time consuming, high sensitivity, comprehensive coverage of detection sites, etc.

Abstract: The present invention discloses a combination of DNA methylation markers and the use thereof, primers, probes and a kit for early detection of ovarian cancer, wherein the combination of DNA methylation markers comprises at least one methylated fragment of each of four genes, including PCDHB18P, CDO1, HOXA9 and LYPD5. The detection primers and kit obtained for the methylated regions of the target genes can significantly improve the sensitivity of ovarian cancer detection, and meanwhile maintain a high specificity for normal ovarian cells and benign ovarian tumors. The detection primers and probes detect patients with possible gynecological malignant tumors early through molecular epigenetic methods using methylation detection technology, and the result is very accurate, and can provide clinicians with auxiliary diagnostic reference for early prophylactic treatment.