Abstract: Provided herein is a sandwich immunoassay for detecting cross-linked PIIINP that has at least two strands of PIIINP joined together by inter-strand cross-linking each having a C-terminal neo-epitope of PIIINP that is generated by N-protease cleavage of intact type III procollagen. A biological sample having the cross-linked PIIINP is contacted with a first surface-bound monoclonal antibody and then by a second monoclonal antibody, both specifically reactive with a neoepitope in the C-terminal sequence of PIIINP, and then binding of the second monoclonal antibody is determined. Also provided is a method for evaluating the efficacy of an antagonist drug targeting lysyl oxidases via the immunoassay and a kit containing a solid support binding the first monoclonal antibody and containing the second monoclonal antibody.

Abstract: Provided herein is a sandwich immunoassay for detecting cross-linked PIIINP that has at least two strands of PIIINP joined together by inter-strand cross-linking each having a C-terminal neo-epitope of PIIINP that is generated by N-protease cleavage of intact type III procollagen. A biological sample having the cross-linked PIIINP is contacted with a first surface-bound monoclonal antibody and then by a second monoclonal antibody, both specifically reactive with a neoepitope in the C-terminal sequence of PIIINP, and then binding of the second monoclonal antibody is determined. Also provided is a method for evaluating the efficacy of an antagonist drug targeting lysyl oxidases via the immunoassay and a kit containing a solid support binding the first monoclonal antibody and containing the second monoclonal antibody.

Abstract: The present invention relates to a filter for liquid filtration, the construction of the filter ensure increased filtration capacity by using up the filtration medium rather that clogging up. The filter comprises layers of filtration medium (2) and optionally also layers of spacer medium (1). Liquid to be filtered can through bypass spaces circumvent a number of layers of filtration and/or spacer medium and is by at least one sealing (3) directed through the filtering area of filtration medium. End caps (5) with perforations (6) allow the liquid to be distributed within the bypass space between the edges of the layers of filtration medium and spacer medium. Rolled filters with a central core (4) to drain off the filtered liquid can be constructed in large dimensions. Also disclosed is a method making the filter. The filters are suitable for many purposes, especially for filtering liquid with oil.