Patents by Inventor Lloyd Ruddock

Lloyd Ruddock has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for producing natively folded proteins in a prokaryotic host
    Patent number: 9976164
    Abstract: The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds.
    Type: Grant
    Filed: December 9, 2015
    Date of Patent: May 22, 2018
    Assignee: University of Oulu
    Inventor: Lloyd Ruddock
  • Method for producing disulfide bond containing proteins in a prokaryotic cytoplasm
    Patent number: 9416388
    Abstract: The present invention relates to a method for producing natively folded disulfide bond containing proteins in a prokaryotic host. The method comprises that in the cytoplasm of a prokaryotic cell is expressed protein(s) of interest that naturally contain disulfide bonds and naturally occurring or inverted transmembrane enzyme, wherein the cysteines of the active site(s) are naturally or after genetic engineering located towards the prokaryotic cytoplasm. The enzyme is selected from the group of VKOR, inverted VKOR (iVKOR) and inverted Dsb B (iDsb B). In the prokaryotic cell is also expressed cytoplasmic DsbA or a corresponding protein being capable of providing electrons to the active site(s) of VKOR, iVKOR or iDsbB. The invention relates also to a prokaryotic host cell and a vector system for producing natively folded disulfide bond containing proteins.
    Type: Grant
    Filed: February 3, 2012
    Date of Patent: August 16, 2016
    Assignee: University of Oulu
    Inventors: Lloyd Ruddock, Feras Hatahet
  • Method for producing natively folded proteins in a prokaryotic host
    Publication number: 20160090615
    Abstract: The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds.
    Type: Application
    Filed: December 9, 2015
    Publication date: March 31, 2016
    Inventor: Lloyd Ruddock
  • Method for producing natively folded proteins in a prokaryotic host
    Patent number: 9238817
    Abstract: The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds.
    Type: Grant
    Filed: December 2, 2011
    Date of Patent: January 19, 2016
    Assignee: Univeristy of Oulu
    Inventor: Lloyd Ruddock
  • Method for producing disulfide bond containing proteins in a prokaryotic cytoplasm
    Publication number: 20140342398
    Abstract: The present invention relates to a method for producing natively folded disulfide bond containing proteins in a prokaryotic host. The method comprises that in the cytoplasm of a prokaryotic cell is expressed protein(s) of interest that naturally contain disulfide bonds and naturally occurring or inverted transmembrane enzyme, wherein the cysteines of the active site(s) are naturally or after genetic engineering located towards the prokaryotic cytoplasm. The enzyme is selected from the group of VKOR, inverted VKOR (iVKOR) and inverted Dsb B (iDsb B). In the prokaryotic cell is also expressed cytoplasmic DsbA or a corresponding protein being capable of providing electrons to the active site(s) of VKOR, iVKOR or iDsbB. The invention relates also to a prokaryotic host cell and a vector system for producing natively folded disulfide bond containing proteins.
    Type: Application
    Filed: February 3, 2012
    Publication date: November 20, 2014
    Applicant: UNIVERSITY OF OULU
    Inventors: Lloyd Ruddock, Feras Hatahet
  • Method for Producing Natively Folded Proteins in a Prokaryotic Host
    Publication number: 20120202245
    Abstract: The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds.
    Type: Application
    Filed: December 2, 2011
    Publication date: August 9, 2012
    Applicant: UNIVERSITY OF OULU
    Inventor: Lloyd Ruddock